Interactions between dietary acrylamide intake and genes for ovarian cancer risk

Abstract

Some epidemiological studies observed a positive association between dietary acrylamide intake and ovarian cancer risk but the causality needs to be substantiated. By analyzing gene-acrylamide interactions for ovarian cancer risk for the first time, we aimed to contribute to this. The prospective Netherlands Cohort Study on diet and cancer includes 62,573 women, aged 55–69 years. At baseline in 1986, a random subcohort of 2589 women was sampled from the total cohort for a case cohort analysis approach. Dietary acrylamide intake of subcohort members and ovarian cancer cases (n = 252, based on 20.3 years of follow-up) was assessed with a food frequency questionnaire. We selected single nucleotide polymorphisms (SNPs) in genes in acrylamide metabolism and in genes involved in the possible mechanisms of acrylamide-induced carcinogenesis (effects on sex steroid systems, oxidative stress and DNA damage). Genotyping was done on DNA from toenails through Agena’s MassARRAY iPLEX platform. Multiplicative interaction between acrylamide intake and SNPs was assessed with Cox proportional hazards analysis. Among the results for 57 SNPs and 2 gene deletions, there were no statistically significant interactions between acrylamide and gene variants after adjustment for multiple testing. However, there were several nominally statistically significant interactions between acrylamide intake and SNPs in the HSD3B1/B2 gene cluster: (rs4659175 (p interaction = 0.04), rs10923823 (p interaction = 0.06) and its proxy rs7546652 (p interaction = 0.05), rs1047303 (p interaction = 0.005), and rs6428830 (p interaction = 0.05). Although in need of confirmation, results of this study suggest that acrylamide may cause ovarian cancer through effects on sex hormones.Electronic supplementary materialThe online version of this article (doi:10.1007/s10654-017-0244-0) contains supplementary material, which is available to authorized users.

Dietary Intake of Acrylamide and Risk of Breast, Endometrial, and Ovarian Cancers: A Systematic Review and Dose–Response Meta-analysis

“…We reported general characteristics of included studies in Table 1. The most common study designs were the cohort (eleven studies) (30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40) and case-cohort (five studies) (7,(41)(42)(43)(44), followed by two case-control studies (28,29). These studies were published between 2005 and 2019, and the vast majority were carried out in Europe (N=15), followed by the US (N=2) and Japan (N=1).…”

“…Some studies assessed more than one cancer type. Overall, ten studies assessed breast cancer (28,(30)(31)(32)(33)(34)(35)42,44), seven endometrial cancer (7,29,(35)(36)(37)40,43), and seven ovarian cancer (7,28,35,36,38,39,41), with a total of 18,100, 3561, and 3569 cases of breast, endometrial, and ovarian cancer, respectively.…”

“…Acrylamide dietary intake ranged from 3.6 µg/day to 44 µg/day, with both mean and median values of 21 µg/day (range 6.3 to 29.8 µg/day). All but three studies reported risk estimates among neversmoking women (28,33,39), while six carried out stratified analysis according to menopausal status (29)(30)(31)(35)(36)(37) or recruited either pre- (34) or postmenopausal women only (7,32,40,41,43). Finally, five studies presented results stratified by body mass index (i.e.…”

“…estrogen receptor-ER status and/or progesterone receptor-PR status ( Supplemental Table S1 (31,32,34,35,42,44). Five reports were available on the same study population (7,(41)(42)(43)(44), and in these cases we included only the most recent and complete papers in our analyses (41)(42)(43).…”

Nutrition, Cancer Genetics and Epigenetics

“…Therefore, dietary recommendations should consider individual genotype differences while applying nutritional genomics for personalized preventive and therapeutic intervention in colon cancer patients. In another approach, the potential association between dietary acrylamide intake and the genes related to ovarian cancer risk was addressed [69]. A study conducted with 62,573 women, concerning the genes involved in acrylamide metabolism and acrylamide-induced carcinogenesis, revealed 57 SNPs and two gene deletions, although no statistically significant interactions between acrylamide and the gene variants were observed.…”