Interaction of polysaccharides with plasma membranes—I. Interaction of human erythrocytes with degraded iota carrageenans and the effect of dextran and DEAE dextran

Ep, Pittz; Jones, Rose M.; Goldberg, L; Coulston, F.

doi:10.3233/bir-1977-14103

Cited by 9 publications

(5 citation statements)

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“…Rouleau formation experiments are performed at this hematocrit and at lower hematocrits, typically 0.5%-5%, where individual red cells and rouleau can be distinguished (cf. Kernick et al, 1973;Pittz et al, 1977). A 1% hematocrit corresponds to 1.07 x 10o' erythrocytes/mm3 (Miale, 1977); thus No/V 107 red cells/mm3 and N/ Vis determined by the experimental hematocrit.…”

Section: Numerical Evaluations and Resultsmentioning

confidence: 97%

The equilibrium size distribution of rouleaux

Perelson¹,

Wiegel²

1982

Biophysical Journal

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Rouleaux are formed by the aggregation of red blood cells in the presence of macromolecules that bridge the membranes of adherent erythrocytes. We compute the size and degree of branching of rouleaux for macroscopic systems in thermal equilibrium in the absence of fluid flow. Using techniques from statistical mechanics, analytical expressions are derived for (a) the average number of rouleaux consisting of n cells and having m branch points; (b) the average number of cells per rouleau; (c) the average number of branch points per rouleau; and (d) the number of rouleaux with n cells, n = 1, 2, ..., in a system containing a total of N cells. We also present the results of numerical evaluations to establish the validity of asymptotic expressions that simplify our formal analytic results.

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Section: Numerical Evaluations and Resultsmentioning

confidence: 97%

The equilibrium size distribution of rouleaux

Perelson¹,

Wiegel²

1982

Biophysical Journal

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“…Several authors have shown that neutral polymers can adsorb onto cell membranes (Brooks and Seaman, 1973a,d;Pfafferott et al, 1982;Pittz et al, 1977). Brooks and Seaman (1973a,d) demonstrated that the electrokinetic potential of red blood cells change when suspended in solutions containing several polymers of various molecular weights, including dextran, Pluronic F-68, and methylcellulose.…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of Methylcellulose and Other Polymers on Insect Cells Subjected to Laminar Shear Stress

Goldblum

Bae

Hink

et al. 1990

Biotechnology Progress

127

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The relative sensitivity of two insect cell lines to laminar shear stress was determined, and the protective effect of polymers added to the growth media of two insect cell lines, Trichoplusia ni (TN-368) and Spodoptera frugiperda (SF-9), was evaluated. TN-368 and SF-9 cells were found to be equally sensitive to laminar shear stress. Methylcellulose [0.5% (w/v) Dow E4M Methocel] and dextran [4.5% (w/v)] increased the resistance of suspended cells to lysis due to laminar shear stress by factors of up to 76 and 28, respectively, compared to cells in media without additives. It was observed that the protective effect of Pluronic F-68 was concentration-dependent: 0.2% and 0.3% (w/v) F-68 increased the resistance of SF-9 cells to shear stress by factors of 15 and 42, respectively. However, increasing the concentration to 0.5% did not significantly increase the cells' resistance compared to 0.3% (w/v). F-68 at 0.2% only increased the resistance of TN-368 cells by a factor of 6. It is believed that the protection is a result of the polymer adsorbing to the cell membrane. None of the polymer additives tested had a significant effect on SF-9 or TN-368 growth rate.

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“…On the other hand, erythrocytes have been shown to bind sulfated polysaccharides like carrageenan [51]. There is evidence that surface glycosyltransferases catalyse the glycosylation of the terminal end of the matching sugar chain [52]. Carrageenan may interfere with the linking of the terminal sugars of glycoconjugates on the cell surface.…”

Section: Carrageenan Carrageenan (Figurementioning

confidence: 99%

Toxicological and Teratogenic Effect of Various Food Additives: An Updated Review

Sambu

Hemaram

Rajadurai

et al. 2022

BioMed Research International

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Scientific evidence is mounting that synthetic chemicals used as food additives may have harmful impacts on health. Food additives are chemicals that are added to food to keep it from spoiling, as well as to improve its colour and taste. Some are linked to negative health impacts, while others are healthy and can be ingested with little danger. According to several studies, health issues such as asthma, attention deficit hyperactivity disorder (ADHD), heart difficulties, cancer, obesity, and others are caused by harmful additives and preservatives. Some food additives may interfere with hormones and influences growth and development. It is one of the reasons why so many children are overweight. Children are more likely than adults to be exposed to these types of dietary intakes. Several food additives are used by women during pregnancy and breast feeding that are not fully safe. We must take specific precaution to avoid consuming dangerous compounds before they begin to wreak havoc on our health. This study is intended to understand how the preservatives induce different health problem in the body once it is consumed. This review focuses on some specific food additives such as sodium benzoate, aspartame, tartrazine, carrageenan, and potassium benzoate, as well as vitamin A. Long-term use of food treated with the above-mentioned food preservatives resulted in teratogenicity and other allergens, according to the study. Other health issues can be avoided in the future by using natural food additives derived from plants and other natural sources.

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Interaction of polysaccharides with plasma membranes—I. Interaction of human erythrocytes with degraded iota carrageenans and the effect of dextran and DEAE dextran

Cited by 9 publications

References 0 publications

The equilibrium size distribution of rouleaux

The equilibrium size distribution of rouleaux

Protective Effect of Methylcellulose and Other Polymers on Insect Cells Subjected to Laminar Shear Stress

Toxicological and Teratogenic Effect of Various Food Additives: An Updated Review

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