Interaction of Tomato Spotted Wilt Tospovirus (TSWV) Glycoproteins with a Thrips Midgut Protein, a Potential Cellular Receptor for TSWV

Bandla, M.; Campbell, Leslie R.; Ullman, Diane E.; Sherwood, J. L.

doi:10.1094/phyto.1998.88.2.98

Cited by 111 publications

(71 citation statements)

References 33 publications

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“…Replication in mammalian or plant hosts as well as their insect vectors is a characteristic of viruses in four genera in this family (40). Previous investigations have suggested a role for the M RNA and possibly the L RNA as the location of determinants of thrips transmissibility for TSWV (7)(8)(9)32). This association was based on the accumulation of truncated L RNAs and the absence of virion formation in nontransmissible isolates (32).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have implicated G N ͞G C , which is coded on the M RNA segment, in viral attachment to the surface of epithelial cells in the thrips midgut (7)(8)(9). Point mutations or small deletions in the G N ͞G C ORF have been suggested as the cause of nontransmissibility and loss of envelope formation, although no specific mutations have been identified (32,43).…”

Section: Discussionmentioning

confidence: 99%

“…It is only known that determinants of midgut infection and transmission of La Crosse virus (Orthobunyavirus: Bunyaviridae) in the mosquito vector cosegregate with the medium (M) RNA and that proteolytic cleavage of G C is required to infect the midgut epithelium of the mosquito (3)(4)(5)(6). In the case of Tomato spotted wilt virus (TSWV) (Tospovirus: Bunyaviridae), putative thrips receptors have been identified, but there is no specific information regarding viral determinants of the virus-thrips interaction (7)(8)(9). It is as yet unclear whether there are indeed separate strategies of pathogenesis in the arthropod and mammalian or plant hosts requiring separate viral genes, or multifunctional viral genes, or if there are in fact similar pathways for pathogenesis in these divergent hosts.…”

mentioning

confidence: 99%

“…Most studies of the TSWV-thrips interactions have focused on elucidating cellular and biochemical determinants for acquisition and movement of TSWV in thrips (7)(8)(9). Several reports suggest that a receptor-mediated endocytosis governs TSWV entry to thrips midgut cells and that the TSWV G C protein and a 50-kDa thrips protein may coordinate the TSWV entry process (8,9).…”

mentioning

confidence: 99%

“…Several reports suggest that a receptor-mediated endocytosis governs TSWV entry to thrips midgut cells and that the TSWV G C protein and a 50-kDa thrips protein may coordinate the TSWV entry process (8,9). However, the G N protein was also identified as a viral attachment protein that binds to a 94-kDa thrips protein (7).…”

mentioning

confidence: 99%

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Viral genetic determinants for thrips transmission of Tomato spotted wilt virus

Sin

McNulty²,

Kennedy³

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

121

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Tomato spotted wilt virus (TSWV) is transmitted exclusively by thrips in nature. A reassortment-based viral genetic system was used to map transmissibility by thrips to the medium (M) RNA of TSWV. To locate determinants of thrips transmission in the M RNA, 30 single-lesion isolates (SLIs) were generated from a single TSWV isolate that was inefficiently transmitted by thrips. Three of the 30 SLIs were transmitted by thrips, and 27 were not. Sequence analysis of the M RNA, thrips transmissibility assays, GC protein analysis, and transmission electron microscopic studies revealed that a specific nonsynonymous mutation (C1375A) in the GN͞GC ORF of the M RNA resulted in the loss of thrips transmissibility without inhibition of virion assembly. This was in contrast to other nontransmissible SLIs, which had frameshift and͞or nonsense mutations in the GN͞GC ORF but were defective in virion assembly. The G C glycoprotein was detectable in the C1375A mutants but not in the frameshift or nonsense mutants. We report a specific viral determinant associated with virus transmission by thrips. In addition, the loss of transmissibility was associated with the accumulation of defective haplotypes in the population, which are not transmissible by thrips, rather than with the presence of a dominant haplotype that is inefficiently transmitted by thrips. These results also indicate that the glycoproteins may not be required for TSWV infection of plant hosts but are required for transmissibility by thrips.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Viral genetic determinants for thrips transmission of Tomato spotted wilt virus

Sin

McNulty²,

Kennedy³

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

121

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Inheritance genetics of the trait vector competence in Frankliniella occidentalis (Western flower thrips) in the transmission of Tomato spotted wilt virus

Ogada

Debener

Poehling

2016

Ecology and Evolution

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The complexity of tospovirus–vector–host plant interaction is linked to a range of factors influencing vector's efficacy in virus transmission, leading to high variability in the transmission efficiency within vector populations. Main shortcomings of most studies are the missing information on the intrinsic potential of individual insects to serve as efficient vectors, both at phenotypic and at genotypic levels. Moreover, detailed analysis of vector competence heredity and monitoring the splitting of both genotypes and phenotypes in filial generations has not been reported. In this study, using the model system Frankliniella occidentalis and Tomato spotted wilt virus, we evaluated the inheritance and stability of the trait vector competence in a population through basic crossings of individually characterized partners, as well as virgin reproduction. We hypothesized that the trait is heritable in F. occidentalis and is controlled by a recessive allele. From the results, 83% and 94% of competent and noncompetent males respectively, inherited their status from their mothers. The trait was only expressed when females were homozygous for the corresponding allele. Furthermore, the allele frequencies were different between males and females, and the competent allele had the highest frequency in the population. These suggest that the trait vector competence is inherited in single recessive gene in F. occidentalis, for which the phenotype is determined by the haplodiploid mechanism. These findings are fundamental for our understanding of the temporal and spatial variability within vector populations with respect to the trait vector competence and at the same time offer an essential basis for further molecular studies.

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Digestive, salivary, and reproductive organs of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) B type

Ghanim

Rosell

Campbell

et al. 2001

Journal of Morphology

106

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A microscopic analysis of the morphology and ultrastructure of the digestive, salivary, and reproductive systems of adult Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) B type was conducted using light, scanning, and transmission electron microscopy. The internal anatomy of B. tabaci was found to be similar to that reported for Trialeurodes vaporariorum. In a microscopic analysis of the salivary glands, we have shown that each primary salivary gland is composed of at least 13 cells varying in morphology and staining differentially, while the accessory salivary glands are composed of four morphologically similar cells. We analyzed the course of the alimentary canal in B. tabaci, demonstrated the internal morphology of the organs, and clarified the location of the filter chamber relative to other organs in the whitefly. Our observations confirm that the pair of structures extending from the connecting chamber are caeca that may aid in fluid movement through the midgut and are not Malpighian tubules, as previously suggested. We confirm an earlier finding that the whitefly lacks Malpighian tubules, having instead specialized Malpighian-like cells within the filter chamber at the juncture with the internal ileum. Finally, we provide the first scanning electron microscopic analysis showing the reproductive organs of B. tabaci. Our investigation provides clarified terminology for several components of the digestive and excretory system. We also provide drawings and micrographs that will aid future researchers in localizing the internal organs of B. tabaci. We expect our analysis to provide a valuable tool for studying B. tabaci / plant virus interactions and physiological and biological aspects of this insect.

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Interaction of Tomato Spotted Wilt Tospovirus (TSWV) Glycoproteins with a Thrips Midgut Protein, a Potential Cellular Receptor for TSWV

Cited by 111 publications

References 33 publications

Viral genetic determinants for thrips transmission of Tomato spotted wilt virus

Viral genetic determinants for thrips transmission of Tomato spotted wilt virus

Inheritance genetics of the trait vector competence in Frankliniella occidentalis (Western flower thrips) in the transmission of Tomato spotted wilt virus

Digestive, salivary, and reproductive organs of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) B type

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