Interactions between nanoparticles and lung surfactant investigated by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry

Abstract

This work demonstrates that MALDI-TOFMS can be used for direct determination of the binding of surfactant lipids to nanoparticles and represents an important initial step towards a simple and quantitative in vitro method for assessment of interactions of nanoparticles with lung surfactants.

Effect of the Incorporation of Nanosized Titanium Dioxide on the Interfacial Properties of 1,2-Dipalmitoyl-sn-glycerol-3-phosphocholine Langmuir Monolayers

“…This confers an extraordinary strength to the TiO 2 NP−lipid bonds, 48,54,76,77 as a binding between DPPC and TiO 2 nanoparticles 4 times higher than that found for DPPC and silica nanoparticles is reported in the literature. 54 The inefficient respreading of the materials expelled from the interface to the subphase during the compression is associated with the appearance of hysteresis on the monolayer isotherm upon expansion. 78 Figure 3 shows, for the sake of an example, the isotherm of a complete compression−expansion cycle for one of the monolayers studied in this work.…”

“…The strong embedding of the TiO 2 into the lipid layer can be explained due to the complex interplay of multiple interactions including electrostatic attraction, hydrogen bonding, and direct coordination of the phosphate or carbonyl oxygen of the lipids with available titanium dioxide sites. This confers an extraordinary strength to the TiO 2 NP–lipid bonds, ,,, as a binding between DPPC and TiO 2 nanoparticles 4 times higher than that found for DPPC and silica nanoparticles is reported in the literature …”

“…The modification of the elasticity in the two phases is characteristic of TiO 2 since other types of particles do not affect the elasticity of the expanded liquid phase, e.g., hydrophilic and fumed silica or carbon black, altering only the elasticity corresponding to the condensed liquid ones, 30 which can be explained again considering the high affinity of lipids for TiO 2 surface. 54 Thus, the effect of TiO 2 on the LC phase is easily explainable considering that the nanoparticles strongly affect the reorientation of the lipid molecules at the interface. This reduces the packing degree of the lipids and consequently the cohesion of the monolayer.…”

“…In recent years, several studies have been devoted to the effect of nanoparticles of different natures on the physicochemical properties and structure of lipid monolayers. ,− These studies have shown the strong influence of the specific properties of the particles on their effects . Despite the extensive development of this research, the understanding of the effect of nanoparticles on the dynamic response has been neglected for a long time, even if it is probably a central aspect for exploring the adverse effects on the physiological response, ,− providing important information to complement previous studies on the interaction between TiO 2 and biologically relevant systems. − For the purpose of this study, we combine Langmuir trough experiments, equilibrium isotherms, and oscillatory barrier experiments with the morphological information obtained by Brewster angle microscopy (BAM). Thus, it is possible to establish correlations between the changes of the equilibrium and dynamic mechanical properties of the layer due to the nanoparticle incorporation with the changes in the molecular ordering and layer structure.…”

Inductively coupled plasma mass spectrometry based platforms for studies involving nanoparticle effects in biological samples

“…These dynamic transformations, in turn, affect the transport, mechanism, and toxicity of NPs. Current analytical methods yield limited insight into a subset of these transformations . In fact, few studies on NP transformations in biological samples exist, especially in plants because they represent only ca.…”