Interaction of water-soluble pentaamino acid fullerene derivatives with membranes of phosphatidylcholine liposomes

Kotelnikova, R. A.; Smolina, A. V.; Zhilenkov, Alexander V.; Soldatova, Yu. V.; Faingold, I. I.; Troshin, Pavel A.; Kotelnikov, A. R.

doi:10.1007/s11172-018-2082-y

Cited by 11 publications

(5 citation statements)

References 3 publications

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“…Detection of singlet oxygen 1 O 2 formation has been performed by a standard method using 1,3-diphenylisobenzofuran (DPBF) [ 31 ]. To introduce a hydrophobic probe DPBF to an aqueous solution, it was incorporated into the phosphatidylcholine liposomes using a modified method [ 32 , 33 ]. The aqueous solution containing DPBF (0.05 mM of DPBF in liposomes, 0.5·mM of lecithin) and the photosensitizer (5 μM), in a 10 × 10 mm quartz cuvette was irradiated with the setup, as described above.…”

Section: Methodsmentioning

confidence: 99%

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Nanoparticles of N-Vinylpyrrolidone Amphiphilic Copolymers and Pheophorbide a as Promising Photosensitizers for Photodynamic Therapy: Design, Properties and In Vitro Phototoxic Activity

et al. 2023

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A series of nanoparticles (NPs) with a hydrodynamic radius from 20 to 100 nm in PBS was developed over the solubilization of hydrophobic dye methyl pheophorbide a (chlorin e6 derivative) by amphiphilic copolymers of N-vinylpyrrolidone with (di)methacrylates. Photophysical properties and biological activity of the NPs aqueous solution were studied. It was found that the dye encapsulated in the copolymers is in an aggregated state. However, its aggregation degree decreases sharply, and singlet oxygen quantum yield and the fluorescence signal increase upon the interaction of these NPs with model biological membranes—liposomes or components of a tissue homogenate. The phototoxic effect of NPs in HeLa cells exceeds by 1.5–2 times that of the reference dye chlorin e6 trisodium salt—one of the most effective photosensitizers used in clinical practice. It could be explained by the effective release of the hydrophobic photosensitizer from the NPs into biological structures. The demonstrated approach can be used not only for the encapsulation of hydrophobic photosensitizers for PDT but also for other drugs, and N-vinylpyrrolidone amphiphilic copolymers show promising potential as a modern platform for the design of targeted delivery vehicles.

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Section: Methodsmentioning

confidence: 99%

“…Preparation of liposomes solution. Phosphatidylcholine (lecithin) from egg yolk (Sigma) was used to prepare liposomes’ solution according to the method described in [ 32 , 33 ]; lipid concentration in the cuvette was 100 μM.…”

Section: Methodsmentioning

confidence: 99%

Nanoparticles of N-Vinylpyrrolidone Amphiphilic Copolymers and Pheophorbide a as Promising Photosensitizers for Photodynamic Therapy: Design, Properties and In Vitro Phototoxic Activity

et al. 2023

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“…Because of this, a variety of fullerene-based compounds having attractive biological activities have been synthesized for anticancer or antimicrobial therapy, enzyme inhibition, cytoprotection, controlled drug delivery, and radioactivity-based diagnosis. [5][6][7][8][9][10][11] One promising research in this field is how to make fullerene soluble not only in chemical solvents but also in water. However, it is still challenging to disperse fullerenes because of its high crystallinity.…”

Section: Introductionmentioning

confidence: 99%

Fullerene Nanoparticles Using Technol PG for Inexpensive Preparation

Uchida

Yanagi

Hamada

2022

Natural Product Communications

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“…Many fullerene-based compounds with different biological activities have been synthesized for either antimicrobial or anticancer therapy, cytoprotection, enzyme inhibition, contrast- or radioactivity-based diagnosis, and controlled drug delivery. 4-11 Although ways to disperse fullerenes in water play an important role in their application in biomaterials, it is still a challenge to disperse them due to their high crystallinity. Among dispersants, phospholipids, surfactants derived from biomolecules, are promising, and the preparation of fullerene nanoparticles using DPPC, a neutral phospholipid, has been reported.…”

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confidence: 99%