Interferences of o-raffinose cross-linked hemoglobin in three methods for serum creatinine

Abstract

The interferences of o-raffinose cross-linked hemoglobin (HemolinkTM) were examined and compared in two serum creatinine methods on the Hitachi 717 [Boehringer Mannheim (BMC) and Synermed] and in an enzymatic creatinine method on the Vitros 750 (Johnson & Johnson). Interference was considered significant when the change in creatinine concentration from the control exceeded the 95% confidence limits of each method. Significant interference was observed for the BMC/Hitachi 717 method with Hemolink ≥5 g/L. No interference was observed for the Synermed/Hitachi 717 assay with Hemolink up to 30 g/L in normal samples and up to 50 g/L in samples with increased creatinine. No interference was observed for the Vitros 750 assay with Hemolink up to 50 g/L at both normal and increased concentrations of creatinine. Although the BMC/Hitachi 717 method was considered unacceptable, the Synermed/Hitachi 717 and the Vitros 750 methods allow accurate quantification of serum creatinine in the presence of Hemolink.

Acute kidney injury in acute-on-chronic liver failure: where does hepatorenal syndrome fit?

“…In practice, both chemical and enzymatic methods are used to measure creatinine in body fluids, with most clinical laboratories in the US and UK currently using variations of the Jaffe reaction; a method of which there are multiple variations. Many of the modifications have been undertaken to improve the specificity of the reaction, as the Jaffe reaction is not specific for creatinine: other compounds that may produce a Jaffe-like chromogen include proteins, 73,74 glucose, ascorbic acid 75 ketone bodies, 76 pyruvate, guanidine, haemoglobin F, blood-substitute products 77 and cephalosporins. 78 The degree of interference varies between individuals but collectively may account for 20% of measured 'creatinine' at physiological concentrations.…”