Novel Antimicrobial Peptides Derived from Flatfish Genes

Patrzykat, Aleksander; Gallant, Jeffrey W.; Seo, Jung-Kil; Pytyck, Jennifer; Douglas, Susan E.

doi:10.1128/aac.47.8.2464-2470.2003

Cited by 96 publications

(81 citation statements)

References 27 publications

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“…The substitutions were based on the circumstances of net charge, total hydrophobic ratio (%), positive charge distribution to arrange the hydrophobic residues on the same surface, amphiphilic character, and the proteinbinding potential (also known as the Boman index) (13). The sequences obtained after the residue substitutions were submitted to the predictive tool available at the Antimicrobial Peptide Database v2.34 (APD2; http://aps.unmc.edu/AP/main.php) (14) to verify their antimicrobial potential (15).…”

Section: Methodsmentioning

confidence: 99%

Prediction of Antimicrobial Activity of Synthetic Peptides by a Decision Tree Model

Lira

Perez

Baranauskas

et al. 2013

Appl Environ Microbiol

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Antimicrobial resistance is a persistent problem in the public health sphere. However, recent attempts to find effective substitutes to combat infections have been directed at identifying natural antimicrobial peptides in order to circumvent resistance to commercial antibiotics. This study describes the development of synthetic peptides with antimicrobial activity, created in silico by site-directed mutation modeling using wild-type peptides as scaffolds for these mutations. Fragments of antimicrobial peptides were used for modeling with molecular modeling computational tools. To analyze these peptides, a decision tree model, which indicated the action range of peptides on the types of microorganisms on which they can exercise biological activity, was created. The decision tree model was processed using physicochemistry properties from known antimicrobial peptides available at the Antimicrobial Peptide Database (APD). The two most promising peptides were synthesized, and antimicrobial assays showed inhibitory activity against Gram-positive and Gram-negative bacteria. Colossomin C and colossomin D were the most inhibitory peptides at 5 g/ml against Staphylococcus aureus and Escherichia coli. The methods described in this work and the results obtained are useful for the identification and development of new compounds with antimicrobial activity through the use of computational tools.

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Section: Methodsmentioning

confidence: 99%

Prediction of Antimicrobial Activity of Synthetic Peptides by a Decision Tree Model

Lira

Perez

Baranauskas

et al. 2013

Appl Environ Microbiol

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“…The 435 bp cDNA sequence contained an ORF of 168 bp that was predicted to encode a peptide of 55 amino acids, which consisted of signal peptide, mature peptide and pro-peptide. Through BLAST analysis and alignment, the full-length peptide had 87 percent similarity (70% identities) to Hb26 derived from Hippoglossus hippoglossus 17 and 80 percent similarity (59% identities) to PLE3 derived from Pseudopleuronectes americanus, 18 respectively (Figure 2). The physical and chemical property of the mature peptide compared with Hb26 and PLE3, as shown in Table 1.…”

Section: Sequence Analysismentioning

confidence: 99%

Identification, synthesis and characterization of a novel antimicrobial peptide HKPLP derived from Hippocampus kuda Bleeker

Sun

Jing

et al. 2012

J Antibiot

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A novel gene encoding 55 amino-acid residues has been identified from the brooding pouch cDNA library of Hippocampus kuda Bleeker. The deduced amino-acid sequence is highly homologous to several pleurocidin-like peptides from the winter flounder and comprises a signal peptide, a pro-peptide and a mature peptide. The glycine-rich mature peptide, designated HKPLP, contains 24 amino-acid residues and has been synthesized by solid-phase peptide synthesis. The purified HKPLP exhibits antimicrobial activity against several Gram-positive and Gram-negative bacterial strains at low concentrations (MIC 1.5-7.5 lM). Thermal stability assay data show good heat stability. CD spectroscopy experiments indicate that the dominant contents are anti-parallel and parallel sheets, which may have b-sheet or b-strand motif. It is inferred that HKPLP participates in the host defense during egg fertilization and embryo development as an antimicrobial peptide in brooding pouch.

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“…Using genomic screening, a gene family encoding pleurocidin, an AMP in the skin mucus secretions of winter flounder Pleuronectes americanus, was also identified in other flatfish, including yellowtail flounder Limanda ferruginea, Atlantic halibut Hippoglossus hippoglossus, American plaice Hippoglossoides platessoides and witch flounder Glyptocephalus cynoglossus, all of which are in the order Pleuronectiformes. A number of sequences from these fish were used to synthesize pleurocidin analogues, some of which had potent activity against a number of bacterial pathogens (Douglas et al 2001, Patrzykat et al 2003. However, Douglas et al (2001) were unable to detect the gene in fish outside this order, including haddock Melanogrammus aeglefinus, pollock Pollachius virens (both in the order Gadiformes) and smelt Osmerus mordax (order Salmoniformes).…”

Section: Detection Of Piscidins In Various Fish Speciesmentioning

confidence: 99%

“…Douglas et al (2001) and Patrzykat et al (2003) used this approach to identify homologous sequences of pleurocidin in various fish. However, genomic approaches such as in situ hybridization using oligonucleotide probes to locate related peptides have significant limitations since even if the amino acid sequences are highly similar, different species of fish may use different codons for the same amino acid, making it difficult to design probes that will recognize similar proteins.…”

Section: Detection Of Piscidins In Various Fish Speciesmentioning

confidence: 99%

Evidence for widespread distribution of piscidin antimicrobial peptides in teleost fish

Silphaduang¹,

Colorni²,

Noga³

2006

Dis. Aquat. Org.

161

126

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Antimicrobial peptides (AMPs) are increasingly recognized as a critical component of the host's defense against infection. Several types of AMPs have been recently identified from mucosal tissues or immune cells of a number of teleosts. Among these are the piscidins, which are 22 residue, α-helical AMPs that were originally isolated from mast cells of hybrid striped bass Morone saxatilis male × Morone chrysops female. Using an antibody specific for the conserved N-terminal amino acid sequence of piscidin 1, we used immunohistochemistry to probe skin, gill, and gastrointestinal tract of 39 teleosts representing 7 different orders. Nine fish species were piscidin-positive, with all of these species being in the Perciformes, the largest and most evolutionarily advanced order of teleosts. Piscidin-positive cells were identified in species belonging to the families Moronidae, Serranidae, Sciaenidae, Siganidae and Belontidae. Immunopositive cells were usually most consistent with mast cells, although in some species, the granule appearance and tinctorial properties diverged somewhat from those of a typical piscine mast cell. In addition, rodlet cells were piscidin-positive in one member of the family Cichlidae; to our knowledge, it is the first time that a host-associated chemical biomarker has been identified in rodlet cells. Our data suggest that piscidins are present in many evolutionarily advanced teleosts. Piscidin-immunoreactive cells were most common at sites of pathogen entry, including the skin, gill and gastrointestinal tract. These results strongly suggest that piscidins are a widespread and important component of many fishes' defense against disease. KEY WORDS: Piscidins · Mast cells · Rodlet cells · Innate immunity · Pisces Resale or republication not permitted without written consent of the publisherDis Aquat Org 72: [241][242][243][244][245][246][247][248][249][250][251][252] 2006 tissues of hybrid striped bass; the parental stocks of hybrid striped bass (white bass Morone chrysops and striped bass M. saxatilis) are in the family Moronidae, and similar appearing cells were positive in members of the family Sciaenidae (spot Leistomus xanthurus and Atlantic croaker Micropogonias undulatus). This prompted us to hypothesize that piscidins are present in other teleosts. To answer this question, we immunochemically examined tissues from 7 different orders of teleosts. Presumptively healthy, subadult to adult, farm-raised fish, as well as wild-caught individuals, were examined in the present study. MATERIALS AND METHODSPeptide synthesis. Piscidin 1 (both the entire peptide and an 11-mer fragment) and Magainin 2 peptides were synthesized using Fmoc chemistry on a Rainin Symphony instrument that provides on-instrument cleavage of the peptide from the resin. After synthesis, peptides were purified via analytical reverse phase HPLC using a YMC C-18 column (4 × 50 mm, 3 µm particle size, 120 Å pore size support) using an acetonitrile gradient that was eluted at 1 ml min -1 where Buffer A was 0.05% trif...

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Novel Antimicrobial Peptides Derived from Flatfish Genes

Cited by 96 publications

References 27 publications

Prediction of Antimicrobial Activity of Synthetic Peptides by a Decision Tree Model

Prediction of Antimicrobial Activity of Synthetic Peptides by a Decision Tree Model

Identification, synthesis and characterization of a novel antimicrobial peptide HKPLP derived from Hippocampus kuda Bleeker

Evidence for widespread distribution of piscidin antimicrobial peptides in teleost fish

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