Interaction of pleurocidin and its analogs with phospholipid membrane and their antibacterial activity

Yoshida, Kanako; Mukai, Yasuhiro; Niidome, Takuro; Takashi, Chinami; Tokunaga, Yoshiharu; Hatakeyama, Tomomitsu; Aoyagi, Haruhiko

doi:10.1034/j.1399-3011.2001.00802.x

Cited by 66 publications

(71 citation statements)

References 35 publications

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“…Unfortunately, data are lacking; however, a microglia pump that extrudes cytoplasmic protons (the vacuolar pump accumulates protons) is potentiated by cell swelling (Morihata et al, 2000). Respiratory cytochrome oxidase Peptide fragments from modern channels 23-aa / M2 from acetylcholine channel (Montal et al, 1990) 22-aa/ voltage-sensitive sodium channel (Oiki et al, 1988) 22-aa/ S4, repeat IV of sodium channel (Tosteson et al, 1989) XX-aa / S2 or S3 from calcium channels (Grove et al, 1993) 23-aa / M2 of brain glycine receptor anion-selective channel (Reddy et al, 1993) 26-aa /"H5" from Shaker; anion channel (Shinozaki et al, 1994) Assorted channel-forming biopeptides 20-aa/ alamethicin (Futaki, 1998) 10-aa ϫ 2 / gramicidin (dimers span the membrane) (Futaki, 1998) (Yoshida et al, 2001) Assorted channel-forming synthetic peptides 24-aa, synthetic, can be non-selective or cation selective. Two mode channels induced by interaction of acidic amphiphilic ␣-helical peptides with lipid bilayers ) 24-aa, 3(10)-helical peptides (e.g.…”

Section: Leaks Pumps and Tension In A Protocellmentioning

confidence: 99%

How did cells get their size?

Morris

2002

Anat. Rec.

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Cells exercise size homeostasis, and the origins of their ability to do so is the topic of this essay. Before there were cells, there were protocells. The most basic questions about protocells as objects are: What were they made of, and how big were they? Asking how big they were implies that the answer to the first part includes a boundary. The best candidate for that boundary is a self-assembling lipid bilayer. Therefore, protocells are defined here as Darwinian liposomes-bilayer vesicles with mutable on-board replicases linked to phenotypes. Because liposomes undergo spontaneous fission and fusion, and are subject to osmotic forces, size regulation in the earliest protocells would essentially have been liposome physics. For successful protocells, averting osmotic lysis would have been the first order of business. However, from the outset size mattered too, because of sex and reproduction (i.e., genome mixing and genome copying in entities with phenotypes). Protocell fission and fusion would have blended seamlessly into protocell sex and reproduction, making any gene product that furnished control over protocell size changes doubly adaptive. A recurrent theme is the feedback role of bilayer tension in protocell size control. Ways in which primitive peptides and their aggregates (e.g., channels) might have allowed liposomes to gain improved volume and surface area homeostasis are suggested. Domain-swapped proteins that polymerize as filaments are discussed as the origin of cytoskeleton structures that diversify and stabilize liposome shapes and sizes. Throughout, attention is paid to the question of set points for cell size.

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Section: Leaks Pumps and Tension In A Protocellmentioning

confidence: 99%

How did cells get their size?

Morris

2002

Anat. Rec.

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“…Pleurocidin was isolated from the winter flounder Pleuronectes americanus (Walbaum) in 1997 (9), and its activity has been under investigation by several research groups (13,20,21,23,28). Also, pleurocidin genes have been cloned, and their genetic organization has been described (8, 10; S. E. Douglas, A. Patrzykat, J. Pytyck, and J. W. Gallant, submitted for publication).…”

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confidence: 99%

Novel Antimicrobial Peptides Derived from Flatfish Genes

Patrzykat

Gallant

Seo

et al. 2003

Antimicrob Agents Chemother

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We report on the identification of active novel antimicrobials determined by screening both the genomic information and the mRNA transcripts from a number of different flatfish for sequences encoding antimicrobial peptides, predicting the sequences of active peptides from the genetic information, producing the predicted peptides chemically, and testing them for their activities. We amplified 35 sequences from various species of flatfish using primers whose sequences are based on conserved flanking regions of a known antimicrobial peptide from winter flounder, pleurocidin. We analyzed the sequences of the amplified products and predicted which sequences were likely to encode functional antimicrobial peptides on the basis of charge, hydrophobicity, relation to flanking sequences, and similarity to known active peptides. Twenty peptides were then produced synthetically and tested for their activities against gram-positive and gram-negative bacteria and the yeast Candida albicans. The most active peptide (with the carboxy-terminus amidated sequence GWRTLLKKAEVK TVGKLALKHYL, derived from American plaice) showed inhibitory activity over a concentration range of 1 to 8 g/ml against a test panel of pathogens, including the intrinsically antibiotic-resistant organism Pseudomonas aeruginosa, methicillin-resistant Staphylococcus aureus, and C. albicans. The methods described here will be useful for the identification of novel peptides with good antimicrobial activities.

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“…Antibacterial activity was performed dominantly by antibacterial substances like antibacterial peptides abundant in the shrimp plasma whereas bacteriolytic activity was exerted mainly by some hydrolytic enzymes toward pathogens such as lysozyme. It has been proven that PS could interact with antibacterial peptide [41][42][43] and bind to lysozyme [44,45]. So we presumed that after injection of PS into shrimp, antibacterial and bacteriolytic substances could interact with PS so as to affect the biological activity of these enzymes, resulting in the distinct change of antibacterial and bacteriolytic activities in shrimp plasma.…”

Section: Discussionmentioning

confidence: 99%

Effects of phosphatidyl serine on immune response in the shrimp Litopenaeus vannamei

Yang

Pan

2013

Open Life Sciences

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Phosphatidyl serine plays an important role in animal innate immunity. Given its important functions, numerous investigations have been carried out on its immunological function in many animals. However, studies of phosphatidyl serine in the white shrimp Litopenaeus vannamei, an economically important animal, are rare. In this paper, we demonstrated influences of injecting phosphatidyl serine (PS) on immune response including some parameters from pro-phenol oxidase activating system (pro-PO system) and hemocyanin-derived phenol oxidase activity (Hd-PO) along with antibacterial and bacteriolytic activities in the white shrimp Litopenaeus vannamei with different PS concentrations (5, 10 and 20 μg mL−1). The results showed that PS could affect immune response of L. vannamei significantly (P<0.05), including total hemocyte counts (THC), PO activity from hemocyte, phenol oxidase (PO) activity from plasma, hemocyanin concentration, Hd-PO activity as well as antibacterial and bacteriolytic activities in the plasma. Among the lines, 20 μg mL−1 PS had the strongest effect on the above parameters, whereas 5 μg mL−1 had the least effect. The experimental results indicated that PS was able to activate exocytosis of pro-PO and formation of Hd-PO in white shrimp after injection, further regulating the immune process reflected by variation of antibacterial and bacteriolytic activities in a certain way.

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Interaction of pleurocidin and its analogs with phospholipid membrane and their antibacterial activity

Cited by 66 publications

References 35 publications

How did cells get their size?

How did cells get their size?

Novel Antimicrobial Peptides Derived from Flatfish Genes

Effects of phosphatidyl serine on immune response in the shrimp Litopenaeus vannamei

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