Interaction of monoclonal antibodies with a neurite outgrowth factor from chicken gizzard extract

Hayashi, Yokichi; Taniura, Hideo; Miki, Naomasa

doi:10.1016/0165-3806(87)90003-4

Cited by 14 publications

(4 citation statements)

References 25 publications

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“…We have reported that NOF binding protein identified in this way is present in chick gizzard muscles and that an 82-kD glycoprotein solubilized from chicken gizzard muscles is a possible NOF receptor (19). Neurite extension from CG or retinal neurons induced by NOF depends strictly on the developmental stage (8,11). The neuritic response of these neurons to NOF ismaximal in 6-10-d embryos and thereafter decreases gradually.…”

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Purification and characterization of an 82-kD membrane protein as a neurite outgrowth factor binding protein: possible involvement of NOF binding protein in axonal outgrowth in developing retina.

Taniura

Kuo

Hayashi

et al. 1991

The Journal of Cell Biology

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Abstract. Neurite outgrowth factor (NOF) is a glycoprotein isolated from an extract of gizzard that induces neurite outgrowth from cultured retinal or ciliary gan. glionic (CG) neurons. We have reported that a glycoprotein of •82 kD solubilized from gizzard muscles binds to NOF (ligand blotting) and inhibits the neurite promoting activity of NOF (inhibition assay). The 82-kD protein (NOF binding protein) was purified from gizzard muscle membranes as a doublet band on SDS-PAGE and a polyclonal antibody was raised against it. An NOF binding protein in developing retina exhibited the same physicochemical properties as that of the gizzard muscle. Quantitative decrease in NOF binding protein in embryonic retinas was observed after day 11 by the inhibition assay, ligand blotting, and immunoblotting, its decrease being parallel with reduction of NOF-induced neurite outgrowth of embryonic retinas. In an immunohistochemical study,, the antibody stained only the optic fiber layers of the retinas of 8-d embryos, and this staining was no longer detectable in retinas of 18-d embryos. These results suggest that the 82-kD protein is a novel membrane protein that behaves as an NOF receptor and that the loss of neuritic response of the retinal neurons to NOF reflects a decrease in NOF receptor molecules.

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confidence: 82%

“…The methods used for preparations of polyclonal and monoclonal (1-4D) antibodies against NOF were described previously (6,8). Immunoblotting onto a nitrocellulose shoot was also carried out as described previously (6).…”

Section: Immunoblotting and Ligand Blottingmentioning

confidence: 99%

Purification and characterization of an 82-kD membrane protein as a neurite outgrowth factor binding protein: possible involvement of NOF binding protein in axonal outgrowth in developing retina.

Taniura

Kuo

Hayashi

et al. 1991

The Journal of Cell Biology

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“…Gicerin was first identified as a cellular receptor of neurite outgrowth factor (NOF) (Taniura et al . 1991), an extracellular matrix glycoprotein belonging to the laminin family that exhibits strong neurite promoting activity (Hayashi and Miki 1985; Hayashi et al . 1987; Kato et al .…”

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Expression and involvement of gicerin, a cell adhesion molecule, in the development of chick optic tectum

Taira

Tsukamoto

Kohama

et al. 2004

Journal of Neurochemistry

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Gicerin is a cell adhesion molecule belonging to the immunoglobulin superfamily. It has both a homophilic binding activity and a heterophilic binding activity to neurite outgrowth factor (NOF) a molecule belonging to the laminin family. We have reported many studies on the heterophilic activity of gicerin and NOF, but the function of its homophilic binding activity in vivo had been unclear. In the retina, gicerin is expressed in retinal ganglion cells only when they extend neurites to the optic tectum. In this report we have found that gicerin is also transiently expressed in the optic tectum during this time. First, cell aggregation assays were used to show that gicerin expressed in the optic tectum displays homophilic binding activity. Then, explant cultures of embryonic day 6 chick optic tectum on gicerin-Fc chimeric protein-coated dishes and NOF-coated dishes were carried out. It was found that gicerin-gicerin homophilic interactions promoted cell migration, whereas heterophilic interactions with NOF induced neurite formation. Furthermore, when anti-gicerin antibodies were injected in order to examine the effect of gicerin protein in the formation of the tectal layer in ovo, cell migration was strongly inhibited. These data suggest that homophilic interaction of gicerin participates in the migration of neural cells during the layer formation and plays a crucial role in the organization of the optic tectum.

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“…Basic fibroblast growth factor (basic FGF) was also reported to show neurite-promoting activity on several types of embryonic neurons in culture (Momson et al, 1986;Walicke et al, 1986;Unsicker et al, 1987). In addition to these factors, unidentified neurite-promoting factors have been reported (Varon et al, 1983/84;Gahwiler and Hefti, 1984;Muller et al, 1984;Hayashi et al, 1987;Rauvala et al, 1987;Sandrock and Matthew, 1987).…”

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Neurite‐Promoting Activities of Phosphatidylinositol and Other Lipids on Fetal Rat Septal Neurons in Culture

Arakawa

Isahara

Tachibana

1991

Journal of Neurochemistry

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Neurite-promoting activities of lipids were assessed using serum-free cultures of fetal rat septal neurons. The most potent one was phosphatidylinositol (PI), followed by PI 4-phosphate, phosphatidylserine, sphingomyelin, and phosphatidylcholine. The EC50 value for PI was 1.5 micrograms/ml (1.8 microM), and activity was maximal at 4 micrograms/ml (56% of total cells had neurites after 24 h). Cerebroside, sulfatide, and di- and triacylglycerols showed relatively low activities. Synthetic dipalmitoyl phosphatidylcholine was also active, with a maximal activity (47%) at 100 micrograms/ml, a finding implying that the unsaturated fatty acid moiety is not released and further used as substrate for the arachidonic acid cascade. Lysophospholipids, phosphatidylglycerol, and cardiolipin were rather cytotoxic and lacked activity, an observation suggesting that membrane perturbation is not responsible for the neurite-promoting activity. Treatment with a protein kinase C inhibitor, H-7, or an Na+,K(+)-ATPase inhibitor, ouabain, inhibited the PI-induced neurite outgrowth, but the cyclic AMP- and cyclic GMP-dependent protein kinase inhibitor HA1004 did not inhibit this activity, a result indicating that multiple elements (protein kinase C and Na+,K(+)-ATPase) are involved in the induction of neurites. Because phospholipids can be provided either as lipid vesicles or as lipoproteins produced by macrophages at regeneration sites, they may play an important role in the regeneration of certain populations of neurons.

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Interaction of monoclonal antibodies with a neurite outgrowth factor from chicken gizzard extract

Cited by 14 publications

References 25 publications

Purification and characterization of an 82-kD membrane protein as a neurite outgrowth factor binding protein: possible involvement of NOF binding protein in axonal outgrowth in developing retina.

Purification and characterization of an 82-kD membrane protein as a neurite outgrowth factor binding protein: possible involvement of NOF binding protein in axonal outgrowth in developing retina.

Expression and involvement of gicerin, a cell adhesion molecule, in the development of chick optic tectum

Neurite‐Promoting Activities of Phosphatidylinositol and Other Lipids on Fetal Rat Septal Neurons in Culture

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