Interaction of Trypanosoma rangeli Tejera, 1920 with different cell lines in vitro

Eger-Mangrich, Iriane; Oliveira, Marco Antônio de; Grisard, Edmundo C.; Souza, Wanderley de; Steindel, Mário

doi:10.1007/s004360000356

Cited by 27 publications

(19 citation statements)

References 15 publications

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“…The infection produced by T. rangeli is poorly understood and nothing definitive is known about the mode and the site of reproduction. Dividing blood trypomastigotes and intracellular tissue forms, such as amastigotes typical of Schizotrypanum species, have not been confirmed in vertebrate hosts of T. rangeli (Añ ez, 1981(Añ ez, , 1982D'Alessandro & Saraiva, 1992Eger-Mangrich et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Phylogeny, taxonomy and grouping of Trypanosoma rangeli isolates from man, triatomines and sylvatic mammals from widespread geographical origin based on SSU and ITS ribosomal sequences

et al. 2004

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S U M M A R YPhylogenetic relationships among Trypanosoma rangeli isolates from man, wild mammals and triatomine bugs from widespread geographical origin were inferred by comparison of the small subunit of ribosomal gene sequences. The phylogenetic trees indicated that the subgenus Herpetosoma is polyphyletic and strongly supported division of this group into two monophyletic lineages, one made up of T. rangeli, T. rangeli-like and allied species and other consisting of T. lewisi and related taxa. Based on phylogenetic analysis, morphology, behaviour in vertebrate and invertebrate hosts and epidemiology we propose : a) the validation of Herpetosoma as a taxon comprised only for species of group lewisi and the maintenance of T. lewisi as the type-species of this subgenus ; b) the classification of T. rangeli, T. rangeli-like and allied species into a ' T. rangeli-clade ' more closely related to Schizotrypanum than to T. lewisi or T. brucei. The phylogenetic tree disclosed at least 4 groups within the clade T. rangeli, all confirmed by polymorphism of the internal transcribed spacer, thus conferring for the first time phylogenetic support to groups of T. rangeli and corroborating the high complexity of this taxon. Grouping was independent of their mammalian host-species and geographical origin, indicating that other factors are determining this segregation.

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Section: Introductionmentioning

confidence: 99%

Phylogeny, taxonomy and grouping of Trypanosoma rangeli isolates from man, triatomines and sylvatic mammals from widespread geographical origin based on SSU and ITS ribosomal sequences

et al. 2004

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“…The variable T. rangeli detection levels are probably due to variable copy numbers of the satellite repeats within the T. rangeli population, as described for T. cruzi [26]. However, a parasitaemia of 10,000 T. rangeli parasites per ml of blood in human is unlikely since several studies reported no or very low T. rangeli multiplication in mammalian host cells [29,30]. The specificity of the T. cruzi OligoC-TesT was estimated at 100% on blood samples from 60 Chagas non-endemic and 48 endemic control persons.…”

Section: Discussionmentioning

confidence: 94%

T. cruzi OligoC-TesT: A Simplified and Standardized Polymerase Chain Reaction Format for Diagnosis of Chagas Disease

et al. 2009

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BackgroundPCR has evolved into one of the most promising tools for T. cruzi detection in the diagnosis and control of Chagas disease. However, general use of the technique is hampered by its complexity and the lack of standardization.MethodologyWe here present the development and phase I evaluation of the T. cruzi OligoC-TesT, a simple and standardized dipstick format for detection of PCR amplified T. cruzi DNA. The specificity and sensitivity of the assay were evaluated on blood samples from 60 Chagas non-endemic and 48 endemic control persons and on biological samples from 33 patients, 7 reservoir animals, and 14 vectors collected in Chile.Principal FindingsThe lower detection limits of the T. cruzi OligoC-TesT were 1 pg and 1 to 10 fg of DNA from T. cruzi lineage I and II, respectively. The test showed a specificity of 100% (95% confidence interval [CI]: 96.6%–100%) on the control samples and a sensitivity of 93.9% (95% CI: 80.4%–98.3%), 100% (95% CI: 64.6%–100%), and 100% (95% CI: 78.5%–100%) on the human, rodent, and vector samples, respectively.ConclusionsThe T. cruzi OligoC-TesT showed high sensitivity and specificity on a diverse panel of biological samples. The new tool is an important step towards simplified and standardized molecular diagnosis of Chagas disease.

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“…In addition, in vitro interaction of T. rangeli with dierent cell lines, using several parasite strains, showed the absence of intracellular multiplication (Osorio et al 1995;Tanoura et al 1999;Eger-Mangrich et al 2001). In addition, in vitro interaction of T. rangeli with dierent cell lines, using several parasite strains, showed the absence of intracellular multiplication (Osorio et al 1995;Tanoura et al 1999;Eger-Mangrich et al 2001).…”

Section: Discussionmentioning

confidence: 99%

Differentiation of Trypanosoma rangeli: high production of infective trypomastigote forms in vitro

et al. 2002

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In the present study, we report a simple method to induce high Trypanosoma rangeli dierentiation in vitro, producing a large number of infective trypomastigote forms. Parasites from SC-58 (Brazil) and Choachi (Colombia) strains were cultivated at 27°C in TC-100, Grace and DMEM media, each supplemented with 5% fetal bovine serum and prepared at three distinct pHs (6.0, 7.0, 8.0). Dierentiation was microscopically evaluated at 0, 3 and 6 days after cultivation in each medium by determining the percentage of trypomastigotes in Giemsa-stained smears. Our data revealed similar results for both T. rangeli strains, showing (after 6 days of cultivation in DMEM medium, pH 8.0) the presence of about 80% of trypomastigotes. These culture-derived trypomastigotes proved to be infective to both Balb-C mice and Rhodnius spp, reaching the triatomine's salivary glands. Our results describe a new and easy method to induce high T. rangeli dierentiation in vitro, allowing further studies on the antigenic constitution of trypomastigotes.

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Interaction of Trypanosoma rangeli Tejera, 1920 with different cell lines in vitro

Cited by 27 publications

References 15 publications

Phylogeny, taxonomy and grouping of Trypanosoma rangeli isolates from man, triatomines and sylvatic mammals from widespread geographical origin based on SSU and ITS ribosomal sequences

Phylogeny, taxonomy and grouping of Trypanosoma rangeli isolates from man, triatomines and sylvatic mammals from widespread geographical origin based on SSU and ITS ribosomal sequences

T. cruzi OligoC-TesT: A Simplified and Standardized Polymerase Chain Reaction Format for Diagnosis of Chagas Disease

Differentiation of Trypanosoma rangeli: high production of infective trypomastigote forms in vitro

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