Interaction study between double-stranded DNA and berberine using capillary zone electrophoresis

Wu, Juanfang; Chen, Lingxin; Luo, Gang; Liu, Yong Suo; Wang, Yi Ming; Jiang, Zhi Hong

doi:10.1016/j.jchromb.2006.01.028

Cited by 8 publications

(3 citation statements)

References 38 publications

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“…Alternatively, capillary gel electrophoresis (CGE) separates nucleic acids by their size and conformation (smaller analytes migrate faster than longer ones) by sieving through a gel. ,, Denaturing conditions are favored when the separation efficiency is important, because they allow the separation of ssDNA with increased resolutions compared to the corresponding dsDNA molecules. , Nondenaturing conditions result in mobility shifts for changes as small as a single nucleotide in ssDNA (but not dsDNA), due to conformational changes . Nondenaturing CE also allows separating DNA complexed by small molecules or proteins and estimating binding constants and thermodynamic parameters of complex formation. − …”

Section: Mass Spectrometry Methodsmentioning

confidence: 99%

“…566 Nondenaturing CE also allows separating DNA complexed by small molecules or proteins, and estimating binding constants and thermodynamic parameters of complex formation. [575][576][577][578][579][580] Combining the advantages of CE to MS (mostly ESI, but MALDI and ICP were also used) is attractive but remains challenging, in particular the coupling of the separation voltage with that of the MS source, and the significant dilution of the CE effluent in co-axial sheath liquid interfaces. 571,581 Compared to IP-RP-LC, the low flow rates of CE and the absence of ion-pairing agents are beneficial to improve MS ionization.…”

Section: Non-denaturing Capillary Electrophoresis Couplingmentioning

confidence: 99%

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Mass Spectrometry of Nucleic Acid Noncovalent Complexes

et al. 2021

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Nucleic acids have been among the first targets for antitumor drugs and antibiotics, and with the unveiling of new biological roles in regulation of gene expression, specific DNA and RNA structures have become very attractive targets, especially when the corresponding proteins are undruggable. Biophysical assays to test target structure and ligand binding stoichiometry, affinity, specificity and binding modes are part of the drug development pipeline. Mass spectrometry offers unique advantages as a biophysical method due to its ability to distinguish each stoichiometry present in a mixture. In addition, advanced mass spectrometry approaches (reactive probing, fragmentation techniques, ion mobility spectrometry, ion spectroscopy) provide more detailed information on the complexes. Here we review the fundamentals of mass spectrometry and all its particularities when studying non-covalent nucleic acid structures, and then review what has been learned thanks to mass spectrometry on nucleic acid structures, self-assemblies (e.g., duplexes or G-quadruplexes), and their complexes with ligands. 47

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Section: Mass Spectrometry Methodsmentioning

confidence: 99%

Section: Non-denaturing Capillary Electrophoresis Couplingmentioning

confidence: 99%

Mass Spectrometry of Nucleic Acid Noncovalent Complexes

et al. 2021

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“…In another study devoted to berberine, the authors suggested the use of CE to study the specificity of the sequence of interaction with the DNA. Taking Hoechst 33258 as a positive control, the authors found a weak‐binding preference for the AATT‐containing rather than the GGCC‐containing dsDNA (Wu et al ., ).…”

Section: Applicationmentioning

confidence: 97%

The advances of electromigration techniques applied for alkaloid analysis

Dziomba

Belka

Kowalski

et al. 2013

Biomedical Chromatography

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Alkaloids are natural products of metabolism found mainly in plants. Their diversified pharmacological activities as medical agents and extensive occurrence in nutritional products demand the development of reliable and sensitive analytical tools. This review presents the developments in the field of electromigration techniques and specifically capillary and microchip electrophoresis. We include the main aspects of interest to researches over the past 12 years. The scope of this review covers detection, on- and off-line preconcentration techniques, chiral separation and developments in the field of microchip electrophoresis. Their applications in alkaloid determination, capillary electrochromatography and inter-molecular interactions are also examined.

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Nanostructured electrochemical DNA biosensors for detection of the effect of berberine on DNA from cancer cells

et al. 2006

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Multi walled carbon nanotubes (MWNT) in dimethylformamide (DMF) or aqueous sodium dodecyl sulfate (SDS) solution, colloidal gold nanoparticles (GNP) in phosphate buffer solution (PBS), and a GNP-MWNT mixture in aqueous SDS solution have been investigated for chemical modification of a screen-printed carbon electrode used as the signal transducer of a dsDNA-based biosensor. Differential pulse voltammetry of the DNA redox marker Co[(phen)3]3+ and the guanine moiety anodic oxidation and cyclic voltammetry with K3[Fe(CN)6] as indicator revealed substantial enhancement of the response of the biosensor, particularly when MWNT in SDS solution was used. The biosensor was used in testing of berberine, an isoquinoline plant alkaloid with significant antimicrobial and anticancer activity. Berberine had a very strong, concentration-dependent, effect on the structural stability of DNA from the human cancer cells (U937 cells) whereas non-cancer cells were changed only when berberine concentrations were relatively high 75 and 50 microg mL(-1).

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Interaction study between double-stranded DNA and berberine using capillary zone electrophoresis

Cited by 8 publications

References 38 publications

Mass Spectrometry of Nucleic Acid Noncovalent Complexes

Mass Spectrometry of Nucleic Acid Noncovalent Complexes

The advances of electromigration techniques applied for alkaloid analysis

Nanostructured electrochemical DNA biosensors for detection of the effect of berberine on DNA from cancer cells

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