Interchangeable Uses of Autoradiographic and Peroxidase Markers for Electron Microscopic Detection of Neuronal Pathways and Transmitter-Related Antigens in Single Sections

Pickel, Virginia M.; Milner, Teresa A.

doi:10.1007/978-1-4757-2055-6_5

Cited by 8 publications

(2 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A number of dual-labeling methods are available for ultrastructural studies of synaptic interactions in the brain (Leranth and Pickel 1989;Pickel and Milner, 1989;van den Pol and Decavel, 1990;Smith and Bolam, 1992). The DABiNiDAB and the DAB/ BDHC technique employ two contrasting immunomarkers.…”

Section: Methodological Considerationsmentioning

confidence: 98%

Direct catecholaminergic-cholinergic interactions in the basal forebrain. I. Dopamine-?-hydroxylase- and tyrosine hydroxylase input to cholinergic neurons

Záborszky

Cullinan

1996

J. Comp. Neurol.

View full text Add to dashboard Cite

Immunocytochemical double-labeling techniques were used at the light and electron microscopic levels to investigate whether dopamine-beta-hydroxylase and tyrosine hydroxylase-containing axons contact basal forebrain cholinergic neurons. Dopamine-beta-hydroxylase- and tyrosine hydroxylase-positive fibers and terminals were found in close proximity to cholinergic neurons throughout extensive basal forebrain areas, including the vertical and horizontal limb of the diagonal band nuclei, the sublenticular substantia innominata, bed nucleus of the stria terminalis, ventral pallidum, and ventrolateral globus pallidus. Cholinergic cells in some aspects of the globus pallidus appeared to be contacted by tyrosine hydroxylase-positive but not dopamine-beta-hydroxylase-positive fibers, suggesting dopaminergic input to cholinergic neurons in these regions. Direct evidence for the termination of dopamine-beta-hydroxylase and tyrosine hydroxylase-positive fibers on cholinergic neurons was obtained in electron microscopic double-immunolabeling studies. Using high magnification light microscopic screening, both qualitative and quantitative differences were noted in the catecholaminergic innervation of forebrain cholinergic neurons. For example, while many cholinergic neurons were in close proximity to single dopamine-beta-hydroxylase-positive varicosities, others, particularly those located in the substantia innominatabed nucleus of the stria terminalis continuum, were apparently contacted by labeled fibers in repetitive fashion. The findings of the present study, together with our preliminary biochemical experiments (Zaborszky et al. [1993] Prog. Brain Res. 98:31-49) suggest that catecholaminergic afferents can differentially modulate forebrain cholinergic neurons. Such interactions may be important in learning and memory processes, and their perturbations may contribute to the cognitive decline seen in aging and in disorders such as Alzheimer's and Parkinson's diseases.

show abstract

Section: Methodological Considerationsmentioning

confidence: 98%

Direct catecholaminergic-cholinergic interactions in the basal forebrain. I. Dopamine-?-hydroxylase- and tyrosine hydroxylase input to cholinergic neurons

Záborszky

Cullinan

1996

J. Comp. Neurol.

View full text Add to dashboard Cite

show abstract

“…antibody (Pickel and Milner, 1989) and washed 6 times (5 minutes each time) in 0.02 M KPBS. To block nonspecific binding, the sections were incubated in a solution containing 10% normal goat serum (NGS) and 0.5% Triton X-100 in 0.02 M KPBS (pH 7.4) for 3 hours at room temperature.…”

Section: Calretinin Immunohistochemistrymentioning

confidence: 99%

Calretinin‐immunoreactive cells and fibers in the human amygdaloid complex

Sorvari

Pitkänen

1996

J. Comp. Neurol.

View full text Add to dashboard Cite

Calretinin is a calcium-binding protein that colocalizes with GABA in the cerebral cortex and hippocampus of the rat and the monkey. In the present study, we investigated the distribution of calretinin-immunoreactive cells and fibers in the human amygdaloid complex. A conspicuous feature was the high density of calretinin neurons in the human amygdala. The highest densities of the calretinin-immunoreactive neurons were observed in the anterior cortical nucleus, accessory basal nucleus, amygdalohippocampal area, and in the nucleus of the lateral olfactory tract. The paralaminar nucleus, central nucleus, medial nucleus, and the periamygdaloid cortex contained the lowest densities of calretinin neurons. In most of the amygdaloid areas, the calretinin cells had the appearance of aspiny or sparsely spiny local circuit neurons. However, in the amygdalohippocampal area, we found also densely spined dendrites. The cortical areas and the central nucleus were characterized by intense neuropil labeling, while the deep nuclei contained a high density of calretinin-immunoreactive fibers and terminals. Calretinin immunoreactivity was also found in the intra-amygdaloid fiber bundles, stria terminalis, and in the ventral amygdalofugal pathway. This suggests that in addition to the local circuit neurons, calretinin immunoreactivity is also located in neurons that connect the amygdaloid complex with the other brain areas. The distribution and morphological characteristics of calretinin-immunoreactive neurons differed from those of another calcium-binding protein, parvalbumin, in the human amygdala (Sorvari et al. [1995] J. Comp. Neurol. 360:185-212). This suggests that these two calcium-binding proteins are located in different populations of neurons.

show abstract

Prefrontal cortical efferents in the rat synapse on unlabeled neuronal targets of catecholamine terminals in the nucleus accumbens septi and on dopamine neurons in the ventral tegmental area

Sesack

Pickel

1992

J of Comparative Neurology

765

531

View full text Add to dashboard Cite

Physiological and pharmacological studies indicate that descending projections from the prefrontal cortex modulate dopaminergic transmission in the nucleus accumbens septi and ventral tegmental area. We investigated the ultrastructural bases for these interactions in rat by examining the synaptic associations between prefrontal cortical terminals labeled with anterograde markers (lesion-induced degeneration or transport of Phaseolus vulgaris leucoagglutinin; PHA-L) and neuronal processes containing immunoreactivity for the catecholamine synthesizing enzyme, tyrosine hydroxylase. Prefrontal cortical terminals in the nucleus accumbens and ventral tegmental area contained clear, round vesicles and formed primarily asymmetric synapses on spines or small dendrites. In the ventral tegmental area, these terminals also formed asymmetric synapses on large dendrites and a few symmetric axodendritic synapses. In the nucleus accumbens septi, degenerating prefrontal cortical terminals synapsed on spiny dendrites which received convergent input from terminals containing peroxidase immunoreactivity for tyrosine hydroxylase, or from unlabeled terminals. In single sections, some tyrosine hydroxylase-labeled terminals formed thin and punctate symmetric synapses with dendritic shafts, or the heads and necks of spines. Close appositions, but not axo-axonic synapses, were frequently observed between degenerating prefrontal cortical afferents and tyrosine hydroxylase-labeled or unlabeled terminals. In the ventral tegmental area, prefrontal cortical terminals labeled with immunoperoxidase for PHA-L were in synaptic contact with dendrites containing immunogold reaction product for tyrosine hydroxylase, or with unlabeled dendrites. These results suggest that: (1) catecholaminergic (mainly dopaminergic) and prefrontal cortical terminals in the nucleus accumbens septi dually synapse on common spiny neurons; and (2) dopaminergic neurons in the ventral tegmental area receive monosynaptic input from prefrontal cortical afferents. This study provides the first ultrastructural basis for multiple sites of cellular interaction between prefrontal cortical efferents and mesolimbic dopaminergic neurons.

show abstract

Interchangeable Uses of Autoradiographic and Peroxidase Markers for Electron Microscopic Detection of Neuronal Pathways and Transmitter-Related Antigens in Single Sections

Cited by 8 publications

References 73 publications

Direct catecholaminergic-cholinergic interactions in the basal forebrain. I. Dopamine-?-hydroxylase- and tyrosine hydroxylase input to cholinergic neurons

Direct catecholaminergic-cholinergic interactions in the basal forebrain. I. Dopamine-?-hydroxylase- and tyrosine hydroxylase input to cholinergic neurons

Calretinin‐immunoreactive cells and fibers in the human amygdaloid complex

Prefrontal cortical efferents in the rat synapse on unlabeled neuronal targets of catecholamine terminals in the nucleus accumbens septi and on dopamine neurons in the ventral tegmental area

Contact Info

Product

Resources

About