Interference of intradermal tuberculin tests on the serodiagnosis of paratuberculosis in cattle

Varges, Renato; Marassi, Carla Dray; Oelemann, Walter; Lilenbaum, Walter

doi:10.1016/j.rvsc.2008.08.006

Cited by 27 publications

(34 citation statements)

References 5 publications

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“…The Johnin skin test for paratuberculosis, which is interpreted as positive when skin thickness increased ≥4 mm, is a specific and low-cost test for the early diagnosis of paratuberculosis in the majority of dairy herds, although the specificity of both tests is influenced by batch of PPD antigen and varies among herds (Kalis et al 2003). Although very few studies (n=3) in the present review determined prevalences using this type of tests, previous information from Brazilian studies suggests that the intradermal tuberculin tests can interfere in the reliability of ELISA (Lilenbaun et al 2007(Lilenbaun et al , 2009Varges et al 2009) and that serological testing for PTB should be avoided for 90 days after PPD inoculation ). Studies on paratuberculosis in Latin America and the Caribbean rarely reported tuberculosis status or previous tuberculin tests before paratuberculosis testing, which could suggest that results could be higher than reported.…”

Section: Discussionmentioning

confidence: 73%

Systematic review of the prevalence of paratuberculosis in cattle, sheep, and goats in Latin America and the Caribbean

Silva

Correa-Valencia

Ramı́rez

2014

Trop Anim Health Prod

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Mycobacterium avium subsp. paratuberculosis causes paratuberculosis or Johne's disease (JD) in domestic ruminants and wild species. The aim of the present study was to systematically review the prevalence of paratuberculosis among farmed animals (cattle, sheep, and goats) in Latin America and the Caribbean. The initial search for existing publications reporting systematic reviews and primary studies was carried out by searching the available databases. For the final selection of studies, an initial screen for basic eligibility and a detailed appraisal of quality were performed. After study selection, the relevant data were extracted. The detailed appraisal generated 24 publications that reported 52 studies, of which 73.1, 11.5, and 15.4 % were from cattle, sheep, and goats, respectively. Thirty-three (63.5 %) of the studies were animal level studies, while 19 (36.5 %) were herd-/flock-level studies. No flock-level studies on prevalence in sheep were found. Studies in Latin American and Caribbean countries revealed an overall prevalence of 16.9 (95 % CI (confidence interval) 13.2-20.5) and 75.8 % (95 % CI 50.1-101.5) in cattle at the animal and herd levels, respectively; the prevalence was 16 % (95 % CI 7.9-24.1) in sheep at the animal level and 4.3 % (95 % CI 1.9-6.8) and 3.7 % (95 % CI 0.1-7.4) in goats at the animal and flock levels, respectively. In general, prevalence results reported by the studies were insufficient to accurately determine the prevalence of paratuberculosis in farmed animals in Latin America and the Caribbean. Several flaws in the design of studies limit the quality of evidence regarding the prevalence of paratuberculosis in the region.

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Section: Discussionmentioning

confidence: 73%

Systematic review of the prevalence of paratuberculosis in cattle, sheep, and goats in Latin America and the Caribbean

Silva

Correa-Valencia

Ramı́rez

2014

Trop Anim Health Prod

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“…Two international studies, one conducted in Brazil (21), and the second in the UK (24), have previously shown that tests for bTB interfere with MAP ELISA diagnostics. Varges et al (21) reported ELISA interference occurring between 30 and 90 days post-PPD administration in bTB and MAP negative cattle.…”

Section: Discussionmentioning

confidence: 99%

“…MAP infection can interfere with specificity of bTB diagnostics (19), and likewise M. bovis infection can affect MAP serological tests (20). Varges et al (21), has also shown interference by both single and comparative intradermal bTB tests on MAP sero diagnostics in bTB negative animals. The primary purpose of this current study was to investigate the impact of SICCT on the prevalence of ELISA positive results (serum and milk) in an Irish herd containing both MAP ELISA seropositive and seronegative animals over a period of 6 months.…”

Section: Introductionmentioning

confidence: 98%

The Single Intradermal Cervical Comparative Test Interferes with Johneâ€™s Disease ELISA Diagnostics

et al. 2014

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Enzyme-linked immunosorbent assays (ELISA) of milk and serum samples are a routinely used method of screening herds for Mycobacterium avium subspecies paratuberculosis (MAP). Infection with MAP causes granulomatous enteritis of ruminants known as Johne’s disease (JD). The sensitivity (Se) and specificity (Sp) of MAP ELISAs leads to difficulties in the identification of both infected and infectious animals. Interference with MAP ELISA Se and Sp has been reported in MAP seronegative cows following administration of purified protein derivative (PPD) as part of intradermal testing for bovine tuberculosis (bTB). The aim of this study is to examine the impact of the single intradermal cervical comparative test (SICCT) for bTB, on both serum and milk MAP ELISA tests, in a herd containing both seropositive and seronegative cows pre-SICCT. A secondary objective is to provide appropriate timing of JD ELISA tests in relation to the SICCT. A herd of 139 cows were serum and milk sampled pre- and post-SICCT administration. Prior to SICCT, 6% of the herd tested seropositive for MAP using milk ELISA, with 8% positive on serum. ID Screen Paratuberculosis Indirect Screening Test (ID Vet) was used to screen the herd. Within 14 days of PPD administration, a significant increase in the prevalence of seropositive cows was recorded. Identical prevalence’s were recorded with both test matrices (39%). ELISA values remained significantly higher until day 43 post-SICCT in milk (P = 0.850), and day 71 in serum (P = 0.602). If the “new” positives detected post-bTB testing are deemed false positives due to generation of cross-reacting antibodies by administration of PPD, milk would appear a more suitable sample for JD ELISA testing within 2 months of SICCT. In summary, sampling for JD utilizing milk ELISA should be avoided in the 43-day period following PPD administration, with serum ELISA sampling avoided for an additional 28 days.

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“…Therefore, some of our ELISA-A-positive results could be false positive, especially in those animals in which ELISA-B (absorbed test) and PCR produced negative results. Likewise, it is possible that some of the positive ELISA results have been produced due to the interference with tuberculin from intradermal test applied occasionally to some animals, in order to declare herds as free from bovine tuberculosis (Varges et al 2009). …”

Section: Discussionmentioning

confidence: 99%

Serological and molecular detection of Mycobacterium avium subsp. paratuberculosis in cattle of dairy herds in Colombia

Silva

Abdulmawjood

Akineden

et al. 2011

Trop Anim Health Prod

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The objective of this study is the detection of Mycobacterium avium subsp. paratuberculosis (MAP) by serum enzyme-linked immunosorbent assay (ELISA), fecal polymerase chain reaction (PCR), and fecal culture in Colombian dairy herds. Serum and fecal samples from asymptomatic cows (n = 307) of 14 dairy herds were tested for MAP by an unabsorbed ELISA test (ELISA-A). Serum and fecal samples from positive ELISA-A animals (n = 31) were further tested by an absorbed ELISA test (ELISA-B) and PCR. Fecal samples from animals of herds positive by ELISA-A and PCR (n = 105) were inoculated onto three different culture media. ELISA-A produced positive results in 10% of the serum samples and 71% of the herds. ELISA-B and PCR results were positive in two and six serum and fecal samples from positive ELISA-A animals, respectively. Fecal samples were negative for MAP on all culture media. The results of this study confirmed the presence of MAP in local dairy herds and the difficulties of MAP detection in asymptomatic animals by ELISA, PCR, and fecal culture.

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Interference of intradermal tuberculin tests on the serodiagnosis of paratuberculosis in cattle

Cited by 27 publications

References 5 publications

Systematic review of the prevalence of paratuberculosis in cattle, sheep, and goats in Latin America and the Caribbean

Systematic review of the prevalence of paratuberculosis in cattle, sheep, and goats in Latin America and the Caribbean

The Single Intradermal Cervical Comparative Test Interferes with Johneâ€™s Disease ELISA Diagnostics

Serological and molecular detection of Mycobacterium avium subsp. paratuberculosis in cattle of dairy herds in Colombia

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