Interaction of Polyethyleneimines with Fibrinogen and Erythrocyte Membrane

Zhong, Dagen; Zhang, Yuanming; Zuo, Qinhua; Liu, Zonghua; Wang, Xue

doi:10.1080/1539445x.2013.812873

Cited by 7 publications

(7 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To further compare the different effects of mPEG2k–PCL2k and mPEG5k–PCL2k, a rheological experiment was conducted to record the viscoelasticity changes of whole blood containing the copolymers as a function of time . The time‐dependent storage modulus ( G ′) and loss modulus ( G ″) of the whole blood/mPEG–PCL samples are shown in Figure .…”

Section: Resultsmentioning

confidence: 99%

“…Platelet‐rich plasma (PRP) was obtained by centrifuging citrated whole blood at 100 g for 10 min, and platelet‐poor plasma (PPP) was collected by spinning the citrated whole blood at 1000 g for 10 min. The following methods refer to the previous studies …”

Section: Methodsmentioning

confidence: 99%

“…The following methods refer to the previous studies. [12][13][14][15] APTT and PT assays The mPEG-PCL solutions were mixed with the PPP at a volume ratio of 1:9. The APTT and PT of the samples were measured at 37 C on a STA-R Evolution automatic coagulation analyzer (Stago, France) with the addition of relevant reagents provided by the Stago Company.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Hemocompatibility evaluation in vitro of methoxy polyethyleneglycol–polycaprolactone copolymer solutions

Zhang

Wang

et al. 2016

J Biomedical Materials Res

Self Cite

View full text Add to dashboard Cite

Amphiphilic block copolymer methoxy polyethyleneglycol-polycaprolactone (mPEG-PCL) has attracted interest in the biomedical field, due to its water solubility and biodegradability. Nevertheless, the blood safety of mPEG-PCL copolymers has not been investigated in detail. Because mPEG-PCL copolymers introduced in vivo would inevitably interact with blood tissue, an investigation of possible interactions of mPEG-PCL with key blood components is crucial. We studied the effects of two mPEG-PCL copolymer solutions on blood coagulation, the morphology and lysis of human red blood cells (RBCs), the structure of plasma fibrinogen, complement activation, and platelet aggregation. We found that higher concentrations of the mPEG-PCL copolymers impaired blood clotting, and the copolymers had little impact on the morphology or lysis of RBCs. From the spectroscopy results, the copolymers affected the local microstructure of fibrinogen. The copolymers significantly activated the complement system in a concentration-dependent way. At higher concentrations, the copolymers impaired platelet aggregation, which may have been mediated by an inhibition of the arachidonic acid pathway. These findings provide important information that may be useful for the molecular design and biomedical applications of mPEG-PCL copolymers. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 802-812, 2016.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Hemocompatibility evaluation in vitro of methoxy polyethyleneglycol–polycaprolactone copolymer solutions

Zhang

Wang

et al. 2016

J Biomedical Materials Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…Following procedures previously reported in literature [24,32], the amount of adsorbed proteins was determined in an indirect way by measuring the non-adsorbed proteins and subtracting this value from the initial known protein feed. For the quantification of the protein in the PBS solutions, the characteristic absorbance of the Fibrinogen proteins at 280 nm [33] was analyzed on a HP 8453 UV-Vis spectrophotometer equipped with a Peltier cell using a quartz cuvette (1 cm path length). Each coating was measured in triplicate.…”

Section: Characterization Techniques and Proceduresmentioning

confidence: 99%

Hydrophilic Self-Replenishing Coatings with Long-Term Water Stability for Anti-Fouling Applications

et al. 2018

View full text Add to dashboard Cite

Hydrophilic coatings have recently emerged as a new approach to avoiding the adhesion of (bio)organisms on surfaces immersed in water. In these coatings the hydrophilic character is crucial for the anti-fouling (AF) performance. However, this property can be rapidly lost due to the inevitable damages which occur at the surface, reducing the long-term effectiveness of the AF functionality. We report hydrophilic polycarbonate-poly(ethylene glycol) methyl ether (mPEG) polyurethane coatings with tunable hydrophilic properties as well as an excellent and long-term stability in water. The coatings exhibit low protein adhesion values and are able to self-replenish their hydrophilicity after damage, due to the existence of a reservoir of hydrophilic dangling chains incorporated in the bulk. The combination of low T g and sufficient mobility of the mPEG dangling chains (enabled by chains with higher molecular weight) proved to be crucial to ensure autonomous surface hydrophilicity recovery when the coatings were immersed in water. This coatings and design approach offers new possibilities towards high-performance AF coatings with an extended service life-time which can be used in several major applications areas, such as marine and biomedical coatings, with major economic and environmental benefits.

show abstract

“…The quantification of the adsorbed FB was done by UV/Vis spectroscopy and making use of the characteristic absorption peak of these proteins at 280 nm, due to the presence of aromatic aminoacids in their chemical structure. [24][25] The coatings were fully covered with a known amount of a PBS solution of FB. The adsorbed proteins were quantified by determining the proteins remaining in the PBS solution (not-absorbed) after 24 hours of incubation and with reference to a previously prepared calibration curve (see Figure S3 in Supporting Information).…”

Section: Evaluation Of Anti-fouling Propertiesmentioning

confidence: 99%

Hydrophilic Self-Replenishing Coatings with Long-Term Water Stability for Anti-Fouling Applications

Jiménez-Pardo¹,

Ven²,

Benthem³

et al. 2018

Preprint

View full text Add to dashboard Cite

show abstract

Interaction of Polyethyleneimines with Fibrinogen and Erythrocyte Membrane

Cited by 7 publications

References 22 publications

Hemocompatibility evaluation in vitro of methoxy polyethyleneglycol–polycaprolactone copolymer solutions

Hemocompatibility evaluation in vitro of methoxy polyethyleneglycol–polycaprolactone copolymer solutions

Hydrophilic Self-Replenishing Coatings with Long-Term Water Stability for Anti-Fouling Applications

Hydrophilic Self-Replenishing Coatings with Long-Term Water Stability for Anti-Fouling Applications

Contact Info

Product

Resources

About