Applying soft matter techniques to solve challenges in cryopreservation

Abstract

Cryopreservation allows the long-term storage of biological material, and has become integral for numerous applications including assisted reproductive technologies, stem cell therapies, blood banking, and species preservation. However, there are still hundreds of cell types that cannot be cryopreserved, and no organs at all. The biggest challenge facing cryopreservation is the ongoing reliance on predominantly just two cryoprotectants (dimethylsulfoxide and glycerol) as well as physical limitations to heating and cooling rates. The field of soft matter can accelerate cryopreservation research by providing insights into the underlying mechanisms and interactions of cells, cryoprotectants, and solvents including the role of temperature. With techniques as far ranging as differential scanning calorimetry, atomic force microscopy, and neutron and X-ray scattering, it is possible to probe multiple length and time scales in order to identify and characterise important interactions. This in turn can lead to the development of new cryoprotectants, and new methods of heating/cooling which could overcome some of the current challenges of cryopreservation.

Biophysical Characterization and Cryopreservation of Mammalian Cells Using Ionic Liquids

“…The permeability of THP-1 and HaCaT cells to EAA, EAL, EtAA, EAF, and ChA was assessed using optical microscopy and shrink-swell experiments. ,− 8-well chambered coverglass (Thermofisher) slides were prepared by coating with poly- l -lysine to ensure cell adhesion. Two hundred microliters of a cell suspension (∼(3–5) × 10 4 cells/mL) was added to a well and allowed to sit for 15 min to provide time for the cells to attach to the base of the well.…”