Interaction of sn-glycerol 3-phosphorothioate with Escherichia coli: effect on cell growth and metabolism

Abstract

sn-Glycerol 3-phosphorothioate was found to be bacteriocidal to strains of Escherichia coli which have a functional sn-glycerol 3-phosphate transport system. This effect was manifest in strains 7 and 8, which are constitutive mutants for the utilization and transport of sn-glycerol 3-phosphate (glpRc2). Strain E15, which is considered to be wild type for the glycerol phosphate functional units, was affected by the phosphorothioate analog only under conditions that are known to induce the transport system for sn-glycerol 3-phosphate. In addition, another strain of E. coli, strain 6, which is isogenic with strain E15 but has an impaired sn-glycerol 3-phosphate transport system (glpT13), was not affected by similar concentrations of sn-glycerol 3-phosphorothioate. Transport studies in which [3H]glycerol phosphate and its phosphorothioate analog were used demon-789

Production of ?-(−)-3-hydroxyalkanoic acid by recombinant Escherichia coli

“…It was reported that inhibition of phospholipid synthesis led to acyl-ACP accumulated in E. coli [21]. Thus, inhibitors of phospholipids synthesis such as dihydroxylbutyryl-1-phosphonate [22] and sn-glycerol-3-phosphoro-thioate [23] may also serve as a useful tool for improvement on mcl-mcl-3HA production from expression of phaG Pp .…”