Interfacing microchip isoelectric focusing with on-chip electrospray ionization mass spectrometry

Nordman, Nina; Laurén, Susanna; Kotiaho, Tapio; Franssila, Sami; Kostiainen, Risto; Sikanen, Tiina

doi:10.1016/j.chroma.2015.04.031

Cited by 14 publications

(11 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nordman et al. demonstrated interfacing of microchip IEF with online MS detection via a fully integrated, on‐chip sheath‐flow ESI emitter . The inherent EOF taking place in SU‐8 photoresist microchannels with surface charges at a high pH was exploited for electrokinetic mobilization of the focused pH gradient toward MS.…”

Section: Development Of Methodology and Instrumentationmentioning

confidence: 99%

Recent advances in CE‐MS coupling: Instrumentation, methodology, and applications

2016

View full text Add to dashboard Cite

This review focuses on the latest development of microseparation electromigration methods in capillaries and microfluidic devices coupled with MS for detection and identification of important analytes. It is a continuation of the review article on the same topic by Kleparnik (Electrophoresis 2015, 36, 159-178). A wide selection of 161 relevant articles covers the literature published from June 2014 till May 2016. New improvements in the instrumentation and methodology of MS interfaced with capillary or microfluidic versions of zone electrophoresis, isotachophoresis, and isoelectric focusing are described in detail. The most frequently implemented MS ionization methods include electrospray ionization, matrix-assisted desorption/ionization and inductively coupled plasma ionization. Although the main attention is paid to the development of instrumentation and methodology, representative examples illustrate also applications in the proteomics, glycomics, metabolomics, biomarker research, forensics, pharmacology, food analysis, and single-cell analysis. The combinations of MS with capillary versions of electrochromatography, and micellar electrokinetic chromatography are not included.

show abstract

Section: Development Of Methodology and Instrumentationmentioning

confidence: 99%

Recent advances in CE‐MS coupling: Instrumentation, methodology, and applications

2016

View full text Add to dashboard Cite

show abstract

“…7.54 [36]) and their electrophoretic mobilities in aqueous buffers. [37] In non-aqueous background electrolytes, the change in the solvents' ε/η ratio (see Supplementary material, Table S2) may have a favorable effect on the resolving power and separation selectivity. Moreover, the electroosmotic flow of non-aqueous background electrolytes is typically slower than that of aqueous electrolytes leaving more time for the compounds to resolve electrophoretically even in relatively short separation channels.…”

Section: Capillary Zone Electrophoresis In Aqueous and Non-aqueous Comentioning

confidence: 99%

Aqueous and non-aqueous microchip electrophoresis with on-chip electrospray ionization mass spectrometry on replica-molded thiol-ene microfluidic devices

Tähkä

Bonabi

Jokinen

et al. 2017

Journal of Chromatography A

View full text Add to dashboard Cite

This work describes aqueous and non-aqueous capillary electrophoresis on thiol-ene-based microfluidic separation devices that feature fully integrated and sharp electrospray ionization (ESI) emitters. The chip fabrication is based on simple and low-cost replica-molding of thiol-ene polymers under standard laboratory conditions. The mechanical rigidity and the stability of the materials against organic solvents, acids and bases could be tuned by adjusting the respective stoichiometric ratio of the thiol and allyl ("ene") monomers, which allowed us to carry out electrophoresis separation in both aqueous and non-aqueous (methanol- and ethanol-based) background electrolytes. The stability of the ESI signal was generally ≤10% RSD for all emitters. The respective migration time repeatabilities in aqueous and non-aqueous background electrolytes were below 3 and 14% RSD (n=4-6, with internal standard). The analytical performance of the developed thiol-ene microdevices was shown in mass spectrometry (MS) based analysis of peptides, proteins, and small molecules. The theoretical plate numbers were the highest (1.2-2.4×10m) in ethanol-based background electrolytes. The ionization efficiency also increased under non-aqueous conditions compared to aqueous background electrolytes. The results show that replica-molding of thiol-enes is a feasible approach for producing ESI microdevices that perform in a stable manner in both aqueous and non-aqueous electrophoresis.

show abstract

“…Nordman et al. demonstrated the interfacing of microchip CIEF with online MS detection via a fully integrated, on‐chip sheath flow electrospray ionization (ESI) emitter. Relatively short focusing times of 60–270 s were sufficient for establishment of the pH gradient and CIEF separation of the sample components, yielding total analysis times well below 10 min.…”

Section: Mode Of Separationmentioning

confidence: 99%

Recent advances in microscale separation

et al. 2017

View full text Add to dashboard Cite

Recent advances in microscale separationThis review focuses on the recent advances on microscale separation technology, particularly on the new development on column and detection technologies in electrophoretic and chromatographic separation modes (i.e., CE, CEC, cLC, etc.). Representative examples illustrate also applications on the small molecules, peptides, proteins, polysaccharides, chiral and so on. 246 relevant articles are cited covering the literature published

show abstract

Interfacing microchip isoelectric focusing with on-chip electrospray ionization mass spectrometry

Cited by 14 publications

References 23 publications

Recent advances in CE‐MS coupling: Instrumentation, methodology, and applications

Recent advances in CE‐MS coupling: Instrumentation, methodology, and applications

Aqueous and non-aqueous microchip electrophoresis with on-chip electrospray ionization mass spectrometry on replica-molded thiol-ene microfluidic devices

Recent advances in microscale separation

Contact Info

Product

Resources

About