Interference with jasmonic acid-regulated gene expression is a general property of viral suppressors of RNA silencing but only partly explains virus-induced changes in plant–aphid interactions

Westwood, Jack H.; Lewsey, Mathew G.; Murphy, Alex M.; Tungadi, Trisna; Bates, Anne; Gilligan, Christopher A.; Carr, John P.

doi:10.1099/vir.0.060624-0

Cited by 51 publications

(51 citation statements)

References 36 publications

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“…In these TuMV-infected plants, it was found that deposition of callose (which has defensive properties against aphids) was decreased and the levels of certain amino acids were elevated in the phloem sap and that these changes were attributable to the TuMV NIa-Pro (Casteel et al, 2014). In contrast, Westwood et al (2014) found that infection of N. benthamiana plants with PVY decreased the reproduction of Myzus persicae although the growth of the individual aphids placed on the infected plants was not inhibited. Although transgenic expression of the PVY HC-Pro in N. benthamiana inhibited jasmonic acid-induced plant gene expression (a potential anti-insect defense) and enhanced aphid reproduction, it was concluded that this viral factor is not the predominant determinant of virus-induced effects on aphid-plant interactions during infection (Westwood et al, 2014).…”

Section: Molecular Determinants Of Aphid-mediated Transmission and Homentioning

confidence: 79%

“…In contrast, Westwood et al (2014) found that infection of N. benthamiana plants with PVY decreased the reproduction of Myzus persicae although the growth of the individual aphids placed on the infected plants was not inhibited. Although transgenic expression of the PVY HC-Pro in N. benthamiana inhibited jasmonic acid-induced plant gene expression (a potential anti-insect defense) and enhanced aphid reproduction, it was concluded that this viral factor is not the predominant determinant of virus-induced effects on aphid-plant interactions during infection (Westwood et al, 2014). Currently, it is not known if BCMV or BCMNV alter the behavior or performance (survival, growth, or reproduction) of aphids on infected bean plants.…”

Section: Molecular Determinants Of Aphid-mediated Transmission and Homentioning

confidence: 79%

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Bean Common Mosaic Virus and Bean Common Mosaic Necrosis Virus

Worrall

Wamonje

Mukeshimana

et al. 2015

Advances in Virus Research

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Section: Molecular Determinants Of Aphid-mediated Transmission and Homentioning

confidence: 79%

Section: Molecular Determinants Of Aphid-mediated Transmission and Homentioning

confidence: 79%

Bean Common Mosaic Virus and Bean Common Mosaic Necrosis Virus

Worrall

Wamonje

Mukeshimana

et al. 2015

Advances in Virus Research

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“…Attenuated expression of JA‐responsive genes was previously reported for transgenic Nicotiana benthamiana plants expressing the HC‐Pro silencing suppressor that is employed by many potyviruses (Westwood et al . ), including SMV (Lim et al . ).…”

Section: Discussionmentioning

confidence: 99%

“…The enhanced performance of E. varivestis larvae on SMV-infected plants, and the corresponding feeding preferences of adult beetles, may reflect the suppression of endogenous JA levels by SMV infection. Attenuated expression of JA-responsive genes was previously reported for transgenic Nicotiana benthamiana plants expressing the HC-Pro silencing suppressor that is employed by many potyviruses (Westwood et al 2014), including SMV (Lim et al 2011). As noted above, SMV-infected leaf tissues also had the highest levels of sugars of any treatment, along with increased levels of free amino acids in leaves, which are known feeding stimulants (Lapidus et al 1963;Augustine et al 1964).…”

Section: S I N G L E I N F E C T I O N B Y S M Vmentioning

confidence: 95%

Effects of single and mixed infections of Bean pod mottle virus and Soybean mosaic virus on host‐plant chemistry and host–vector interactions

et al. 2016

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Summary Co‐infection by vector‐borne plant viruses is common, yet few studies have explored the effects of mixed infections on host‐plant phenotypes or plant–vector interactions. We documented the effects of single and mixed infection by Bean pod mottle virus (BPMV) and Soybean mosaic virus (SMV) on key biochemical plant traits and the behaviour and performance of virus vectors (BPMV: Epilachna varivestis, SMV: Aphis glycines) in order to understand how virus‐induced changes in plant phenotypes might influence (i) the acquisition and transmission of each virus by its respective vector in single infections, (ii) the likelihood of secondary infection for plants singly infected with either virus and (iii) the implications of co‐infection for virus transmission by vectors. Single infection by either BPMV or SMV increased host‐plant palatability for E. varivestis, potentially enhancing vector acquisition of BPMV from BPMV‐infected plants as well as the risk of secondary BPMV infection in plants infected with SMV. However, co‐infected plants were no more palatable to beetle vectors than mock‐inoculated plants. BPMV infection had minimal impacts on A. glycines. SMV infection reduced A. glycines population growth, but increased aphid feeding preferences for infected plants, a pattern likely not conducive to the (non‐persistent) transmission of SMV. Co‐infection eliminated the negative effects of single SMV infection on aphid population growth, and aphids exhibited a feeding preference for co‐infected (relative to mock‐inoculated) plants. Our results demonstrate that virus effects on host phenotype and vector behaviour can be modified by the presence of a co‐infecting virus, with potentially important implications for disease transmission.

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“…Signaling pathways controlling these plant responses to microbial infection operate through the action of phytohormones like jasmonic acid (JA). During viral infection, however, JA-regulated gene expression is rather suppressed by RNA silencing (Hanks et al, 2005;Westwood et al, 2014). As a consequence, virus-infected plants may be more susceptible to secondary bacterial or fungal infections.…”

Section: Resultsmentioning

confidence: 99%

Differentially expressed genes in healthy and plum pox virus-infected Nicotiana benthamiana plants

Vozárová¹,

Žilová²,

Šubr³

2015

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Summary. -Viruses use both material and energy sources of their hosts and redirect the production of disposable compounds in order to make viral replication more effi cient. Metabolism of infected organisms is modifi ed by these enhanced requirements as well by their own defense response. Resulting complex story consists of many regulation events on various gene expression levels. Elucidating these processes may contribute to the knowledge on virus-host interactions and to evolving new antiviral strategies. In our work we applied a subtractive cloning technique to compare the transcriptomes of healthy and plum pox virus (PPV)-infected Nicotiana benthamiana plants. Several genes were found to be induced or repressed by the PPV infection. Th e induced genes were mainly related to general stress response or photosynthesis, several repressed genes could be connected with growth defects evoked by the infection. Interestingly, some genes usually up-regulated by fungal or bacterial infection were found repressed in PPV-infected plants. Potential involvement of particular diff erently expressed genes in the process of PPV infection is discussed.Keywords: virus-host interaction; gene expression; subtractive hybridization * Corresponding author. E-mail: virusubr@savba.sk; phone: +421-2-59302447. Abbreviations: APX = ascorbate peroxidase; CAB = chlorophyll a/b-binding protein; DSPP = dentin sialophosphoprotein; FRL = Frigida-like protein; GCS = glycine cleavage system; HMGB = high mobility group B protein; JA = jasmonic acid; MLP = major latex protein; MT = metallothionein; PI-PLC = phosphoinositide-specifi c phospholipase C; PPV = plum pox virus; PRG = photosynthesis-related gene; PS II = photosystem II; ROS = reactive oxygen species; SNS = S-norcoclaurine synthase; SUMO = small ubiquitin-related modifi er; TLP = tetraspanin-like protein; Ub = ubiquitin

show abstract

Interference with jasmonic acid-regulated gene expression is a general property of viral suppressors of RNA silencing but only partly explains virus-induced changes in plant–aphid interactions

Cited by 51 publications

References 36 publications

Bean Common Mosaic Virus and Bean Common Mosaic Necrosis Virus

Bean Common Mosaic Virus and Bean Common Mosaic Necrosis Virus

Effects of single and mixed infections of Bean pod mottle virus and Soybean mosaic virus on host‐plant chemistry and host–vector interactions

Differentially expressed genes in healthy and plum pox virus-infected Nicotiana benthamiana plants

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