Interfacial microscopic examination and chemical analysis of resin-dentin interface of self-adhering flowable resin composite

Abstract

The newly introduced self-adhering flowable resin-composites Background: decrease the required time for application by incorporation of an acidic adhesive monomer, thus reducing the number of the steps, but its bonding is still uncertain. The aim of this study was to evaluate the interfacial microscopic examination and chemical analysis at the resin-dentin interface of a self-adhering flowable resin composite (Vertise-Flow) versus a total-etch (Te-Econom Plus) resin composite, using an etching agent (Eco-Etch gel) and bonding agent (Single Bond Universal).Sixteen freshly extracted sound human posterior teeth were used. Methods:The teeth were randomly divided into two groups: 8 specimens per type of composite. Standard-shaped class V cavities were prepared on the buccal surface. One group was restored by Te-Econom Plus resin composite by total-etch technique using Eco-Etch gel, which was applied to dentine for 15 seconds, followed by rinsing, drying and bonding agent application (Single Bond Universal). The other group restored directly with self-adhering resin composite (Vertise-Flow) without application of etch or bond. Curing was done for 20 seconds using a light emitting diode light curing unit. Evaluation of the resin-dentin interface was done microscopically by examination of marginal gap distance in μm using scanning electron microscope (SEM), and chemical analysis of silver particles was observed using SEM with energy-dispersive X-ray spectrometry after 24 hours of specimen storage in ammoniacal silver nitrate.Regarding marginal gap distance (µm) and silver atomic % mean Results: values, teeth restored with self-adhering resin composite (Vertise-Flow) showed significantly higher mean values than the multi-step etch and rinse resin composite group (5.2 0; 12.2 8.2, respectively).vs vs Resin-dentin bonding using total-etch resin composite technique Conclusions: was more effective than self-adhering flowable resin composite (Vertise-Flow) regarding marginal gap formation and penetration of silver particles. Further studies for bond strength could be performed.

Comparative Evaluation of Resin Dentin Interface using Universal and Total- Etch Adhesive Systems on Sound and Eroded Dentin: In Vitro Study

“…artifacts as compared light microscopy. 13 CLSM technique requires very low concentration of fluorochromes, which are soluble in the adhesive liquids without altering their properties. 13,23,32 CLSM was chosen in this study as it can generate noninvasive serial optical sectioning of intact specimens eliminating the artifacts arising with manual sectioning as seen in scanning electron microscopy (SEM) and transmission electron microscopy techniques (TEM).…”

“…13 CLSM technique requires very low concentration of fluorochromes, which are soluble in the adhesive liquids without altering their properties. 13,23,32 CLSM was chosen in this study as it can generate noninvasive serial optical sectioning of intact specimens eliminating the artifacts arising with manual sectioning as seen in scanning electron microscopy (SEM) and transmission electron microscopy techniques (TEM). 23 CLSM was also reported to render a detailed information than the SEM because of its nondestructive nature and elimination of other artifacts such as shrinking, swelling, or detachments that can happen with SEM.…”

“…11,12 Of late, self-adhering flowable resin composites were introduced eliminating the prerequisite of application of etch and bond. 13 However, various authors have reported more adhesive failures compared with cohesive failures in eroded teeth. 14,15 The adhesive techniques are critical, and the success of direct restorations is impeded by inadequate adhesion and hybrid layer degradation.…”

Interfacial microscopic examination and chemical analysis of resin-dentin interface of self-adhering flowable resin composite

“…Dataset 1: Raw values for silver atomic % in teeth treated with total-etch technique (Group A) and self-adhering resin composite (Group B) (n=8/group/method). doi, 10.5256/f1000research.12306.d177061 12 …”

“…Dataset 2: Scanning electron microscopy (SEM) showing gap formation (raw values included on the images) and SEM/energy-dispersive X-ray analysis (EDX) at the resin-superficial dentin interface in teeth treated with total-etch technique (Group A) and self-adhering resin composite (Group B) (n=8/group/method). doi, 10.5256/f1000research.12306.d177062 13 …”