Intercellular adhesion molecule‐1 expression on the hepatocyte membrane of patients with chronic hepatitis B and C

Horiike, Norio; Onji, Morikazu; Kumon, Izumi; Kanaoka, M; Michitaka, Kojiro; Ohta, Yasuyuki

doi:10.1111/j.1600-0676.1993.tb00598.x

Cited by 23 publications

(1 citation statement)

References 14 publications

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“…ICAM-1 is overexpressed in sinusoidal endothelial cells in liver tissues with hepatitis. Hepatocytes at the site of inflammation express ICAM-1 (13,14). Recently developed enzymelinked immunosorbent assays (ELISA) for soluble forms of ICAM-1 (sICAM-1) and VCAM-1 (sVCAM-1) in serum are useful for monitoring chronic hepatitis and hepatocellular carcinoma (15,16).…”

Section: Introductionmentioning

confidence: 99%

Adhesive molecules and inflammatory markers among hepatitis C virus Saudi patients

Al-Jiffri

2017

Electron J Gen Med

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Background:Currently, about 2% of population are affected with hepatitis C worldwide, chronic hepatitis C (CHC) is the major cause of hepatic cirrhosis and referral for liver transplant. However, there is a high need for noninvasive methods for assessment of hepatocellular damage. Objective: The purpose of this study was to determine the strength of the association between adhesive molecules and inflammatory markers among hepatitis C virus Saudi patients. Methods: One hundred patients with chronic hepatitis C virus infection(64 males and 36 females, their age ranged from 28 to 53 years with circulating anti-HCV antibodies were equally categorized into two study groups: patients with CHC and patients with liver cirrhosis (LC). Also, one fifty healthy subjects were included as healthy controls. Serum alanine aminotransferase (ALT), soluble intercellular adhesion molecule1 (sICAM-1); Soluble vascular adhesion molecule 1(sVCAM-1); Soluble E-selectin(s-E-selectin) and Tumor necrosis factor-alpha (TNF-α) were assayed for all participants. Results: We observed elevation with regard to the healthy controls group in the parameters of ALT, sICAM-1, sVCAM-1, s-E-selectin and TNF-α for patients with CHC and patients with liver cirrhosis (LC). Also, a significant positive correlation between serum TNF-α, sICAM-1, sVCAM-1 and ALT values was detected. Conclusion:In conclusion, our results confirm that, in patients with chronic virus hepatitis and liver cirrhosis there is a significant positive correlation between serum TNF-α, sICAM-1, sVCAM-1 and ALT values. These findings suggest that serum TNF-α levels could be used as a sensitive predictor of liver inflammation, while serum ICAM-1 can be considered as a marker of hepatic necrosis and inflammatory activity in chronic hepatitis, while serum VCAM-1 is an indicator for the severity of liver cirrhosis.

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Section: Introductionmentioning

confidence: 99%

Adhesive molecules and inflammatory markers among hepatitis C virus Saudi patients

Al-Jiffri

2017

Electron J Gen Med

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Effect of tumor necrosis factor α and intercellular adhesion molecule-1 expression on immunogenicity of murine liver cells in mice

Bumgardner

Apte

et al. 1998

Hepatology

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Previously, we demonstrated that purified hepatocytes (in the absence of donor-type antigen-presenting cells) are immunogenic in vitro in mixed lymphocyte hepatocyte culture (MLHC) by virtue of expression of major histocompatibility complex (MHC) class I. 1-5 Tumor necrosis factor ␣ (TNF-␣) is an inflammatory cytokine known to up-regulate MHC class I and adhesion molecule expression, and has been demonstrated to be important in the rejection of vascularized organ allografts. [6][7][8] In some models, host treatment with a monoclonal antibody directed against this cytokine prolongs graft survival of rat cardiac, liver, and renal allografts. 6-12 However, the mechanism by which immunotherapy directed against this cytokine influences graft survival is unknown.Adhesion molecules (intercellular adhesion molecule-1 [ICAM-1] and vascular cell adhesion molecule-1 [VCAM-1]) also appear to play important roles in vascularized organ allograft rejection since monoclonal antibodies directed against ICAM-1 and VCAM-1 have been shown to be effective in prolonging graft survival of murine cardiac allografts, cynomologous monkey renal transplants, and human renal transplants. [13][14][15][16][17] ICAM-1 has been implicated in liver transplant rejection by studies that show increased levels of soluble ICAM-1 during rejection episodes and histological detection of increased ICAM-1 expression during liver allograft rejection. Parenchymal cells (including liver cells) may constitutively express or may be induced to express adhesion molecules by various cytokines. However, the role of adhesion molecules in the presentation of MHC antigens by parenchymal cells is not known. Cytokines, including interleukin-1, interferon gamma, and TNF-␣, have been shown to up-regulate hepatocyte expression of ICAM-1 and their adhesion to T lymphocytes in vitro. 18 However, the in vivo effects of various cytokines on adhesion molecule expression in the liver has not been described, and the consequences of in vivo cytokine exposure on the immunogenicity of liver cells has not been studied. The current studies were undertaken to determine the effect of the inflammatory cytokine Abbreviations: MLHC, mixed lymphocyte hepatocyte culture; MHC, major histocompatibility complex; TNF-␣, tumor necrosis factor ␣; ICAM-1, intercellular adhesion molecule-1; VCAM-1, vascular cell adhesion molecule-1; HC, hepatocyte; NPC, nonparenchymal cell; FBS, fetal bovine serum; MLNPC, mixed lymphocyte nonparenchymal cell culture; cpm, counts per minute; E:T, effector-to-target; GART-FITC, goat-anti-rat-fluorescein isothiocyanate conjugated; MFI, mean fluorescent intensity; allo-CTL, allospecific cytotoxic T lymphocyte; SMA, sponge matrix allograft.From the

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Efficacy of anti-intercellular adhesion molecule-1 immunotherapy on immune responses to allogeneic hepatocytes in mice

Bumgardner

Heininger

et al. 1998

Hepatology

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Adhesion molecules appear to play important roles in vascularized organ allograft rejection, because antibodies directed against them are effective in prolonging survival of vascularized organ allografts in rodents. However, the efficacy of these agents for cellular allografts is unknown. The current studies were undertaken to determine the role of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) on host immune responses to purified hepatocytes. Host mice (C3H, H-2 k ) grafted with hepatocytes in sponge matrix allografts (HC-SMA) received IgG isotype control, anti-ICAM-1, or anti-VCAM-1 monoclonal antibody (mAb) on days 0 through 9 after grafting. Twelve to 14 days later, host cells infiltrating the HC-SMA were assessed for the development of allospe- Adhesion molecules intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) are expressed on endothelial and some parenchymal cells. These adhesion molecules (ICAM-1 and VCAM-1) also appear to play important roles in vascularized organ allograft rejection, because antibodies directed against ICAM-1 and VCAM-1 have been shown to be effective in prolonging survival of vascularized murine cardiac allografts, cynomolgous monkey renal transplants, and human renal transplants, [1][2][3][4][5][6] as well as nonvascularized skin allografts. 7 ICAM-1 has been implicated in liver transplant rejection by studies that show increased levels of soluble ICAM-1 during rejection episodes and histological detection of increased ICAM-1 during liver allograft rejection. [8][9][10] Parenchymal cells may constitutively express or may be induced to express adhesion molecules by various cytokines. However, the role of adhesion molecules in the presentation of major histocompatibility complex (MHC) antigens by parenchymal cells is not known. Cytokines, including interleukin-1, interferon gamma, and tumor necrosis factor (TNF), have been shown to up-regulate hepatocyte expression of ICAM-1 and their adhesion to T lymphocytes in vitro. 11 However, the in vivo effects of various cytokines on adhesion molecule expression in the liver has not been described, and the consequences of in vivo cytokine exposure upon the immunogenicity of liver cells has not been reported.Previously, we demonstrated that purified hepatocytes in the absence of donor-type antigen-presenting cells are immunogenic in vitro in mixed lymphocyte hepatocyte culture as determined by their ability to stimulate proliferation and the development of allospecific cytolytic effector T cells (alloCTLs) in mixed lymphocyte hepatocyte culture. The observed immunogenicity was correlated with the expression of MHC class I antigens. 12 We have further demonstrated that purified hepatocytes are immunogenic in vivo and stimulate the development of allo-CTLs in sponge matrix allografts (SMA) bearing allogeneic hepatocytes. 13,14 In this report, we extend our previous in vivo studies using the hepatocytesponge matrix allograft (HC-SMA) model to examine the role of ICA...

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Intercellular adhesion molecule‐1 expression on the hepatocyte membrane of patients with chronic hepatitis B and C

Cited by 23 publications

References 14 publications

Adhesive molecules and inflammatory markers among hepatitis C virus Saudi patients

Adhesive molecules and inflammatory markers among hepatitis C virus Saudi patients

Effect of tumor necrosis factor α and intercellular adhesion molecule-1 expression on immunogenicity of murine liver cells in mice

Efficacy of anti-intercellular adhesion molecule-1 immunotherapy on immune responses to allogeneic hepatocytes in mice

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