Interactions of an anticancer drug lomustine with single and double stranded DNA at physiological conditions analyzed by electrochemical and spectroscopic methods

Temerk, Y. M.; Ibrahim, Mohamed; Ibrahim, Hossieny; Kotb, Mamdouh Ali

doi:10.1016/j.jelechem.2016.03.020

Cited by 34 publications

(18 citation statements)

References 41 publications

(39 reference statements)

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“…For a totally irreversible and an adsorption-controlled electrode process, the relationship between E p and scan rate n can be expressed by the Laviron's [23] equation: E p = E o À(RT)/(anF)ln(anF/RTk s )À(RT/anF)lnn, where a is the charge transfer coefficient, k s is the standard heterogeneous electron transfer rate constant, n is the number of electrons transferred, n is the scan rate, and E8 is the formal redox potential (other symbols have their usual meanings). Calculated standard heterogeneous elec- tron transfer rate constants were equal to 6.3 and 2.4 s À1 for LCT and LCT-dsDNA, respectively, and proven that dsDNA altered the electrochemical kinetics of LMT reduction [7,24]. As can be seen on Figure 6, no new voltammetric signals appeared after dsDNA-LCT interaction (the same situation was observed when LCT and LCT-dsDNA was examined with SWV).…”

Section: Voltammetric Studies Of Lct-dsdna Interactionsupporting

confidence: 58%

“…Electrochemical techniques offer inexpensive methods for environmental analysis [1][2][3]. There are several methods for the investigation of interaction between DNA and organic compounds [4][5][6][7]. Modern electrochemical techniques represent a very useful tool for this purpose [8], and mercury [9], amalgam [2], and carbon [10] electrodes are most often applied in such experiments.…”

Section: Introductionmentioning

confidence: 99%

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Lactofen – Electrochemical Sensing and Interaction with dsDNA

et al. 2017

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The electrochemical reduction of lactofen (LCT) at the glassy carbon (GCE) and silver amalgam film electrode (AMFE) is investigatedin detail by the means of square wave voltammetry (SWV), square wave stripping voltammetry (SWSV) and cyclic voltammetry. The influence of various factors such as supporting electrolyte composition and SW parameters were studied. The AMFE electrode showed an excellent electrochemical activity toward the electro-reduction of LCT, leading to a significant improvement in sensitivity as compared to the glassy carbon electrode.The SWSV detection limits for GCE and AMFE were 285.0 nM and 2.0 nM, respectively.The applicability of the developed voltammetric method for analysis of tap water and river water is illustrated with spiked samples analysis. Moreover, as lactofen is highly toxic to fish and other aquatic organisms, its interaction with dsDNA isolated from salmon sperm was tested. The intercalative mode of LCT binding to dsDNA was estimated. The heterogeneous rate constants were calculated for the free LCT and the LCT-dsDNA complex. Moreover, LCT-dsDNA complex binding ratio and equilibrium constant were determined. The decrease in the SWV peak current of LCT in the presence of dsDNA was used for the determination of dsDNA.

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Section: Voltammetric Studies Of Lct-dsdna Interactionsupporting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Lactofen – Electrochemical Sensing and Interaction with dsDNA

et al. 2017

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“…19,25 Spectroscopic techniques such as UV-vis absorption and fluorescence spectroscopy are being extensively employed in many studies to investigate changes in the physicochemical properties of small molecules in the presence of different biomolecules. 6,8,[26][27][28][29][30][31] These techniques are simple, accurate, highly sensitive, rapid, and convenient, and can often provide superior information compared with that obtained from many other complicated methods. Spectroscopic studies enable us to understand the modulations in the photophysical properties of the entrapped molecules, namely, fluorescence quantum yields, fluorescence lifetimes, radiative decay rates, non-radiative decay rates, etc.…”

Section: Introductionmentioning

confidence: 99%

Unraveling multiple binding modes of acridine orange to DNA using a multispectroscopic approach

Sayed

Krishnamurthy

Pal

2016

Phys. Chem. Chem. Phys.

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The interaction of acridine orange (AOH(+)) with calf thymus DNA (ct-DNA) under different dye-DNA conditions has been investigated in detail using multispectroscopic techniques, unraveling a number of hitherto unexplored intricacies of dye-DNA binding. The observed results intriguingly show contrasting binding features when low (2.4 μM) and significantly high (23 μM) dye concentrations are used. It is conclusively inferred from absorption, steady-state fluorescence, circular dichroism, fluorescence decay and anisotropy decay studies that at low [DNA] to [dye] ratio, especially with higher dye concentration, dimeric AOH(+) predominantly binds externally to DNA surfaces through electrostatic interactions. At sufficiently high [DNA] to [dye] ratios, however, the interaction intriguingly changes to monomeric AOH(+) bound to DNA, predominantly in the intercalative mode between DNA base pairs, with partly an electrostatic binding on DNA surfaces. With very low initial dye concentration, monomeric (AOH(+)) mostly binds to DNA through intercalative and electrostatic modes for most DNA to dye ratios. The present study demonstrates a systematic correlation of the striking changes in the photophysical properties of the dye upon multimode binding with DNA. The observed results are of great significance in understanding the fundamental insights of dye/drug binding to DNA hosts, of use in the design of effective therapeutic agents.

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“…In the presence of st‐DNA or ct‐DNA, the redox peak current decreases with a negative shift of the oxidation peak potential, which indicates the formation of slowly diffusing VLB‐DNA complex. The shift in the peak potential of CV to more negative or positive values is attributed to the intercalation of VLB into the DNA double‐helix . We assume this intercalation masks the electroactive site (dihydroxyl‐9,10‐anthraquinone moiety) of VLB which result in a decrease of the free VLB concentration.…”

Section: Vlb‐dna Interactionmentioning

confidence: 99%

A Novel Platform Based on Au−CeO₂@MWCNT Functionalized Glassy Carbon Microspheres for Voltammetric Sensing of Valrubicin as Bladder Anticancer Drug and its Interaction with DNA

Ibrahim

Almandil

et al. 2020

Electroanalysis

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In this work, a sensitive and simple electrochemical sensor was constructed for the quantitative analysis of valrubicin (VLB), as a chemotherapy drug, in biological samples based on MWCNTs-OH, CeO 2 NPs, AuNPs and functionalized glassy carbon microspheres (FGCM) modified electrode (AuÀ CeO 2 @MWCNT-OH/ FGCME). The new nanocomposite AuÀ CeO 2 @MWCNT-OH/FGCM produced an electrocatalytic effect towards the electrochemical oxidation of VLB. The electrochemical behavior of the AuÀ CeO 2 @MWCNT-OH/FGCME was also investigated using EIS and CV. The experimental and operation parameters that affect the sensitivity of the sensor were optimized. SWV was utilized for the electrochemical analysis of VLB. The oxidation peak currents were linearly dependent on the concentration of VLB in the range of 7.10 × 10 À 8 M to 5.8 × 10 À 7 M. The detection limit of VLB was found to be 1.56 × 10 À 9 M which was more sensitive than the reported method. The possible coexistence biological matrix had no interference effect on the determination of VLB. The fabricated sensor was also effectively employed to the sensitive detection of VLB in human body fluids. The interaction of VLB with salmon testes and calf thymus double-stranded DNA (st-DNA and ct-DNA) on AuÀ CeO 2 @MWCNT-OH/ FGCME has been further studied by cyclic voltammetry. The corresponding binding constant (K), surface concentration (Γ) and Gibbs free energy (ΔG°) were computed for the free VLB and the bound VLB-dsDNA complex. The obtained results revealed that VLB binds to DNA with a high binding constant as well as the K value for VLB-st-DNA (K = 1.81 × 10 5 M À 1) is higher than that of VLB-ct-DNA (K = 4.99 × 10 4 M À 1).

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Interactions of an anticancer drug lomustine with single and double stranded DNA at physiological conditions analyzed by electrochemical and spectroscopic methods

Cited by 34 publications

References 41 publications

Lactofen – Electrochemical Sensing and Interaction with dsDNA

Lactofen – Electrochemical Sensing and Interaction with dsDNA

Unraveling multiple binding modes of acridine orange to DNA using a multispectroscopic approach

A Novel Platform Based on Au−CeO₂@MWCNT Functionalized Glassy Carbon Microspheres for Voltammetric Sensing of Valrubicin as Bladder Anticancer Drug and its Interaction with DNA

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Interactions of an anticancer drug lomustine with single and double stranded DNA at physiological conditions analyzed by electrochemical and spectroscopic methods

Cited by 34 publications

References 41 publications

Lactofen – Electrochemical Sensing and Interaction with dsDNA

Lactofen – Electrochemical Sensing and Interaction with dsDNA

Unraveling multiple binding modes of acridine orange to DNA using a multispectroscopic approach

A Novel Platform Based on Au−CeO2@MWCNT Functionalized Glassy Carbon Microspheres for Voltammetric Sensing of Valrubicin as Bladder Anticancer Drug and its Interaction with DNA

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Product

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A Novel Platform Based on Au−CeO₂@MWCNT Functionalized Glassy Carbon Microspheres for Voltammetric Sensing of Valrubicin as Bladder Anticancer Drug and its Interaction with DNA