“…Mindful of these conflicting results, we carried out careful experiments with different concentrations of bromo-octanoate or bromo-palmitate in order to determine if specific inhibition of diacylglycerol acyltransferase could be obtained. For phosphatidate phosphohydrolase, such inhibitors have been reported [19], and may prove of utility in testing whether this enzyme is as important in plants as it is in controlling TAG synthesis in animals [20]. At concentrations of 0.02-0.03 mM, we obtained 15-20% inhibition of the enzyme routinely, without any significant alteration in total lipid labelling.…”