Lactofen – Electrochemical Sensing and Interaction with dsDNA

Abstract

The electrochemical reduction of lactofen (LCT) at the glassy carbon (GCE) and silver amalgam film electrode (AMFE) is investigatedin detail by the means of square wave voltammetry (SWV), square wave stripping voltammetry (SWSV) and cyclic voltammetry. The influence of various factors such as supporting electrolyte composition and SW parameters were studied. The AMFE electrode showed an excellent electrochemical activity toward the electro-reduction of LCT, leading to a significant improvement in sensitivity as compared to the glassy carbon electrode.The SWSV detection limits for GCE and AMFE were 285.0 nM and 2.0 nM, respectively.The applicability of the developed voltammetric method for analysis of tap water and river water is illustrated with spiked samples analysis. Moreover, as lactofen is highly toxic to fish and other aquatic organisms, its interaction with dsDNA isolated from salmon sperm was tested. The intercalative mode of LCT binding to dsDNA was estimated. The heterogeneous rate constants were calculated for the free LCT and the LCT-dsDNA complex. Moreover, LCT-dsDNA complex binding ratio and equilibrium constant were determined. The decrease in the SWV peak current of LCT in the presence of dsDNA was used for the determination of dsDNA.

Electrochemical and theoretical studies of the interaction between anticancer drug ponatinib and dsDNA

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Highly Sensitive Determination of Tenofovir in Pharmaceutical Formulations and Patients Urine—Comparative Electroanalytical Studies Using Different Sensing Methods

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Analysis and DNA interaction of the profluralin herbicide

“…Very small consumption of silver amalgam not exceeding 10 μL and long-term stability with more than two thousand measurement cycles (Baś 2006;Piech et al 2008a) make this electrode design environmentally friendly and consistent with green chemistry demands. Excellent properties of silver amalgam film electrode have been successfully utilized in the determinations of metals (Baś 2006;Piech et al 2008a, b), vitamins (Baś et al 2011), drugs (Smajdor et al 2015;Smarzewska et al 2017a, b), hormones (Smajdor et al 2016a(Smajdor et al , 2017, steroids (Smajdor et al 2016b) and pesticides (Smarzewska et al 2017c;Guziejewski et al 2017Guziejewski et al , 2018. Initial experiments revealed a distinct electrochemical activity of the profluralin and enabled its determination at the glassy carbon electrode (Smarzewska et al 2017c).…”