Interconnection between tricarboxylic acid cycle and energy generation in microbial fuel cell performed by desulfuromonas acetoxidans IMV B-7384

Abstract

Desulfuromonas acetoxidans IMV B-7384 is exoelectrogenic obligate anaerobic sulfur-reducing bacterium. Its one of the first described electrogenic bacterium that performs complete oxidation of an organic substrate with electron transfer directly to the electrode in microbial fuel cell (MFC). This bacterium is very promising for MFC development because of inexpensive cultivation medium, high survival rate and selective resistance to various heavy metal ions. The size of D. acetoxidans IMV B-7384 cells is comparatively small (0.4-0.8×1-2 μm) that is highly beneficial while application of porous anode material because of complete bacterial cover of an electrode area with further significant improvement of the effectiveness of its usage. The interconnection between functioning of reductive stage of tricarboxylic acid (TCA) cycle under anaerobic conditions, and MFC performance was established. Malic, pyruvic, fumaric and succinic acids in concentration 42 mM were separately added into the anode chamber of MFC as the redox agents. Application of malic acid caused the most stabile and the highest power generation in comparison with other investigated organic acids. Its maximum equaled 10.07±0.17mW/m 2 on 136 hour of bacterial cultivation. Under addition of pyruvic, succinic and fumaric acids into the anode chamber of MFC the maximal power values equaled 5.80±0.25 mW/m 2 ; 3.2±0.11 mW/m 2 , and 2.14±0.19 mW/m 2 respectively on 40, 56 and 32 hour of bacterial cultivation. Hence the malic acid conversion via reductive stage of TCA cycle is shown to be the most efficient process in terms of electricity generation by D. acetoxidans IMV B-7384 in MFC under anaerobic conditions.

Influence of potassium dichromate on the reduction of sulfur, nitrate and nitrite ions by bacteria Desulfuromonas sp.

“…The efficiency of electron acceptor reduction by bacteria is primarily determined by the difference between the oxidation-reduction potential of the electron donor and acceptor, which depends on the pH of the media and changes during cultivation of bacteria (Gescher & Kappler, 2012;Govorukha et al, 2015). Bacteria of the Desulfuromonas genus oxidize simple organic substrates completely to CO 2 and H 2 O in the tricarboxylic acid cycle or in the acetyl-CoA/CO-dehydrogenase pathway (Sung et al, 2003;An & Picarda, 2015;Vasyliv et al, 2015). Although the succession of electron acceptors' reduction by microorganisms at their simultaneous presence in the media is determined by their standard oxidation-reduction potential (pH 7.0), the energy supply of cells during anaerobic respiration depends on the ways of ATP synthesis in the process of electron donor oxidation: by substrate or oxidative phosphorylation (Lengeler et al, 2005;McKinlay et al, 2020).…”

“…can carry out sulfur respiration with the formation of hydrogen sulfide (Kozlova et al, 2008). Bacteria of the Desulfuromonas genus use elemental or polysulfide sulfur, nitrates, nitrites, L-malate, fumarate, tri-or tetrachlorethylene, oxidized forms of heavy metals (Cr(VI), Fe(III), Mn(IV), Cu(II)) as electron acceptors (Sung et al, 2003;Kuever et al, 2005;Moroz et al, 2014;An & Picarda, 2015;, oxidizing H 2 or the simple organic compounds to CO 2 (Roden & Lovley, 1993;Hedderich et al, 1999;Vasyliv et al, 2015). In Wolinella succinogenes polysulfide reduction occurs in the periplasm, using hydrogen and electron transport chain, which includes hydrogenase and polysulfide reductase.…”

Reduction of sulfur and oxidized forms of nitrogen by bacteria of Desulfuromonas sp., isolated from Yavorivske Lake, under the influence of ferrum citrate

“…Sulfur reducing bacteria of the Desulfuromonas genus are obligate anaerobes, they use elemental or polysulfide sulfur, nitrates, nitrites, Lmalate, fumarate, tri-or tetrachlorethylene, oxidized forms of heavy metals as electron acceptors (Hedderich et al, 1999;Sung et al, 2003;Kuever et al, 2005;An & Picarda, 2015), oxidizing at the same time a number of simple organic compounds to CO 2 (Maslovska & Hnatush, 2013;Vasyliv et al, 2015). Sulfidogenic bacteria attract the attention of researchers as potential agents for the purification of waters contaminated with hydrogen sulfide and heavy metals.…”

“…Soluble and insoluble metal compounds are reduced outside the cells by a system of membrane-bound metal reductases (multi-heme c-type cytochromes) (Gescher & Kappler, 2012;Richter et al, 2012;Breuer et al, 2015), therefore electrons are released into the medium, allowing these exoelectrogenic anaerobic bacteria to be used in the microbial fuel cells (MFC) as the high effective anode biocatalysts (Fitzgerald et al, 2013;Prokhorova et al, 2017;Simonte et al, 2017). Electric current generation by Desulfuromonas acetoxidans IMV B-7384 in a MFC was described (Bilyy et al, 2014;Vasyliv et al, 2015), and the interrelation between Fe(III) reduction and exoelectrogenesis performed by this bacteria in the MFC was established (Vasyliv et al, 2016). Metal ions or oxoanions can be reduced by microorganisms not only on the cell surface but also in the internal compartments, in the periplasm and cytoplasm (Richter et al, 2012).…”

Ovocidal action of glutaraldehyde and benzalkonium chloride mixture on Aonchotheca bovis (Nematoda, Capillariidae) embryogenesis

“…Sulfur-reducing bacteria are highly efficient biocatalysts of microbial anodic fuel cells, which provide the electric current generation during oxidation of organic compounds (Logan & Regan, 2006). Carbohydrates (glucose, sucrose, cellulose, starch), volatile fatty acids (formiate, acetate, butyrate), alcohols (ethanol, methanol), amino acids, proteins and even inorganic components (Alves et al, 2011;Richter et al, 2012;Vasyliv et al, 2015;Knoche et al, 2016) are used to generate the electric current in the microbial anodic fuel cells.…”