Intérêt taxonomiquedes acides gras des mycobactéries: Proposition d'une méthode d'analyse

Structural elucidation and genomic scrutiny of the C60–C100 mycolic acids of Segniliparus rotundus

“…Whole cells were treated with a mixture of 40 % KOH and methoxyethanol (1 : 7,v/v) at 110 uC for 3 h in a screw-capped tube (Daffé et al, 1983). After acidification, FAs were extracted with diethyl ether and methylated with an ethereal solution of diazomethane, to yield mycolic acid methyl esters (MAMEs).…”

“…The absence of oxygenated mycolic acids in all isolates of the genus Segniliparus is a feature shared by mycobacterial species such as Mycobacterium fallax, Mycobacterium triviale and Mycobacterium chelonae/abscessus (Daffé et al, 1983;Barry et al, 1998). The methyl esters of a + -, a-and a9-mycolic acids (Fig.…”

“…In this respect, the Mycobacterium chelonae/abscessus complex was considered because their mycolic acids exclusively consist of a-and a9-mycolic acids . On the other hand, Mycobacterium marinum was examined as a reference for a mycolic acid content comparable to that of M. tuberculosis in terms of a-, keto-and methoxy-mycolic acids (Daffé et al, 1983(Daffé et al, , 1991. A survey of the kas and had gene organization from the available M. abscessus and M. marinum genomes points to the absence of the second kas gene in the fabD cluster of M. abscessus as in Segniliparus, while the same gene organization is found in M. marinum and M. tuberculosis (Table 3).…”

Identification of Mycobacteria by Thin Layer Chromatographic Analysis of Mycolic Acids and Conventional Biochemical Method: Four Years of Experience

“…Mycolic acid extraction and methylation procedures -The mycolic acid analysis was carried out according to the method proposed by Daffé et al (1983), with some modifications. About 25-50 mg of mycobacteria, derived from LowensteinJensen medium, were dispersed into a 5% (w/v) potassium hydroxide solution in 2-methoxyethanol.…”

“…Mycolic acids of the reference strains spotted together served as landmarks for the identification of mycolic acids extracted from the studied strains. One dimensional analysis was carried out by using two different elution systems: diethyl ether/petroleum ether (12:88 v/v), with three developments of the chromatogram, and dichlorometane with a single development (Daffé et al 1983). The presence of the separated components was revealed by spraying the chromatograms with 0, 01% (w/v) rhodamine in phosphate buffer (Daffé et al 1983).…”