Interdependency of freezing and thawing rates on cryopreserved human t cells

Baboo, J.; Kilbride, Peter; Delahaye, M.; Milne, Simon; Fonseca, Fernanda; Blanco, Magdalena; Meneghel, Julie; Nancekievill, Alex; Gaddum, Nicholas; Morris, John

doi:10.1016/j.cryobiol.2018.10.059

Cited by 5 publications

(5 citation statements)

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“…Among these, the thawing rate of cryopreserved samples is crucial to prevent processes like ice recrystallization and avoid osmotic stress during thawing (Henry et al, 1993). However, contemporary knowledge associates the optimal thawing rate with factors such as cell type, freezing rate, and composition of cryoprotective medium (Baboo et al, 2019). Since the thawing rate within the sample strongly depends on external temperature and container shape, the overall cryopreservation success requires the appropriate thawing conditions for specific freezing methods.…”

Section: Discussionmentioning

confidence: 99%

Optimizing aquaculture-scale common carp artificial reproduction: a novel approach to sperm cryopreservation using large-volume containers and elevated thawing temperatures

Sotnikov,

Rodina,

Gela

et al. 2024

Front. Mar. Sci.

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IntroductionThe successful cryopreservation of common carp sperm is crucial for its application in aquaculture and selective breeding programs. This study investigates the efficacy of cryopreserving sperm in large containers (5 mL) with a low dilution rate (1:1) in three different cryoprotective media and thawing in different conditions.MethodsThe developed method utilizes a low-ionic (hypotonic) cryoprotective medium, freezing with a controlled cooling rate, and high-temperature sperm thawing (60°C). The investigation employs a detailed spermatozoon motility assessment.ResultsPost-thaw motility of 32.3% ± 14% and initial curvilinear velocity of 89 ± 20 μm/s across 30 males were observed. Principal component analysis of sperm kinematic characteristics revealed distinct populations of sperm cells exhibiting varying responses to cryopreservation. The developed method achieved successful fertilization comparable to that of the non-frozen control group using sperm from a single cryotube (2.5 mL, approximately 50 * 109 spermatozoa) to fertilize 200 g of eggs (1:120,000 egg:spz).DiscussionThis novel approach demonstrates an effective cryopreservation protocol for common carp sperm in large-volume cryo-containers in combination with low-ionic cryomedia and high thawing temperature, providing methods well-suited for fisheries practices and selective breeding programs. Future studies of the biological properties of different sperm subpopulations in post-thaw sperm samples can contribute to a deeper understanding of sperm biology, improve cryopreservation techniques, and enhance the success rates of assisted reproductive technologies.

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Section: Discussionmentioning

confidence: 99%

Optimizing aquaculture-scale common carp artificial reproduction: a novel approach to sperm cryopreservation using large-volume containers and elevated thawing temperatures

Sotnikov,

Rodina,

Gela

et al. 2024

Front. Mar. Sci.

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“…Thawing time, washing medium, temperature, centrifugation time, and centrifugation force can all affect the recovery of cells after cryopreservation (Honge et al, 2017). Rapid thawing is generally believed to reduce the chance of damage caused by local re-freezing of cells (Baboo et al, 2019). However, there are few studies and patents focused on optimizing the recovery methods for DPSCs.…”

Section: Recovery Of Dpscsmentioning

confidence: 99%

Research progress on optimization of in vitro isolation, cultivation and preservation methods of dental pulp stem cells for clinical application

Wang,

Li,

et al. 2024

Front. Bioeng. Biotechnol.

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Due to high proliferative capacity, multipotent differentiation, immunomodulatory abilities, and lack of ethical concerns, dental pulp stem cells (DPSCs) are promising candidates for clinical application. Currently, clinical research on DPSCs is in its early stages. The reason for the failure to obtain clinically effective results may be problems with the production process of DPSCs. Due to the different preparation methods and reagent formulations of DPSCs, cell characteristics may be affected and lead to inconsistent experimental results. Preparation of clinical-grade DPSCs is far from ready. To achieve clinical application, it is essential to transit the manufacturing of stem cells from laboratory grade to clinical grade. This review compares and analyzes experimental data on optimizing the preparation methods of DPSCs from extraction to resuscitation, including research articles, invention patents and clinical trials. The advantages and disadvantages of various methods and potential clinical applications are discussed, and factors that could improve the quality of DPSCs for clinical application are proposed. The aim is to summarize the current manufacture of DPSCs in the establishment of a standardized, reliable, safe, and economic method for future preparation of clinical-grade cell products.

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“…Most cryopreservation protocols rely on slow warming in a hotwater bath which for 1 mL of cells in a 2 mL cryovial results in a warming rate of ~45 ± 8 °C/min (Baboo et al, 2019) (significantly slower than the cooling rates used in vitrification). This method of warming has two significant limitations: 1) the slow warming rate can lead to ice recrystallisation, especially for samples that have been vitrified (Sharma et al, 2021) and 2) there is an uneven temperature…”

Section: Heat Transfer Limitationsmentioning

confidence: 99%

Applying soft matter techniques to solve challenges in cryopreservation

Bryant

Elbourne

Greaves

et al. 2023

Front. Soft. Matter

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Cryopreservation allows the long-term storage of biological material, and has become integral for numerous applications including assisted reproductive technologies, stem cell therapies, blood banking, and species preservation. However, there are still hundreds of cell types that cannot be cryopreserved, and no organs at all. The biggest challenge facing cryopreservation is the ongoing reliance on predominantly just two cryoprotectants (dimethylsulfoxide and glycerol) as well as physical limitations to heating and cooling rates. The field of soft matter can accelerate cryopreservation research by providing insights into the underlying mechanisms and interactions of cells, cryoprotectants, and solvents including the role of temperature. With techniques as far ranging as differential scanning calorimetry, atomic force microscopy, and neutron and X-ray scattering, it is possible to probe multiple length and time scales in order to identify and characterise important interactions. This in turn can lead to the development of new cryoprotectants, and new methods of heating/cooling which could overcome some of the current challenges of cryopreservation.

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Interdependency of freezing and thawing rates on cryopreserved human t cells

Cited by 5 publications

References 0 publications

Optimizing aquaculture-scale common carp artificial reproduction: a novel approach to sperm cryopreservation using large-volume containers and elevated thawing temperatures

Optimizing aquaculture-scale common carp artificial reproduction: a novel approach to sperm cryopreservation using large-volume containers and elevated thawing temperatures

Research progress on optimization of in vitro isolation, cultivation and preservation methods of dental pulp stem cells for clinical application

Applying soft matter techniques to solve challenges in cryopreservation

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