Interaction of plasma proteins with surfaces

Fenstermaker, Carl A.; Grant, Warren H.; Morrissey, B. W.; Smith, Leslie E.; Stromberg, Robert R.

doi:10.6028/nbs.ir.74-470

Cited by 16 publications

(13 citation statements)

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“…As discussed more fully elsewhere, ellipsometry permits an estimation of the thickness and density of an adsorbed film. 6 This tool has been used by several groups of investigators for the study of protein adsorption onto a variety of materials.2.6J1 Al-though the interpretation of ellipsometric data requires the solution of a transcendental equation and as such makes difficult the continuous collection of data, an investigation of time-dependent desorption phenomena using a recording ellipsometer has been reported." I n addition, ellipsometry has proven useful for the qualitative study of interactions between proteins in solution and those preadsorbed onto a surface.…”

Section: Introductionmentioning

confidence: 99%

A total internal‐reflection technique for the examination of protein adsorption

Watkins

Robertson

1977

J. Biomed. Mater. Res.

117

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A total internal-reflection fluorescence (TIRF) technique for examining protein adsorption at solid-liquid interfaces is described. For the representative case of adsorption of bovine gamma-globulin onto silicone rubber, the applicability of the technique to serveral important aspects of protein adsorption phenomena is demonstrated. Specifically, in addition to the tightly adsorbed protein layer observed herein and previously by maany others, the presence of a loosely adsorbed protein layer was also noted under conditions of either static or flowing protein solutions. Taking advantage of the continuous real-time measurements possible with TIRF, a rate constant for the protein adsorption step during laminar flow was estimated at both 25 and 37 degrees C. These results serve to demonstrate that the TIRF technique represents a significant and versatile new tool for the study of protein adsorption phenomena.

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Section: Introductionmentioning

confidence: 99%

A total internal‐reflection technique for the examination of protein adsorption

Watkins

Robertson

1977

J. Biomed. Mater. Res.

117

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“…On high-yc materials, proteins are deformed and segments exposed to red cell contact which are normally not exposed when the protein is free in the plasma or held to a low-y surface (36)(37)(38). On high-yc materials, proteins are deformed and segments exposed to red cell contact which are normally not exposed when the protein is free in the plasma or held to a low-y surface (36)(37)(38).…”

Section: Discussionmentioning

confidence: 99%

Material Effects in Shear-Induced Hemolysis

Offeman

Williams

1979

Biomaterials, Medical Devices, and Artificial Organs

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A rotating-disk apparatus for shearing blood was employed to compare 22 materials for their tendency to cause hemolysis during standardized low-stress (130 dynes/cm2 maximum) laminar flow conditions. Rigid plastics, hydrogels, and carbons were among the materials tested. Time-independent ranking of the materials was possible for 75% of the data; these rankings correlated linearly (for polymers) with the critical surface tension gammac over the range 20--46 dyne/cm, with low gammac being associated with low hemolysis. Surface morphology was also found to influence hemolysis. Roughness in the 1--15 micron range had a distinct effect on hemolysis kinetics for polyethylene surfaces. This suggest that failure to find time-independence in 25% of the material rankings can be attributed to inadequate control of the roughness variable. It also emphasizes the importance of surface morphology, as well as surface chemistry, as a biocompatibility parameter.

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“…The findings of partial displacement and mixed monolayer formation on octadecylsilylated glass appear in accordance with previous ellipsometric studies on glass.20 Interestingly, other workers have reported that fibrinogen can totally displace a monolayer of BSA adsorbed on silica. 21 When the attacking species was the amphiphilic macromolecule GP, ready penetration of both the BSA and HS monolayers occurred ( Figs. 1 and 2), yielding mixed monolayers that showed essentially the same { versus pH profiles as those obtained by exposing GP monolayers to BSA or HS (Fig.…”

Section: Discussionmentioning

confidence: 99%

The interaction of macromolecular solutions with macromolecular monolayers adsorbed on a hydrophobic surface

Fromageot

Groves

Sears

1976

J. Biomed. Mater. Res.

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In order to elucidate the general patterns of intermacromolecular surface interactions that may be involved in hemocompatibility phenomena, monolayers of representative macromolecules on an octadecylsilylated glass surface were exposed to solutions of other macromolecules, and the changes in interfacial composition were characterized by zeta potential-pH titration curves, as measured by alternating flow streaming current analysis and, in some cases, by radiotracer labeling. Experiments with poly(vinylpyrrolidone) (PVP), a blood-compatible linear polymer; bovine serum albumin (BSA), a representative serum protein; whole human serum (HS), a complex mixture of proteins; and erythrocyte surface glycoprotein (GP), an extended-chain macromolecular amphiphile, showed the following: 1) Penetration of the original monolayer occurred within 24 hr in 9 of the 12 possible cases; it did not occur for BSA or HS monolayers exposed to PVP, and probably not for PVP exposed to GP. 2) In all cases, penetration was accompanied by no more than partial displacement of the original monolayer, thereby generating a mixed monolayer. Each of the six possible binary mixed monolayers could be obtained by at least one of the two possible mixing sequences. 3) In the three binary systems containing BSA, the formation of the mixed monolayer could be related to increased adsorption in the two-component system. 4) The two components of the mixed monolayers were not equally distributed across their thicknesses: thus, the outer surfaces of the PVP-BSA and (at neutral pH) the PVP-HS mixed monolayers contained only PVP; that of the BSA-HS mixtures only HS. In the PVP-HS, and probably the GP-BSA and GP-HS mixed monolayers, the composition of the outer surface appeared pH-dependent. The resultant zeta potential versus pH profiles in the latter two cases resembled those of intact blood cells. The results suggest that neither the compact monolayers of globular proteins nor the diffuse monolayers of randomly coiled water-soluble polymers can, by their prior adsorption on a synthetic surface, prevent the subsequent adsorption of other globular macromolecules. It is possible that the randomly coiled polymers may impede the adhesion of platelets to the substrate since the results indicate that the adsorption of such polymers causes a displacement of the shear plane.

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Interaction of plasma proteins with surfaces

Cited by 16 publications

References 0 publications

A total internal‐reflection technique for the examination of protein adsorption

A total internal‐reflection technique for the examination of protein adsorption

Material Effects in Shear-Induced Hemolysis

The interaction of macromolecular solutions with macromolecular monolayers adsorbed on a hydrophobic surface

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