Interaction of α-synuclein with Rhus typhina tannin – Implication for Parkinson’s disease

Abstract

The etiology of Parkinson's disease (PD) relates to α-synuclein, a small protein with the ability to aggregate and form Lewy bodies. One of its prevention strategies is inhibition of α-synuclein oligomerization. We have investigated the interaction of α-synuclein and human serum albumin with 3,6-bis-О-di-О-galloyl-1,2,4-tri-О-galloyl-β-d-glucose (a tannin isolated from the plant Rhus typhina). Using fluorescence spectroscopy method we found that this tannin interacts strongly with α-synuclein forming complexes. Circular dichroism analysis showed a time-dependent inhibition of α-synuclein aggregation in the presence of the tannin. On the other hand, 3,6-bis-О-di-О-galloyl-1,2,4-tri-О-galloyl-β-d-glucose had a much stronger interaction with human serum albumin than α-synuclein. The calculated binding constant for tannin-protein interaction was considerably higher for albumin than α-synuclein. This tannin interacted with albumin through a "sphere of action" mechanism. The results lead to the conclusion that 3,6-bis-О-di-О-galloyl-1,2,4-tri-О-galloyl-β-d-glucose is a potent preventive compound against Parkinson's disease. However, this tannin interacts very strongly with human serum albumin, significantly reducing the bioavailability of this compound.

Influence of valoneoyl groups on the interactions between Euphorbia tannins and human serum albumin

“…12.4 ± 1.1 × 10 13 M −1 s −1 for T1, T2 and T3, respectively. If the k q value is larger than 2 × 10 10 M −1 s −1 (value of the maximum scatter collision-quenching constant) [25], then quenching occurs by a static mechanism and according to our data this mechanism of interaction of our tannins with albumin takes place. Next, the number of binding sites (n) in HSA and the binding constant (K b ) were calculated using the Hill equation (Eq.…”

“…Inhibition of the formation of advanced glycation endproducts (AGEs) by PGG is connected with protecting protein structure [23]. Tannins can act as anti-aggregation agents that inhibit the formation of Aβ1-42 fibrils [24] and α-synuclein oligomerization [25].…”

Gastroprotective and anti-inflammatory activities integrated to chemical composition of Myracrodruon urundeuva Allemão - A conservationist proposal for the species

“…It was observed, in the present study, the occurrence of flavonoids and tannins, as described in Table 1. The relevant biological activity of tannins is due to the interaction of these compounds with a variety of molecules, especially proteins (Barrett et al, 2017;Sekowski et al, 2017). This, added to the antioxidant properties of tannins (Karas et al, 2017;Deng et al, 2016) and anti-inflammatory (Spinaci et al, 2018;Souza et al, 2007) explain, at least in part, the pharmacological actions of these compounds.…”

Hippophae rhamnoides L. leaf and twig extracts as rich sources of nutrients and bioactive compounds with antioxidant activity

“…Spectra were obtained between 260 and 195 nm with a 0.5 cm path length Helma quartz cell. The recording parameters were as follows: scan speed, 50 nm/min; step resolution, 0.5 nm; response time, 4 s.; band width, 1 nm, and slit auto 63 , 64 . The CD spectra were corrected against the baseline with buffer only.…”