Interaction of rice salinity screening in germination and seedling phase through selection index based on principal components

Abstract

Salinity stress can reduce rice (Oryza sativa L.) productivity and cause crop failure. This problem needs a solution by development of tolerant varieties, and this development closely relates to the effectiveness of its screening. Screening at the phase of germination and seedling are the most common ones. However, the interactions between screenings based on the growth character selection index have not been widely reported, particularly with the principal component analysis (PCA). Therefore, the aim of this study was to determine the interaction between phases of rice salinity screening through a selection index based on PCA. This study consisted of two phases of salinity screening, namely growth phase of germination and seedlings. Both screenings were designed with a nested randomized complete block design, where replicates were nested in a selection environment. The selection environment consisted of two levels, normal (0 mM NaCl) and saline (120 mM NaCl). The genotypes consisted of eight varieties and was repeated three times. Observations were based on morphological and physiological characters, especially in seedling phase screening. Results showed that morphological character approach of the seedlings had a large distribution of salinity tolerances. The use of stress tolerance index and PCA were considered effective in the formation of the selection index on salinity screening. As for, the morphology index was formulated as 0.32 shoot height + 0.33 root fresh weight + 0.33 shoot fresh weight + 0.26 root length + 0.01 number of tillers, length + 0.34 total biomass fresh weight. Therefore, the use of this analytical concept is recommended in screening the tolerance of rice lines to salinity stress.

Respon Galur-galur Harapan Padi Sawah Dihaploid terhadap Salinitas pada Fase Bibit

“…Berdasarkan beberapa analisis di atas, karakter yang berhubungan dengan tajuk lebih dapat mencirikan toleransi salinitas pada fase bibit dibandingkan karakter terkait akar. Hal ini sejalan dengan berbagai hasil penelitian (Ali et al, 2014;Nayyeripasand et al, 2019;Anshori et al, 2021;Yadav et al, 2021). Selain itu, hasil analisis menunjukkan bahwa penurunan tinggi bibit dapat direkomendasikan sebagai karakter seleksi dalam penapisan genotipe padi terhadap salinitas pada fase bibit.…”

“…Meskipun tidak seketat penurunan tinggi bibit dalam menapis salinitas, penurunan pada bobot basah tajuk, bobot kering tajuk, dan bobot kering biomassa juga dapat dipertimbangkan sebagai karakter seleksi berdasarkan analisis di atas. Penggunaan karakter penurunan bobot basah tajuk (Anshori et al, 2021) atau penurunan pada bobot basah tajuk, bobot kering tajuk, dan bobot kering biomassa (Anshori, 2018) dalam proses seleksi genotipe padi toleran salinitas pernah dilaporkan sebelumnya.…”

“…Evaluasi perubahan morfologi pada fase vegetatif dilakukan dengan memberikan perlakuan salinitas terhadap bibit tanaman padi, lalu dilakukan skoring terhadap kerusakan visual bibit berdasarkan Standard Evaluation System (SES) for rice dari IRRI (Egdane et al, 2003). Pengamatan tambahan dapat dilakukan terhadap tinggi bibit, panjang akar, bobot basah tajuk, dan lain-lain (Safitri et al, 2017;Kargbo et al, 2019;Farid et al, 2021). Galur-galur padi doubled-haploid (DH) hasil kultur antera berhasil diperoleh (Safitri et al, 2016) dan telah dilakukan serangkaian pengujian daya hasil pada beberapa lokasi (Hidayatullah et al, 2018).…”

Synthesis and characterization of silica nanoparticles from rice husk and their effects on physiology of rice under salt stress

“…Salinity is one of the main abiotic stresses which threatens rice production at varying stages from seed germination, seedling, vegetative through to reproductive growth (Farid et al, 2021). In Thailand, rice is most widely cultivated in the northeast of the country where soils are affected by salinity, causing low yields compared to other geographical locations (Kanawapee et al, 2012).…”

Evaluation to Select Tomato Genotypes with Big Fruit and Verification of Genetic Advance

“…Index selection is one of the selection methods for multiple characters commonly used in addition to tandem selection and independent culling [9,10]. Hazel and Lush [11] and Lin [12] investigated the relative efficacy of tandem selection, independent culling levels, and index selection.…”