Interfacing Functionalized Carbon Nanohorns with Primary Phagocytic Cells

Lacotte, Stéphanie; García, A.; Décossas, Marion; Al-Jamal, Wafa' T; Li, Shouping; Kostarelos, Kostas; Muller, Sylviane; Prato, Maurizio; Dumortier, Hélène; Bianco, Alberto

doi:10.1002/adma.200702753

Cited by 47 publications

(27 citation statements)

References 31 publications

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“…S3) (44,45). The low CNH toxicity was also confirmed in both in vitro (41,44,45) and in vivo studies (41)(42)(43). We found that BSA-CNH has lower cytotoxicity than BSA-CNTs (Fig.…”

Section: Resultssupporting

confidence: 75%

“…4E). Immunohistochemical staining revealed the absence of undesired inflammatory responses, although CNH uptake by macrophages (41,42) stained with rabbit anti-ionized calcium binding adaptor molecule 1 was observed (53) (Fig. 4F).…”

Section: Resultsmentioning

confidence: 98%

“…In addition, Lacotte et al (41) reported activation of immunocompetent cells by chemically functionalized CNHs. Thus, further detailed studies on the biocompatibility of CNHs (e.g., high doses and long-term use) are needed to obtain a conclusive answer.…”

Section: Resultsmentioning

confidence: 99%

“…Potential problems exist with CNT biocompatibility, however, including impurities in the metal catalysts used in CNT synthesis (39,40). Conversely, CNHs are produced without metal catalysts and have relatively low toxicity, as shown in both in vitro and in vivo experiments (41)(42)(43)(44)(45). Thus, CNHs appear to be more amenable than CNTs for use in various biomedical and biotechnological applications.…”

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confidence: 99%

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Photothermic regulation of gene expression triggered by laser-induced carbon nanohorns

Miyako

Deguchi

Nakajima

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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The development of optical methods to control cellular functions is important for various biological applications. In particular, heat shock promoter-mediated gene expression systems by laser light are attractive targets for controlling cellular functions. However, previous approaches have considerable technical limitations related to their use of UV, short-wavelength visible (vis), and infrared (IR) laser light, which have poor penetration into biological tissue. Biological tissue is relatively transparent to light inside the diagnostic window at wavelengths of 650–1,100 nm. Here we present a unique optical biotechnological method using carbon nanohorn (CNH) that transforms energy from diagnostic window laser light to heat to control the expression of various genes. We report that with this method, laser irradiation within the diagnostic window resulted in effective heat generation and thus caused heat shock promoter-mediated gene expression. This study provides an important step forward in the development of light-manipulated gene expression technologies.

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“…S3) (44,45). The low CNH toxicity was also confirmed in both in vitro (41,44,45) and in vivo studies (41)(42)(43). We found that BSA-CNH has lower cytotoxicity than BSA-CNTs (Fig.…”

Section: Resultssupporting

confidence: 75%

Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

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Photothermic regulation of gene expression triggered by laser-induced carbon nanohorns

Miyako

Deguchi

Nakajima

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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“…Until now, studies at the cellular level on SWCNHs were mainly focused on the interaction between SWCNHs and non-polarized cells particularly macrophages, and have mostly emphasized on the cellular uptake and intracellular trafficking, but seldom on transportation. [14][15][16][17][18][19][20][21] It is worth noting that, depending on the routes of administration, nanocarriers may have to cross different physiological barriers in their journey towards their target. 22,23 These barriers are mainly polarized epithelia, such as the gastrointestinal tract, the lungs, and the blood-brain barrier.…”

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confidence: 99%

The interactions of single-wall carbon nanohorns with polar epithelium

Shi¹,

Shi

Li³

et al. 2017

IJN

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Single-wall carbon nanohorns (SWCNHs), which have multitudes of horn interstices, an extensive surface area, and a spherical aggregate structure, offer many advantages over other carbon nanomaterials being used as a drug nanovector. The previous studies on the interaction between SWCNHs and cells have mostly emphasized on cellular uptake and intracellular trafficking, but seldom on epithelial cells. Polar epithelium as a typical biological barrier constitutes the prime obstacle for the transport of therapeutic agents to target site. This work tried to explore the permeability of SWCNHs through polar epithelium and their abilities to modulate transcellular transport, and evaluate the potential of SWCNHs in drug delivery. Madin-Darby canine kidney (MDCK) cell monolayer was used as a polar epithelial cell model, and as-grown SWCNHs, together with oxidized and fluorescein isothiocyanate-conjugated bovine serum albumin-labeled forms, were constructed and comprehensively investigated in vitro and in vivo. Various methods such as transmission electron microscopy and confocal imaging were used to visualize their intracellular uptake and localization, as well as to investigate the potential transcytotic process. The related mechanism was explored by specific inhibitors. Additionally, fast multispectral optoacoustic tomography imaging was used for monitoring the distribution and transport process of SWCNHs in vivo after oral administration in nude mice, as an evidence for their interaction with the intestinal epithelium. The results showed that SWCNHs had a strong bioadhesion property, and parts of them could be uptaken and transcytosed across the MDCK monolayer. Multiple mechanisms were involved in the uptake and transcytosis of SWCNHs with varying degrees. After oral administration, oxidized SWCNHs were distributed in the gastrointestinal tract and retained in the intestine for up to 36 h probably due to their surface adhesion and endocytosis into the intestinal epithelium. Overall, this comprehensive investigation demonstrated that SWCNHs can serve as a promising nanovector that can cross the barrier of polar epithelial cells and deliver drugs effectively.

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Carbon Nanohorns and Their Biomedical Applications

Zhu

2011

Nanotechnologies for the Life Sciences

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The sections in this article are Introduction Structure and Properties Functionalization Covalent Functionalization Noncovalent Functionalization Biomedical Applications Toxicity Assessment of SWCNHs SWCNHs Used in Drug‐Delivery Systems SWCNHs Used in Magnetic Resonance Analysis Biosensing Applications of SWCNHs Conclusions Acknowledgments

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Interfacing Functionalized Carbon Nanohorns with Primary Phagocytic Cells

Cited by 47 publications

References 31 publications

Photothermic regulation of gene expression triggered by laser-induced carbon nanohorns

Photothermic regulation of gene expression triggered by laser-induced carbon nanohorns

The interactions of single-wall carbon nanohorns with polar epithelium

Carbon Nanohorns and Their Biomedical Applications

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