Interaction of Sorghum Tannins with Wheat Proteins and Effect on in Vitro Starch and Protein Digestibility in a Baked Product Matrix

Dunn, Kristen L.; Yang, Liyi; Girard, Audrey L.; Bean, Scott R.; Awika, Joseph M.

doi:10.1021/jf504112z

Cited by 57 publications

(20 citation statements)

References 41 publications

(92 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Phenolic content was measured according to the Folin–Ciocalteu method. 37 Total protein concentration was determined by combustion using LECO instrument and a nitrogen-to-protein conversion factor of 5.7. 37 Total starch content was found using the megazyme assay kit, based on the RTS-NaOH procedure from the AACC 76.13 and AOAC 999.11 methods (K-TSTA; Megazyme, Bray, Ireland).…”

Section: Methodsmentioning

confidence: 99%

Rapid and Noninvasive Typing and Assessment of Nutrient Content of Maize Kernels Using a Handheld Raman Spectrometer

2019

View full text Add to dashboard Cite

To thrive as a global civilization, food production must meet the demands of our ever-growing population. There are more than a billion people on the planet suffering from malnutrition through poor quality or lack of food. Nutrient content of food can be determined by a variety of methods, which have issues such as slow analysis or sample destruction. Near-infrared (NIR) spectroscopy is a long-standing alternative to these methods. In this work, we demonstrated that Raman spectroscopy (RS), another spectroscopic method, can also be used to assess the nutrient content of maize (Zea mays), one of the most widely cultivated grains in the world. Using a handheld Raman spectrometer, we predicted the content of carbohydrates, fibers, carotenoids, and proteins in six different varieties of maize. This analysis requires only a single maize kernel and is fast (1s), portable, noninvasive, and nondestructive. Moreover, we showed that RS in combination with chemometric methods can be used for highly accurate (approximately 90%) spectroscopic typing of maize, which is important for plant breeders and farmers. Finally, we demonstrate that Raman-based approach is as accurate as NIR analysis. These findings suggest that portable Raman systems can be used on combines and grain elevators for autonomous control of grain quality.

show abstract

Section: Methodsmentioning

confidence: 99%

Rapid and Noninvasive Typing and Assessment of Nutrient Content of Maize Kernels Using a Handheld Raman Spectrometer

2019

View full text Add to dashboard Cite

show abstract

“…The degradation of pea protein and tannic acid (TA) complexes was studied in the simulated gastro-intestinal model [ 19 ]. The digestion of complexes based on sorghum TA and wheat proteins was studied in the presence of the α-amylase and amyloglucosidase [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Degradation of Hybrid Drug Delivery Carriers with a Mineral Core and a Protein–Tannin Shell under Proteolytic Hydrolases

et al. 2022

View full text Add to dashboard Cite

Hybrid carriers with the mineral CaCO3/Fe3O4 core and the protein–tannin shell are attractive for drug delivery applications due to reliable coupling of anticancer drugs with protein–tannin complex and the possibility of remote control over drug localization and delivery by the external magnetic field. This study aims to elucidate the mechanisms of drug release via enzymatic degradation of a protein–tannin carrier shell triggered by proteolytic hydrolases trypsin and pepsin under physiological conditions. To do this, the carriers were incubated with the enzyme solutions in special buffers to maintain the enzyme activity. The time-lapse spectrophotometric and electron microscopy measurements were carried out to evaluate the degradation of the carriers. It was established that the protein–tannin complex demonstrates the different degradation behavior depending on the enzyme type and buffer medium. The incubation in trypsin solution mostly resulted in the protein shell degradation. The incubation in pepsin solution did not affect the protein component; however, the citric buffer stimulates the degradation of the mineral core. The presented results allow for predicting the degradation pathways of the carriers including the release profile of the loaded cargo under physiological conditions. The viability of 4T1 breast cancer cells with mineral magnetic carriers with protein–tannin shells was investigated, and their movement in the fields of action of the permanent magnet was shown.

show abstract

“…However, given that starch is only partially gelatinized in most starchy foods (e.g., cookies -2-11%, crackers -3%, bread -33-71%, cereal flakes -24-27% (Varriano-Marston, Ke, & Huang, 1980;Wootton & Chaudhry, 1980), and granule integrity is largely retained, it is not clear how above observations would translate into a typical food system. Dunn, Yang, Girard, Bean, and Awika (2015) reported that tortilla (in which starch is partially gelatinized) processed from wheat flour with added high tannin sorghum bran (rich in high MW PA), increased SDS from 13 to 21% (compared to control and non-tannin brans) without an increase in RS formation. Extensive interaction of PA with gluten proteins during the dough mixing stage was observed; thus it was not clear whether the protein-PA interactions influenced the observed changes in starch digestibility.…”

Section: Introductionmentioning

confidence: 99%

Polymeric tannins significantly alter properties and in vitro digestibility of partially gelatinized intact starch granule

Amoako

Awika

2016

Food Chemistry

Self Cite

View full text Add to dashboard Cite

Excess calorie intake is a growing global problem. This study investigated effect of complexing partially gelatinized starch with condensed tannins on in vitro starch digestibility. Extracts from tannin and non-tannin sorghum, and cellulose control, were reacted with normal and waxy maize starch in 30% (30E) and 50% ethanol (50E) solutions at 70°C/20min. More tannins complexed with the 30E than 50E starches (mean 6.2 vs 3.5mg/g, respectively). In the 30E treatments, tannins significantly increased crystallinity, pasting temperature, peak viscosity, and slow digesting starch (from 100 to 274mg/g) in normal, but not waxy starch, suggesting intragranular cross-linking with amylose. Tannins doubled resistant starch (RS) to approx. 300mg/g in both starches. In 50E treatments, tannins made both maize starches behave like raw potato starch (>90% RS), suggesting granule surface interactions dominated. Non-tannin treatments generally behaved similar to cellulose. Condensed tannins could be used to favorably alter starch digestion profile.

show abstract

Interaction of Sorghum Tannins with Wheat Proteins and Effect on in Vitro Starch and Protein Digestibility in a Baked Product Matrix

Cited by 57 publications

References 41 publications

Rapid and Noninvasive Typing and Assessment of Nutrient Content of Maize Kernels Using a Handheld Raman Spectrometer

Rapid and Noninvasive Typing and Assessment of Nutrient Content of Maize Kernels Using a Handheld Raman Spectrometer

Degradation of Hybrid Drug Delivery Carriers with a Mineral Core and a Protein–Tannin Shell under Proteolytic Hydrolases

Polymeric tannins significantly alter properties and in vitro digestibility of partially gelatinized intact starch granule

Contact Info

Product

Resources

About