An Investigation of Embryo and Eggshell Development in
Nematodes of the GenusТrichuris (Nematoda, Trichuridae) Parasitizing Sheep in Central and South-Eastern Regions of Ukraine. Yevstafi eva, V. A., Yuskiv, I. D., Melnychuk, V. V., Yasnolob, I. O., Kovalenko, V. A., Horb, K. O. -Abundance and distribution of nematodes of the genus Тrichuris Schrank, 1788 parasitizing domestic sheep (Ovis aries Linnaeus, 1758) were studied in Poltava, Kyiv and Zaporizhzhia Regions of Ukraine. Th ree species of Тrichuris were found, Trichuris skrjabini Baskakov, 1924, Trichuris оvis Abildgaard, 1795 and Trichuris globulosa Linstow, 1901. Trichuris оvis and T. skrjabini were more common (54.9 and 35.7 %), whereas Т. globulosa was relatively rare (9.4 %) in the studied material. New species-specifi c and sex-related morphological characters and metric indices were reviewed as useful in better identifi cation of T. skrjabini, Т. оvis and Т. globulosa parasitizing sheep.
This study aimed to study the current state of the fauna of pathogens of nematodoses of the digestive tract of sheep in the Poltava region. The work was performed based on the Laboratory of the Department of Parasitology and Veterinary Sanitary Examination of Poltava State Agar University and in the conditions of sheep farms of various forms of ownership in the Poltava region. According to the conducted research, for the first time, a detailed description of the fauna of nematodes parasitizing in the gastrointestinal tract of sheep in the climatic conditions of the Poltava region is given. Generic and species identification of isolated helminths was performed. Indicators of intensity and invasions and abundance index of detected nematodoses of sheep were established. It was determined that the fauna of nematodes of the digestive tract of sheep is represented by 15 species of helminths belonging to 2 classes: Adenophorea and Secernentea, 5 series: Trichurida, Enoplida, Strongylida, Rhabditida, Oxyurida, and 12 genera: Trichuris, Haterongia, Haemonchus Trichostrongylus, Nematodirus, Aonchotheca, Oesophagostomum, Scriabinema, Chabertia, Strongyloides, Cooperia, Bunostomum. Detected nematodes are geohelminths that cause the following diseases in sheep: trichuriasis, haemonchosis, ostertagiosis, trichostrongylosis, nematodirosis, oesophagostomosis, chabertiosis, cooperosis, bunostomosis, scriabinemosis, capillariasis, and strongyloidosis. It was found that the total infestation of sheep by nematodes was 78.10 %, the intensity of the invasion ranged from 1 to 93 specimens, and the abundance index – from 0.01 to 21.39 samples. The most common in the study region were nematodes of four species: H. contortus (P – 60.81 %, II – 24.15 spec./head, AI – 14.69 spec.), O. circumcincta (P – 60.81 %, ІІ – 29.75 spec./head, AI – 18.09 spec.), T. colubriformis (P – 59.08 %, ІІ – 36.21 spec./head, AI – 21.39 spec.) and T. ovis (P – 55.04 %, II – 24.19 spec./head, AI – 13.31 spec.). Isolated nematodes of T. colubriformis and H. contortus are pathogens of zooanthroponoses and are dangerous to humans, indicating the study's relevance and importance. The obtained scientific data will increase the effectiveness of measures to control and prevent nematodoses of the gastrointestinal tract of sheep in farms of the Poltava region.
Today sheep breeding in the world is gaining important socio-economic significance. In the conditions of the Ukrainian market, the sector provides the population with food products, industry – raw materials for production. One of the criteria for the efficiency of the industry is the epizootic well-being of farms in relation to invasive diseases, especially the most common ones – nematodoses of the gastrointestinal tract of sheep, which cause areas of significant economic losses. One of the factors of transmission of invasive diseases are objects of the environment, contaminated with eggs and larvae of pathogens of parasitic diseases. Therefore, the disinvasion of these sites is considered one of the decisive measures in the fight against parasites, since the destruction of the invasive elements in the external environment leads to a break in the epizootic chain of parasitic diseases of sheep, including trichurosis. Due to the presence of species diversity of nematodes of the genus Trichuris, parasitizing in sheep, high resistance of their exogenous stages of development to the action of environmental factors and chemicals, it is important to study the disinvasive effect of modern chemical agents on eggs of trichurosis pathogens. The purpose of the work was to study in a laboratory the disinvasive properties of the means of domestic production “Virosan” in the case of eggs of nematodes of the genus Trichuris parasitizing in sheep. The results of the research revealed that Virosan (LTD “BioTestLab”, Ukraine), using its solutions in 0.5% and 1% concentrations for exposures of 10–60 minutes, exhibited pronounced disinvasive properties (DE – 91.80–100% respectively) in relation to the test culture of eggs nematodes of the genus Trichuris, isolated from sheep. The investigated drug causes destructive changes in the form of destruction of the egg shell on one side of the poles and the termination of the development of the embryo at various stages. It has been experimentally established that the use of the means for the test culture of eggs of trichuryses of sheep of different species has its own differences. High level of disinfestation efficiency is registered for the following modes of application of the product: for the species Trichuris globulosa – a solution of 0.25% concentration (exposure of 60 minutes), as well as 0.5% and 1.0% concentration (exposures 10, 30 and 60 minutes), DE is 93.48–100.00%; for the species T. skrjabini – a solution of 0.5% and 1.0% concentration for all proposed exposures DE is 92.31–100.00%; For the species T. ovis – for use of 0.5% and 1.0% of working solutions (exposure 30, 60 and 10–60 minutes) DE is 90.59–100.00%.
Hereby we provide the results of observation of clinical pathology of psoroptosis in rabbits and
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.