Interaction of Stressors and the Limits of Cellular Homeostasis

Gutzeit, Herwig O.

doi:10.1006/bbrc.2001.4839

Cited by 19 publications

(12 citation statements)

References 16 publications

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“…It has been suggested that sensitization might be a prerequisite for biological effects of electromagnetic fields to be observed, or that a co-stress enhances the power-frequency magnetic-field effect (22,23). It was for this reason that whole blood rather than isolated leukocytes was used in this study, since the separation of leukocytes from blood by differential centrifugation might elicit physical stress on the isolated cells.…”

Section: Resultsmentioning

confidence: 99%

Effect of 50 Hz Electromagnetic Fields on the Induction of Heat-Shock Protein Gene Expression in Human Leukocytes

et al. 2004

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Although evidence is controversial, exposure to environmental power-frequency magnetic fields is of public concern. Cells respond to some abnormal physiological conditions by producing cytoprotective heat-shock (or stress) proteins. In this study, we determined whether exposure to power-frequency magnetic fields in the range 0-100 T rms either alone or concomitant with mild heating induced heat-shock protein gene expression in human leukocytes, and we compared this response to that induced by heat alone. Samples of human peripheral blood were simultaneously exposed to a range of magnetic-field amplitudes using a regimen that was designed to allow field effects to be distinguished from possible artifacts due to the position of the samples in the exposure system. Power-frequency magnetic-field exposure for 4 h at 37؇C had no detectable effect on expression of the genes encoding HSP27, HSP70A or HSP70B, as determined using reverse transcriptase-PCR, whereas 2 h at 42؇C elicited 10-, 5-and 12-fold increases, respectively, in the expression of these genes. Gene expression in cells exposed to power-frequency magnetic fields at 40؇C was not increased compared to cells incubated at 40؇C without field exposure. These findings and the extant literature suggest that power-frequency electromagnetic fields are not a universal stressor, in contrast to physical agents such as heat. ᭧

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Section: Resultsmentioning

confidence: 99%

Effect of 50 Hz Electromagnetic Fields on the Induction of Heat-Shock Protein Gene Expression in Human Leukocytes

et al. 2004

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“…ELF‐EMF has been shown to induce significant biological alterations in a variety of cells and tissues (Hong 1995; Goodman & Blank 1998; Gutzeit 2001). These changes include the induction of several early response genes, including c‐ myc (Jin et al .…”

Section: Discussionmentioning

confidence: 99%

“…The interaction of stressors and the physiological consequences on the cellular and molecular level have not been explored, although the importance of stress‐induced reactions for a number of medical questions is apparent (Gutzeit 2001). A substitution of current hyperthermia treatment by ELF‐EMF exposure appears to be a strategy worth pursuing.…”

Section: Discussionmentioning

confidence: 99%

“…ELF-EMF has been shown to induce significant biological alterations in a variety of cells and tissues (Hong 1995;Gutzeit 2001). These changes include the induction of several early response genes, including c-myc (Jin et al 2000), c-fos (Rao & Henderson 1996) and HSP70 mRNA and resulted in an elevated level of stress inducible HSP70 protein production (Goodman & Henderson 1988).…”

Section: Discussionmentioning

confidence: 99%

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The heat shock‐induced cell cycle arrest is attenuated by weak electromagnetic fields

Tokalov

Gutzeit

2003

Cell Proliferation

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Stress-induced effects in human acute leukaemia cells (HL-60) were studied by flow cytometry using the fluorescent dye carboxyfluorescein succinimidyl ester which allows the analysis of several successive cell generations for up to 10 days. Asynchronously cycling cells subjected to heat shock (30 min at 41 degrees C) responded in two distinct ways: while one fraction of the cell population (about 15%) re-entered the cell cycle after a short delay, other cells became arrested at different phases of the cell cycle and remained arrested for up to several days and finally underwent apoptosis. Weak electromagnetic fields (60 micro T, 50 Hz) alleviated the heat-induced block and the fraction of arrested cells was significantly smaller.

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“…Interactions between some magnetic field exposure conditions and other stressors, for example heat, have also been suggested as a necessary condition for magnetic fields to influence the HSP response [Gutzeit, 2001]. This would suggest that magnetic fields act as a weak stressor, which on their own cannot necessarily induce a heat shock response.…”

Section: Mechanisms: Heat Shock Proteinsmentioning

confidence: 99%

The influence of extremely low frequency magnetic fields on cytoprotection and repair

Robertson

Thomas

Bureau

et al. 2006

Bioelectromagnetics

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Ischemia-reperfusion injuries, such as those suffered from various types of cardiovascular disease, are major causes of death and disability. For relatively short periods of ischemia, much of the damage is potentially reversible and in fact, does not occur until the influx of oxygen during the reperfusion stage. Because of this, there is a window of opportunity to protect the ischemic tissue. Here, we review several mechanisms of protection, such as heat shock proteins, opioids, collateral blood flow, and nitric oxide induction, and the evidence indicating that magnetic fields may be used as a means of providing protection via each of these mechanisms. While there are few studies demonstrating direct protection with magnetic field therapies, there are a number of published reports indicating that electromagnetic fields may be able to influence some of the biochemical systems with protective applications.

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Interaction of Stressors and the Limits of Cellular Homeostasis

Cited by 19 publications

References 16 publications

Effect of 50 Hz Electromagnetic Fields on the Induction of Heat-Shock Protein Gene Expression in Human Leukocytes

Effect of 50 Hz Electromagnetic Fields on the Induction of Heat-Shock Protein Gene Expression in Human Leukocytes

The heat shock‐induced cell cycle arrest is attenuated by weak electromagnetic fields

The influence of extremely low frequency magnetic fields on cytoprotection and repair

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