Interactions Between Oestradiol 3,17ß and Progesterone on the Induction and Growth of Deciduomata in Ovariectomized Mice

Abstract

SummaryThe hormonal control of uterine sensitivity in progesterone-treated, ovariectomized mice was investigated by stimulating the deciduoma reaction by various means. Uterine sensitivity reached a peak on day 5 of progesterone treatment and then declined. Oestradiol before and after stimulation significantly increased the weight of the deciduomata induced by crushing, bradykinin, and compound 48/80 and increased uterine sensitivity so that intraluminal peanut oil could induce deciduomata. A single injection of 0·024 fJ-g of oestradiol 8 hr before intraluminal peanut oil also greatly increased uterine receptivity to oil. The dose of oestradiol and the time of injection of oestradiol and progesterone were critical parameters. Surprisingly, the hormonal requirements for uterine sensitivity to intraluminal peanut oil are more stringent than for transferred blastocysts.Uterine sensitivity on day 10 could be increased by multiple injections of oestradiol before stimulation, but responses were not as large as those obtained following oil on day 5.From these data it is concluded that uterine sensitivity depends on the length of progestation, being maximal on day 5 of progesterone treatment, and on the amount of oestrogen before stimulation -uterine sensitivity may be increased by either a single or multiple doses of oestradiol.

Favorable influence of local injury to the endometrium in intracytoplasmic sperm injection patients with high-order implantation failure

“…Another form of trauma to the endometrium, intrauterine injections of oil, was reported in rats (12). Experiments in rats emphasized the possible involvement of histamine secreted by the uterus in response to trauma (13), and it was suggested that antihistamine treatment in rats may inhibit such a decidual response induced by a local injury (14).…”

The Effect of Local Injury to the Endometrium for Implantation and Pregnancy Rates in ICSI –ET Cycles with Implantation Failure: a randomised controlled study

“…In guinea pigs endometrial injury resulted in desidualization and improved reseptivity of the uterus to implantation (11). The same effect was observed by injecting oil into the endometrial cavity in mice (12). The injury induced desidualization could be prevented by administration of antihistamines into the uterine horn or by chronic treatment with chemical histamine releasers that produced depletion of endogenous histamine resources (13,14 The injury promotes decidualization of the endometrium making it more receptive for implantation.…”

“…Endometrial injury might provoke the wound healing, involving a massive secretion of different cytokines and growth factors, including leukemia inhibitory factor (LIF), interleukin-11 and heparin-banding endothelial growth factor-like growth factor (12)(13)(14). A high level of endometrial proinflammatory cytokines, such as interleukin-6, LIF, and Tumor Necrosis Factor-α (TNF-α), characterize early implantation (17).…”

Does adding endometrial scratching to diagnostic hysteroscopy improve pregnancy rates in women with recurrent in-vitro fertilization failure?

“…In 1907, endometrial scratching or induced injury of the endometrium in guinea pig showed to increase the possibility of implantation of embryo and endometrial decidualization [19,20]. Subsequently, endometrial injury by injection of oil to the endometrium in rodents is also resulted in histamine release from the uterine tissue and increased decidualization [21][22][23]. Intraperitoneal injection of histamine in rats also improved decidual response.…”