Interaction of protonated DNA with trans-dichlorodiammineplatinum(II)

Kasyanenko, N. A.; Prokhorova, S. A.; Enriquez, E.F. Haya; Sudakova, S.S; Frisman, E.V.; D'yachenko, S. A.; Smorygo, N. A.; Ivin, B. A.

doi:10.1016/s0927-7757(98)00599-8

Cited by 13 publications

(7 citation statements)

References 17 publications

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“…The binding of positively charged ligands to N7G causes specific changes in the DNA absorption: slight hypochromism that at high concentrations turns into hyperchromism at 260 nm and an emergence of a shoulder at λ > 270 nm. − The experimental data indicate competition for the binding sites on DNA between CoPc and Mn 2+ (Figure a,b).…”

Section: Resultsmentioning

confidence: 96%

DNA Complexes with Cobalt(II) Phthalocyanine Disodium Disulfonate

et al. 2019

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The interaction of cobalt phthalocyanine disodium disulfonate (CoPc) with calf thymus DNA in solutions was investigated by UV/vis spectrophotometry, circular dichroism (CD), and hydrodynamic methods (viscosity and flow birefringence). Two types of CoPc binding to DNA were observed. Fast CoPc interactions with DNA via external binding to phosphates were accompanied by the formation of stack-type phthalocyanine structures on the periphery of the DNA helix. The optical absorption spectra of such CoPc complexes with DNA were analyzed in order to obtain a binding constant K = (4.8 ± 0.4) × 104 M–1. CD spectra show the increasing optical activity of phthalocyanines bonded to DNA. DNA plays the role of a matrix, contributing to an increase in their stacking interactions. The CD spectrum of DNA varies slightly. The second type of cobalt-to-DNA binding manifests itself over a certain time. It can be associated with the reorganization of ligands in the cobalt coordination sphere by introducing DNA atoms. In our experiments, such binding was observed after storage of solutions for approximately 20 h at a temperature of 4 °C. It was shown that the minor groove of DNA remains free in CoPc–DNA complexes. CoPc does not bind with the most important group for metal coordinating to DNA in the major groove (N7 guanine). We completely excluded the intercalation binding model. The planes of phthalocyanines in CoPc–DNA complexes are oriented predominantly normal to the axis of the DNA helix. DNA rigidity (persistent length) does not change. This follows from the data on the measurement of the optical anisotropy and intrinsic viscosity of DNA in complexes.

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Section: Resultsmentioning

confidence: 96%

DNA Complexes with Cobalt(II) Phthalocyanine Disodium Disulfonate

et al. 2019

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“…Spectral data show an increase in DNA absorption with a decrease in the intensity of the CD positive band. Local bending of DNA helix can also induce a decrease in the optical anisotropy of DNA [ 21 , 48 ]. Trans-DDP binding also induces the decrease in the volume of the DNA molecular coil, but the increase in (α 1 − α 2 ) value is observed.…”

Section: Resultsmentioning

confidence: 99%

“…Another way to test the formation of the platinum coordination bonds with N7 of guanine is to study the protonation of DNA molecules in complexes with platinum compounds [ 48 ]. The absorption and the CD spectra of the protonated DNA [ 48 , 69 , 70 ] reflect the emergence of positive charges on the DNA bases. It is known that the primary proton acceptor group for double-stranded DNA is N7G.…”

Section: Resultsmentioning

confidence: 99%

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DNA Conformational Changes Induced by Its Interaction with Binuclear Platinum Complexes in Solution Indicate the Molecular Mechanism of Platinum Binding

et al. 2022

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Platinum anticancer drugs inhibit the division of cancer cells through a DNA binding mechanism. The bimetallic platinum compounds have a possibility for blocking DNA replication via the cross-linking of DNA functional groups at different distances. Many compounds with metals of the platinum group have been tested for possible antitumor activity. The main target of their biological action is a DNA molecule. A combined approach to the study of the interaction of DNA with biologically active compounds of this type is proposed. The capabilities of various methods (hydrodynamic, spectral, microscopy) in obtaining information on the type of binding of coordination compounds to DNA are compared. The analysis of DNA binding with platinum binuclear compounds containing pyrazine, tetrazole, 5- methyltetrazole, 3-propanediamine as bridging ligands in a solution was carried out with the methods of circular dichroism (CD), luminescent spectroscopy (LS), low gradient viscometry (LGV), flow birefringence (FB) and atomic force microscopy (AFM). The competitive binding of different platinum compounds to DNA and the analysis of platinum attachment to DNA after protonation of its nitrogen bases simply indicates the involvement of N7 guanine in binding. Fluorescent dye DAPI was also used to recognize the location of platinum compounds in DNA grooves. DNA conformational changes recorded by variations in persistent length, polyelectrolyte swelling, DNA secondary structure, and its stability clarify the molecular mechanism of the biological activity of platinum compounds.

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“…It is well known that the decrease in pH causes the appearance of positive charges on nitrogen bases together with the negative charges on phosphates of DNA. It can be fixed as change in UV-and CD DNA spectra [41][42][43]. Experiments have demonstrated that low polymer concentration (N/P < 1) has no influence on DNA protonation (Fig.…”

Section: Resultsmentioning

confidence: 99%

DNA interaction with synthetic polymers in solution

et al. 2007

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DNA complexes with cationic polymers (polyvinylamine (PVA), polyallylamine (PAA), polydimethylaminoethylmethacrylate (PDMAEM), poly-(N,N,Ntrimethylammonio)ethyl methacrylate chloride (PTMA EM), poly-L-lysine (PLL)) were investigated. It was shown that volume and persistent length of DNA do not change essentially at low cationic polymer concentration in a solution. DNA packaging in 0.005 M NaCl was observed at charge ratio N/P % 1. Secondary DNA structure in complexes was not disrupted, and DNA was protected from protonation. The comparison between DNA packaging in complexes with polycations and DNA condensation induced by trivalent ions was made.

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Interaction of protonated DNA with trans-dichlorodiammineplatinum(II)

Cited by 13 publications

References 17 publications

DNA Complexes with Cobalt(II) Phthalocyanine Disodium Disulfonate

DNA Complexes with Cobalt(II) Phthalocyanine Disodium Disulfonate

DNA Conformational Changes Induced by Its Interaction with Binuclear Platinum Complexes in Solution Indicate the Molecular Mechanism of Platinum Binding

DNA interaction with synthetic polymers in solution

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