Interaction peculiarities of 5,10,15,20-tetrakis(4-N-methylpyridil) tetra iodide porphyrin with albumin

Lebedevа, N. Sh.; Mal’kova, E. A.; Попова, Т. Е.; Kutyrev, A.E.; Сырбу, С. А.; Parfenyuk, Elena V.; V’yugin, A. I.

doi:10.1016/j.saa.2013.06.101

Cited by 10 publications

(3 citation statements)

References 6 publications

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“…The stability of the lipid bilayer can be decreased by interaction with cationic porphyrins [26] which can be beneficial to drug absorption. Porphyrin-BSA complexes also have higher stability [28]. In this study, DhHP-6 with a cationic porphyrin ring was more stable in SGF, SIF, and plasma.…”

Section: Discussionmentioning

confidence: 56%

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Oral DhHP-6 for the Treatment of Type 2 Diabetes Mellitus

Wang

Liang

et al. 2019

IJMS

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Type 2 diabetes mellitus (T2DM) is associated with pancreatic β-cell dysfunction which can be induced by oxidative stress. Deuterohemin-βAla-His-Thr-Val-Glu-Lys (DhHP-6) is a microperoxidase mimetic that can scavenge reactive oxygen species (ROS) in vivo. In our previous studies, we demonstrated an increased stability of linear peptides upon their covalent attachment to porphyrins. In this study, we assessed the utility of DhHP-6 as an oral anti-diabetic drug in vitro and in vivo. DhHP-6 showed high resistance to proteolytic degradation in vitro and in vivo. The degraded DhHP-6 product in gastrointestinal (GI) fluid retained the enzymatic activity of DhHP-6, but displayed a higher permeability coefficient. DhHP-6 protected against the cell damage induced by H2O2 and promoted insulin secretion in INS-1 cells. In the T2DM model, DhHP-6 reduced blood glucose levels and facilitated the recovery of blood lipid disorders. DhHP-6 also mitigated both insulin resistance and glucose tolerance. Most importantly, DhHP-6 promoted the recovery of damaged pancreas islets. These findings suggest that DhHP-6 in physiological environments has high stability against enzymatic degradation and maintains enzymatic activity. As DhHP-6 lowered the fasting blood glucose levels of T2DM mice, it thus represents a promising candidate for oral administration and clinical therapy.

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Section: Discussionmentioning

confidence: 56%

“…Quadraplexes can be stabilized by porphyrins with a high number of charges [27]. Porphyrins can form stable complexes with bovine serum albumin (BSA) [28], and compounds with cationic porphyrins, such as DhHP-6, can become more stable and penetrate membrane bilayers more easily.…”

Section: Introductionmentioning

confidence: 99%

Oral DhHP-6 for the Treatment of Type 2 Diabetes Mellitus

Wang

Liang

et al. 2019

IJMS

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“…These spectral changes are caused by quenching of the excited state of porphyrin due to π-π binding to nitrogenous bases of the DNA duplex, and the inversion of fluorescence bands, according to the literature, is explained by a change in the state of pyrrole protons of the porphyrin reaction center (shift of tautomeric equilibrium). [19] The fluorescence quenching of porphyrins with meta-isomeric peripheral substituents manifests itself differently. First, the position of the fluorescence maximum in the far-wavelength part of the spectrum changes, it shifts from 702 nm to 713 nm, while the position of the fluorescence maximum in the region of 647 nm is retained.…”

Section: Resultsmentioning

confidence: 99%

Spectral Сharacterization of Сomplexes of Tetra- and Tricationic Porphyrins with DNA Duplex

Лебедева¹,

Yurina²,

Lebedev³

et al. 2021

MHC

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A systematic spectral study of the interaction of DNA with a number of tetra-and tricationic porphyrins, in which the N-methyl group is located in the para-or meta-position of the pyridyl substituent, has been carried out. The conditions for the formation of intercalation complexes of DNA with the studied porphyrins were established. It was shown that DNA exhibits greater affinity for porphyrins with an N-methyl group in the para-position of the peripheral substituent, compared to porphyrins with an N-methyl group in the meta-position. Intercalation complexes of DNA with porphyrins with the meta-position of the N-methyl group are characterized by spectral features, such as a slight bathochromic shift of the Soret band in the UV-Visible spectrum and the absence of band inversion in the fluorescence spectrum of intercalated porphyrins. For DNA complexes with monohetaryl-substituted porphyrins, a "semi-intercalation" binding model has been proposed.

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Salicylaldimine‐bridged dinuclear cyclopalladated complexes: Synthesis, characterization and BSA binding studies

Gao

Zhang

et al. 2019

Applied Organom Chemis

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Salicylaldimine‐bridged dinuclear cyclopalladated complexes were synthesized by the reactions of cyclopalladated chloro dimers [Pd{(4‐R)C6H3CH=N‐C6H3–2,6‐i‐Pr2}(μ‐Cl)]2 (R = H; OMe) with salen‐based bridging ligands. The complexes were characterized by FTIR, NMR spectroscopy, elemental analysis and X‐ray crystallography. The binding interaction of cyclopalladated complexes to bovine serum albumin (BSA) was investigated by UV–vis, fluorescence and synchronous fluorescence spectroscopy. The experimental results showed that these Pd (II) complexes could bind to BSA with high affinity and quench its intrinsic fluorescence by a static or combined process. Also the interaction of Pd complexes with BSA affected the conformation of the tryptophan and tyrosine residues.

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Interaction peculiarities of 5,10,15,20-tetrakis(4-N-methylpyridil) tetra iodide porphyrin with albumin

Cited by 10 publications

References 6 publications

Oral DhHP-6 for the Treatment of Type 2 Diabetes Mellitus

Oral DhHP-6 for the Treatment of Type 2 Diabetes Mellitus

Spectral Сharacterization of Сomplexes of Tetra- and Tricationic Porphyrins with DNA Duplex

Salicylaldimine‐bridged dinuclear cyclopalladated complexes: Synthesis, characterization and BSA binding studies

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