Interferon-beta

Revel, Michel

doi:10.1016/s1874-5687(97)80034-1

Cited by 2 publications

(1 citation statement)

References 320 publications

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“…In this regard, recent studies have revealed that the mild dissolution of IB proteins by anionic detergents such as SDS has resulted in the retention of the secondary structure of the recombinant protein. 9,12 On the other hand, given the hydrophobic properties of INFβ-1b, 14,15 this variant has poor solubility in the aqueous solution in the absence of anionic detergents. 15,16 Thus, it seems that using SDS can be a suitable alternative for urea.…”

Section: Introductionmentioning

confidence: 99%

Solubilization of Human Interferon β-1b Inclusion Body Proteins by Organic Solvents

Nekoufar

Fazeli

2020

Adv Pharm Bull

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Purposes: Solubilization of inclusion bodies expressed in E. coli is a critical step during manufacturing of recombinant proteins expressed as inclusion bodies. So far, various methods have been used for solubilization and purification of inclusion body proteins to obtain active proteins with high purity and yield. The aim of this study was to examine the benefit of organic solvents such as alcohols in solubilization of recombinant interferon β-1b inclusion bodies. Methods: Effect of important parameters inclusion pH, concentration and type of denaturant and concentration of alcoholic solvents were optimized to formulate a suitable solubilization buffer and investigate their effect on solubilization of interferon β-1b inclusion bodies. Results: Our findings showed the acidic pH in the range of 2-3 is more suitable than alkaline pH >12 for solubilization and achieving higher content of interferon β-1beta and pure recombinant protein. We have also demonstrated that 1% SDS acts better than 2M urea to solubilize Inclusion body proteins of interferon β-1b at pH of 2-3. The interferon concentration was 2.35 mg per 100 mg IB when we used 40% (v/v) 1-propanol and 20% (v/v) 2-butanol into the buffer solution as well. Conclusion: The optimized method provides gentile condition for solubilization of inclusion body at high protein concentration and purity with a degree of retention of native secondary structure which makes this method valuable to be used in production and research area.

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Section: Introductionmentioning

confidence: 99%

Solubilization of Human Interferon β-1b Inclusion Body Proteins by Organic Solvents

Nekoufar

Fazeli

2020

Adv Pharm Bull

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Features of the solubilization of interferon beta-1B from inclusion bodies

2015

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A new solubilization method of recombinant interferon beta-1b (IFNβ-1b) from the inclusion bodies was developed. This method allows to extract the target protein selectively in the solutions of different alcohols, such as ethanol, propanol and isopropanol. It was shown that the more effective IFNβ-1b solubilization was achieved in the 55% propanol solution. This method allowed to extract the target protein from inclusion bodies around 85-90%, and significantly reduced Escherichia coli content in the solubilizate, in comparison with standard methods.

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Interferon-beta

Cited by 2 publications

References 320 publications

Solubilization of Human Interferon β-1b Inclusion Body Proteins by Organic Solvents

Solubilization of Human Interferon β-1b Inclusion Body Proteins by Organic Solvents

Features of the solubilization of interferon beta-1B from inclusion bodies

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