Interactions between accumulated copper, bacterial community structure and histamine levels in crayfish meat during storage

Abstract

Copper from the habitat appears to reduce histamine accumulation in crayfish meat during storage by affecting the bacterial community structure of the cultivation water and most likely also in the intestine of the crayfish. From a food safety point of view, copper treatment during the aqua culturing of crustaceans has a positive impact on the postharvest stage.

Role of Marine Bacterial Contaminants in Histamine Formation in Seafood Products: A Review

“…Marine bacteria with the capability to produce HDC are a serious concern in seafood industries as they can accumulate histamine in seafood products through the conversion of free amino acid histidine into histamine [50,51]. In the family of Crustaceae, this biogenic amine has been reported to be produced naturally along with the ecology of the associated bacteria, and it acts as a neurotransmitter [52]. HDC producing marine bacteria commonly found in seafood products include both Gram-positive and Gram-negative bacteria.…”

“…These bacteria play a significant role in the deterioration of this seafood product and lead to the formation of biogenic amines during storage. The accumulation of histamine in this product to levels exceeding the safety limit [52] will be dependent on the bacterial community composition, storage time, and incubation temperature.…”

“…However, Pseudomonas species (P. fluorescens, P. putida, and non-fluorescent Pseudomonas spp.) contribute to the reduced histamine levels [52].…”

“…The identification of isolates with the capability to produce histamine was achieved by amplification followed by the sequencing of approximately 1400 bp of their 16S ribosomal DNA (rDNA) or 16sRNA [17,52,60]. In this amplification, the hdc gene of the Gramnegative bacteria is the target for amplification using primers of hdc-f and hdc-r with the capability to amplify a DNA fragment of 709 base pairs.…”

“…Meanwhile, the hdc gene of Gram-positive bacteria is usually amplified using HDC-3 and HDC-4 primers. These primers could amplify the DNA fragment of 435 bp [52]. The gene of hdcA (the internal parts of hdc gene cluster) is commonly detected using two sets of different primers (Hdc1/Hdc2 and JV16HC/JV17HC) in the amplification of such genes [5].…”