Interaction of vanadium(IV) with human serum apo-transferrin

Vanadium and proteins: Uptake, transport, structure, activity and function

“…Binding of V IV O compounds to hTF may have an important role in the transport and delivery of vanadium based drugs [26,30,84,88,97,117,118,119,120,121,122]. the formation of cis-V IV OL 2 (holo-hTF) is possible through the replacement of the equatorial water molecule by an accessible His-N in a Type 1 binding (Fig.…”

A novel VIVO–pyrimidinone complex: synthesis, solution speciation and human serum protein binding

“…whether ternary or quaternary V IV O-complexes form, which hTF donor groups/residues bind to the metal centre and if hTF can assume (or not) the closed conformation recognizable by the transferrin receptor in the presence of mhcpe ( probably yes). 57 Interaction of vanadium-mhcpe complexes with transferrin in the presence of iron As mentioned above, in normal serum, only about 30% of the total Fe III binding sites are occupied by this metal ion. As the hTF concentration in blood serum is ca.…”

“…It is interesting to note that quite similar CD spectra, giving equivalent information, were obtained with solutions containing (i) V IV OSO 4 , maltol and apo-Tf and (ii) V IV OSO 4 , dhp and apo-hTF (section ESI-6 †), thus also confirming the formation of ternary V IV O-maltol-hTF and V IV O-dhp-hTF complexes. 57 Fig. 9 presents CD spectra (recorded with a 50 mm optical path quartz cell, see the section ESI-4 †) in the 400-1000 nm range (designated here as the Vis range) of solutions containing apo-hTF and either (A) one equiv.…”

“…Strong evidence has been provided to support that most of the vanadium in the serum is bound to hTF, even in the absence of HCO 3 − . 22,23,40,43,[48][49][50][51][52][53][54][55][56][57] The results indicate that two vanadium ions are bound to the metal-ion-free apo-hTF, at the usual specific Fe III binding sites. Under the physiological conditions, particularly in the hypothetical situation where the vanadium concentration is higher than ca.…”

Thirty years through vanadium chemistry

“…Besides the possibility of vanadium or the vanadium compound entering the red blood cells and bind inside them [186,224], in the blood serum speciation can encompass ligand exchange and/or redoxinterconversion between V V and V IV (and, potentially, V III ) [225,226]. For ligand exchange, the low molecular mass constituents lactate and citrate and the high molecular mass constituents transferrin (hTF), albumin and immunoglobulin G, are the more important ones, with hTF having a predominant role [173,174,179,183,227].…”