Interference in adhesion of bacteria and yeasts isolated from explanted voice prostheses to silicone rubber by rhamnolipid biosurfactants

Abstract

Aims: The effects and extent of adhesion of four different bacterial and two yeast strains isolated from explanted voice prostheses to silicone rubber with and without an adsorbed rhamnolipid biosurfactant layer obtained from Pseudomonas aeruginosa DS10-129 was studied. Methods and Results: The ability of rhamnolipid biosurfactant to inhibit adhesion of micro-organisms to silicone rubber was investigated in a parallel-plate flow chamber. The anti-adhesive activity of the biosurfactant at different concentrations was significant against all the strains and depended on the micro-organism tested. The results showed an effective reduction in the initial deposition rates, and the number of bacterial cells adhering after 4 h, for all micro-organisms tested at the 4 g l )1 undiluted rhamnolipid solution. Maximum initial reduction of adhesion rate (an average of 66%) occurred for Streptococcus salivarius GB 24/9 and Candida tropicalis GB 9/9. The number of cells adhering after 4 h on silicone rubber conditioned with biosurfactant was reduced to 48% for Staphylococcus epidermidis GB 9/6, Strep. salivarius GB 24/9, Staphylococcus aureus GB 2/1 and C. tropicalis GB 9/9 in comparison to controls. Perfusing the flow chamber with biosurfactant containing solution followed by the passage of a liquid-air interface, to investigate detachment of micro-organisms adhering to silicone rubber, produced high detachment (96%) of adhered cells for all microorganisms studied, except for Staph. aureus GB 2/1 (67%). Significance and Impact of the Study: It is concluded that biosurfactant represent suitable compounds that should be considered in developing future strategies to prevent the microbial colonization of silicone rubber voice prostheses. is uncomfortable, costly, time consuming and may lead to damage of the shunt with scar tissue formation, insufficiency or stenosis. Less frequent replacements of voice prosthesis therefore would be advantageous and has resulted in general interest in finding techniques to inhibit biofilm formation and prolonging the lifetime of voice prostheses Elving et al. 2000; Rodrigues et al. 2004a,b). Everaert et al. (1999) studied, in vivo, the influence of perfluoroalkylsiloxane (PA) surface modification of silicone rubber voice prostheses on biofouling, with 18 consecutive patients with laryngectomies, and found that chemisorption of long-chain PAs reduces biofilm formation. Additionally, Rodrigues et al. (2004a) developed a promising strategy to lengthen lifetime of the voice prostheses as it was demonstrating that the use of biosurfactants obtained from probiotic bacteria inhibit biofilm formation and the occurrence of increased airflow resistances.Surfactants of microbial origin, referred to as biosurfactants, are surface-active compounds that have some influence on interfaces. With regard to an anti-adhesive effect of biosurfactants, hypotheses have been forwarded in which adsorption of biosurfactants to a substratum surface alters the hydrophobicity of the surface and causes interference in m...

Biosurfactants and Bioemulsifiers Biomedical and Related Applications – Present Status and Future Potentials

“…Several reports have suggested that, in addition to their direct action against pathogens, biosurfactants are able to interfere with biofilm formation, modulating microbial interaction with interfaces (Federle & Bassler 2003;Merk et al, 2005;Neu, 1996;Rasmussen & Givskov, 2006;Rodrigues et al, 2006bRodrigues et al, , 2006cRodrigues et al, 2007). Surfactin for example has shown to be an important part of a list of biofilm controlling agents.…”

Biossurfactantes: propriedades anticorrosivas, antibiofilmes e antimicrobianas

“…Superfícies de borracha de silicone condicionadas com ramnolipídios de P. aeruginosa, obtiveram inibições na adesão de 60,9 %, 53,1 %, 58,2 % e 33,8 % contra as bactérias R. dentocariosa, S. epidermidis, S. salivarius e S. aureus, respectivamente, com solução de ramnolipídio não diluída (4 g/L). 97 99 Estes dados reforçaram a ideia de que alguns biossurfactantes são efetivos na inibição da adesão de acordo com as características das superfícies. Foi comprovado que a B. kururiensis possui o sistema HSL de quorum sensing, chamado de BraI/R, que produz e responde ao 3-oxo-C 12 -HSL e é extremamente similar ao LasI/R de P. aeruginosa (nesta é interconectado com outros sistemas regulatórios e é o regulador global envolvido na regulação de muitos genes que possuem importância na virulência e colonização).…”

Avaliação da capacidade de produção de biofilmes e detecção da enzima KPC em Salmonella spp. isoladas de aviário e linha de abate de aves

“…Dessa forma, a partir dos resultados encontrados, pode-se afirmar que o método do AVC para as estirpes de Salmonella spp., utilizadas neste experimento não pode ser considerado como teste presuntivo válido para a avaliação da produção de biofilme, conforme vem sendo utilizado para estirpes de S. aureus cuja eficiência já é comprovada (STEPANOVIC et al, 2004;RODRIGUES et al, 2006) …”

“…strains isolated from aviary and slaughter line of chicken in a fridge of Rio Grande do Sul state to produce biofilms and be resistant to carbapenems antibiotics. In the evaluation of biofilm production, it was used the polyethylene microplates and capsule production technique according to Stepanovic et al (2004) and Rodrigues et al (2006), respectively. The virulence factors of salmonella were researched, represented by the genes IpfA, agfA, sefA, invA, hilA, avrA, sopE, sivH,e spvC, using the Polymerase Chain Reaction (PCR) method described by Borges et al (2013).…”

“…Assim sendo, a presente pesquisa objetivou a verificação da capacidade de estirpes de Salmonella spp isoladas de aviário e linha de abate de frangos de um frigorífico no estado do Rio Grande do Sul produzirem biofilmes e apresentarem resistência a antibióticos carbapenêmicos. Na avaliação da produção de biofilme, foi empregada a técnica de microplacas de polietileno e produção de cápsula segundo Stepanovic et al (2004) e Rodrigues et al (2006), respectivamente.Foram pesquisados os fatores de virulência de salmonelas, representados pelos genes IpfA, agfA, sefA, invA, hilA, avrA, sopE, sivH,e spvC, utilizando o método de Reação de cadeia de Polimerase (PCR), descrita por Borges et al (2013). As estirpes foram submetidas ao teste de resistência a antibióticos carbapenêmicos pelo método de disco difusão com carbapenêmicos segundo Clinical and Laboratory Standards Institute-CLSI (2010) e pesquisa do gene de resistência a carbapenêmicos bla KCP , pela técnica de PCR, segundo NAAS et al (2007).…”