Interferon-γ Release Assay and Reverse Blot Hybridization Assay: Diagnostic Role in Cutaneous Tuberculosis

Chung, Hee Chul; Kim, Bo Kyung; Hong, Hannah; Wang, Hye Young; Kim, Yeun; Lee, Hyeyoung; Eom, Minseob; Choi, Eung Ho

doi:10.2340/00015555-2182

Cited by 4 publications

(5 citation statements)

References 10 publications

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“…The molecular techniques used in previous reports to identify mycobacterial DNA from sarcoidosis lesions were primarily nested or real‐time PCR . However, REBA hybridizes the PCR products of the DNA extracted from specimens on to a fixed mycobacterial DNA template and thus exhibits increased sensitivity as it amplifies the PCR results . More sarcoidosis cases could be identified as Mycobacterium ‐associated by using REBA rather than conventional molecular techniques.…”

Section: Discussionmentioning

confidence: 99%

“…3 However, REBA hybridizes the PCR products of the DNA extracted from specimens on to a fixed mycobacterial DNA template and thus exhibits increased sensitivity as it amplifies the PCR results. 8 More sarcoidosis cases could be identified as Mycobacterium-associated by using REBA rather than conventional molecular techniques. Increasing incidents of mycobacterial DNA identification in cases, which have been clinically diagnosed as sarcoidosis could weaken the distinctions between sarcoidosis and TB.…”

Section: Discussionmentioning

confidence: 99%

“…The method has shown high sensitivity and specificity for identifying mycobacterial DNA and can differentiate between 18 different mycobacterial species, including MTB and non‐tuberculous mycobacteria (NTM) . REBA has been proven useful for identifying mycobacteria from the lesions of Mycobacterium ‐infected diseases …”

Section: Introductionmentioning

confidence: 99%

“…7 REBA has been proven useful for identifying mycobacteria from the lesions of Mycobacterium-infected diseases. [8][9][10] The aim of this study was to identify mycobacteria from cutaneous sarcoidosis lesions using REBA.…”

Section: Introductionmentioning

confidence: 99%

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Identification of Mycobacterium tuberculosis and non‐tuberculous mycobacteria from cutaneous sarcoidosis lesions by reverse blot hybridization assay

Lee

Eom

Kim

et al. 2019

The Journal of Dermatology

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While the etiology of sarcoidosis remains uncertain, mycobacteria have been suggested as a causative infectious agent. To investigate the causal relationship between mycobacteria and sarcoidosis, we performed a reverse blot hybridization assay (REBA) to identify mycobacteria from the skin samples of nine patients with sarcoidosis. Six of the nine samples were shown to be positive for mycobacteria by REBA, including Mycobacterium tuberculosis and non‐tuberculous mycobacteria. This is the first study to identify mycobacteria from the skin samples of sarcoidosis patients using REBA, and our results could strengthen the etiologic association between mycobacteria and sarcoidosis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification of Mycobacterium tuberculosis and non‐tuberculous mycobacteria from cutaneous sarcoidosis lesions by reverse blot hybridization assay

Lee

Eom

Kim

et al. 2019

The Journal of Dermatology

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“…A QuantiFERON-Tuberculosis Gold In-Tube test (Cellestis Inc., Valencia, USA) was also positive. Recently, these interferon-γ release assays provide a fast and reliable diagnoses of cutaneous as well as systemic tuberculosis with high specificity and sensitivity (1). She was diagnosed with tuberculous lymphadenitis and treated with anti-tuberculous medication (rifampicin, isoniazid, pyrazinamide, and ethambutol).…”

Section: Case Reportmentioning

confidence: 99%