Interactions of proteoliposomes from serogroup B Neisseria meningitidis with bone marrow-derived dendritic cells and macrophages: adjuvant effects and antigen delivery

Rodríguez, Tamara; Pérez, Oliver; Ménager, Nathalie; Ugrinović, Sanja; Bracho, Gustavo; Mastroeni, Pietro

doi:10.1016/j.vaccine.2004.07.049

Cited by 38 publications

(25 citation statements)

References 31 publications

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“…The recognition of these MAMPs by Toll-like receptors (TLRs) leads to activation of a complex system of signal transduction, leading to the maturation and activation of DCs. Previous studies by our group have demonstrated the potent DC activation by the AFPL1, as evidenced by a high production of proinflammatory cytokines (IL-1␤, IL-6, TNF, and IL-12) (Pérez et al 2001) and by the high expression in DC of co-stimulatory molecules CD40, CD80, and CD86 (Rodríguez et al 2005). MyD88-deficient mice immunized with AFCo1 and glycoprotein D of HSV-2 by i.n.…”

Section: Fig 1 Proteoliposome (Pl)-specific Antibody Responses Indumentioning

confidence: 82%

“…in the strategy elicited strong systemic and mucosal responses, with SIgA detected in saliva, faeces, and vaginal washes. Furthermore, it is known that AFPL1 can activate the maturation of dendritic cells (DC) and macrophages (Rodríguez et al 2005). Thus, the maturation of DC might have contributed to the strong mucosal responses detected.…”

Section: Fig 1 Proteoliposome (Pl)-specific Antibody Responses Indumentioning

confidence: 99%

“…Their potential as adjuvants has been tested with a variety of antigens: Plasmodium falciparum merozoite surface proteins 4 and 5 ), Herpes Simplex Virus type 2 (HSV-2) protein (Del Campo et al 2010), Streptococcus pyogenes proteins (Guilherme et al 2009), and purified Dermatophagoides siboney allergens (Lastre et al 2006). Both structures also induce crosspresentation and cytotoxic T-lymphocyte activity (Rodríguez et al 2005(Rodríguez et al , 2006. AFCo1 can be used mainly as a mucosal adjuvant because it is a stable particle with higher mucosal absorption ) and acid-resistant properties.…”

Section: Introductionmentioning

confidence: 99%

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Mucosal and systemic immune responses induced by a single time vaccination strategy in mice

et al. 2015

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Vaccination is considered by the World Health Organization as the most cost-effective strategy for controlling infectious diseases. In spite of great successes with vaccines, many infectious diseases are still leading killers, because of the inadequate coverage of many vaccines. Several factors have been responsible: number of doses, high vaccine reactogenicity, vaccine costs, vaccination policy, among others. Contradictorily, few vaccines are of single dose and even less of mucosal administration. However, more common infections occur via mucosa, where secretory immunoglobulin A plays an essential role. As an alternative, we proposed a novel protocol of vaccination called Single Time Vaccination Strategy (SinTimVaS) by immunizing 2 priming doses at the same time: one by mucosal route and the other by parenteral route. Here, the mucosal and systemic responses induced by Finlay adjuvants (AF Proteoliposome 1 and AF Cochleate 1) implementing SinTimVaS in BALB/c mice were evaluated. One intranasal dose of AF Cochleate 1 and an intramuscular dose of AF Proteoliposome 1 adsorbed onto aluminum hydroxide, with bovine serum albumin or tetanus toxoid as model antigens, administrated at the same time, induced potent specific mucosal and systemic immune responses. Also, we demonstrated that SinTimVaS using other mucosal routes like oral and sublingual, in combination with the subcutaneous route elicits immune responses. SinTimVaS, as a new immunization strategy, could increase vaccination coverage and reduce time-cost vaccines campaigns, adding the benefits of immune response in mucosa.

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Section: Fig 1 Proteoliposome (Pl)-specific Antibody Responses Indumentioning

confidence: 82%

Section: Fig 1 Proteoliposome (Pl)-specific Antibody Responses Indumentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mucosal and systemic immune responses induced by a single time vaccination strategy in mice

et al. 2015

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“…These components are well-characterized agonists of innate immunity receptors and are most likely responsible for the ability of PL to induce DC maturation (Rodrıguez et al 2005). Therefore, increased leukocyte stimulation and increase in spleen weight following three doses of the vaccine were not unexpected, considering that PL has potent immunostimulatory effects (Rodrıguez et al 2005; Bracho Animal studies evaluating potential toxicity of an allergen vaccine containing a LPS-derived monophosphoryl Lipid A (MPL) have described also remarked increases in host white blood cell levels and spleen weight (Baldrick et al 2002(Baldrick et al , 2004(Baldrick et al , 2007. Nevertheless, clinical trials of this MPL-containing allergen vaccine revealed a satisfactory safety profile for use in subcutaneous injection immunotherapy in patients suffering from grass pollen allergy (Rosewich et al 2010(Rosewich et al , 2013.…”

Section: Discussionmentioning

confidence: 99%

Safety of a proteoliposome fromNeisseria meningitidesas adjuvant for a house dust mite allergy vaccine

Ramírez

Bourg

Torralba

et al. 2017

Journal of Immunotoxicology

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The proteoliposome (PL) of Neisseria meningitidis serogroup B has been reported as a safe and potent vaccine adjuvant, inducing a T H 1-skewed response. The present study describes a pre-clinical safety evaluation of an allergy therapeutic vaccine candidate based on purified allergens from Dermatophagoides siboney house dust mite and PL as adjuvant, both components adsorbed onto aluminum hydroxide gel. Two separate studies of acute toxicity evaluation were performed in mice and rabbits, and two repeatdose studies were conducted in non-sensitized and allergen-sensitized Balb/c mice, respectively. The study in sensitized mice intends to model a therapeutic setting. Aerosolized allergen challenge was used in both settings to model natural respiratory exposure. In the therapeutic setting, mice were administered with three doses containing 2 lg allergen at weekly intervals [subcutaneous route] and subsequently challenged with aerosolized allergen for 6 consecutive days. Parameters of general toxicity effects were assessed via measures of behavior, body weight, food and water consumption, and macroscopic evaluation of organs. Histological examination of organs and the injection site was performed. Potential immunotoxicity effects at the systemic level were assessed by blood eosinophil counting and serum allergen specific IgE by ELISA The vaccine did not produce general or functional toxic effects of significance, at a dose up to 100 lg allergen per kg body weight. An expected local reaction at the injection site was observed, which could be attributed mostly to the immunological effect of aluminum hydroxide. The models implemented here suggest an acceptable safety profile of this vaccine for testing in clinical trials of allergy immunotherapy. ARTICLE HISTORY

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“…In accordance, proteoliposomes (PL), nanovesicles isolated from outer membranes Neisseria meningitidis serogroup B, have been used as effective vaccines in infants and adults; also the adjuvant properties of this molecules have been accurately described [2,3]. Exposure of Adjuvant Finlay proteoliposome (AFPL1) induces upregulation of MHC-II, MHC-I, CD40, CD80 and CD86 expression on the surface of murine bone marrow dendritic cells and macrophages [4]. In addition, proteoliposomes can be transformed into a microparticles (Adjuvant Finlay Cochleate 1, AFCo1), conserved the protective and adjuvants components [5].…”

Section: Introductionmentioning

confidence: 99%

Nasal immunization of mice with AFCo1 or AFPL1 plus capsular polysaccharide Vi from Salmonella typhi induces cellular response and memory B and T cell responses

Romeu

Lastre

Reyes

et al. 2014

Vaccine

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Interactions of proteoliposomes from serogroup B Neisseria meningitidis with bone marrow-derived dendritic cells and macrophages: adjuvant effects and antigen delivery

Cited by 38 publications

References 31 publications

Mucosal and systemic immune responses induced by a single time vaccination strategy in mice

Mucosal and systemic immune responses induced by a single time vaccination strategy in mice

Safety of a proteoliposome fromNeisseria meningitidesas adjuvant for a house dust mite allergy vaccine

Nasal immunization of mice with AFCo1 or AFPL1 plus capsular polysaccharide Vi from Salmonella typhi induces cellular response and memory B and T cell responses

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