Purification and characterization of an 82-kD membrane protein as a neurite outgrowth factor binding protein: possible involvement of NOF binding protein in axonal outgrowth in developing retina.

Taniura, Hideo; Kuo, Che‐Hui; Hayashi, Yokichi; Miki, Naomasa

doi:10.1083/jcb.112.2.313

Cited by 30 publications

(12 citation statements)

References 25 publications

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“…We purified gicerin, a membrane protein from chicken gizzard smooth muscle, as a neurite promotion factor (Taniura et al 1991). This molecule is a cell adhesion molecule in the Ig superfamily and has a homophilic adhesion property (gicerin-gicerin) and a heterophilic adhesive manner in regard to the neurite outgrowth factor (NOF), an extracellular matrix protein in the laminin family (Hayashi and Miki 1985;Taira et al 1994).…”

Section: Introductionmentioning

confidence: 99%

Gicerin, a cell adhesion molecule, promotes the metastasis of lymphoma cells of the chicken

Tsuchiya

Tsukamoto

Furuya

et al. 2003

Cell and Tissue Research

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Gicerin is an immunoglobulin superfamily cell adhesion molecule purified from chicken gizzards. This molecule displays an adhesive interaction with a laminin-like protein as well as with gicerin itself. Gicerin appears in embryonic tissues and plays a role in chick development through its cell adhesive properties. An increase in gicerin expression is found in some sporadic tumors of the chicken. To elucidate the possible role of gicerin in tumor progression in chickens, we introduced gicerin cDNA into an endogenous gicerin negative lymphoma MDCC-MSB1 cell line, and subsequently analyzed them for changes in their metastatic potentials. After intravenous implantation of the gicerin transfectants into chickens, the metastatic potential to the lung, liver and kidney was enhanced compared with parental MDCC-MSB1 cells. Self-aggregation activity was increased in gicerin transfectants. In addition, adhesive and migratory activities of the gicerin transfectants to the gicerin ligands were enhanced in vitro. These findings indicate that gicerin can contribute to the malignancy and metastatic properties of lymphoma.

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Section: Introductionmentioning

confidence: 99%

Gicerin, a cell adhesion molecule, promotes the metastasis of lymphoma cells of the chicken

Tsuchiya

Tsukamoto

Furuya

et al. 2003

Cell and Tissue Research

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“…Differential expression among cell types may help account for different responses to NOF and gicerin seen in these regions. We have reported that neurons in cultured retinal explants that express s-gicerin selectively extend neurites in response to NOF (29), while the cerebellar explants cultured on NOF display migration of neurons (probably granular cells) in addition to neurite extension (30).…”

Section: Discussionmentioning

confidence: 99%

“…Gicerin was originally purified from chicken gizzard as a doublet with molecular sizes of 82 and 90 kDa (29). Recently, we have reported the molecular cloning and functional analysis of gicerin, which we renamed s-gicerin here (27).…”

Section: Discussionmentioning

confidence: 99%

“…They were treated with 0.05% trypsin and 0.53 mM EDTA for 20 min at room temperature, then resuspended in Dulbecco's modified Eagle's medium containing 10% FCS and dissociated by gentle pipetting. Resultant single cells were preincubated on a plastic dish for 1 h to let non-neuronal cells attach to the dish (29,38). The nonattached ciliary ganglion neurons were plated on feeder layers of confluent gicerin transfectants or parental L929 cells grown in 30-mm dishes (ϳ500 CG cells/dish) and co-incubated for 24 h (39).…”

Section: Neurite Extension Of Ciliary Ganglion Cells On the Feeder Lamentioning

confidence: 99%

“…It was first identified as a binding protein for neurite outgrowth factor (NOF), a member of the laminin family (28,29). In the nervous system gicerin expression is transient, restricted to early stages of development when neurons extend neurites or undergo migration (30), whereas constitutive expression is found in muscles and kidney.…”

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Expression and Functional Analysis of a Novel Isoform of Gicerin, an Immunoglobulin Superfamily Cell Adhesion Molecule

Taira¹,

Nagino²,

Taniura³

et al. 1995

Journal of Biological Chemistry

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We have cloned a novel cDNA of gicerin, a cell adhesion molecule belonging to the immunoglobulin superfamily. Both gicerin isoforms share the same extracellular domain, which has five immunoglobulin-like loop structures and a transmembrane domain as s-gicerin, but differ in the cytoplasmic tail domain. As the newly identified form has a larger cytoplasmic domain than the previously reported form, we refer to them as l-gicerin and s-gicerin, respectively. l-gicerin is transcribed from a distinct mRNA containing an inserted sequence not found in s-gicerin mRNA which caused a frameshift for the coding region for a cytoplasmic domain. Previous studies demonstrated that gicerin showed a doublet band of 82 and 90 kDa in chicken gizzard smooth muscle. We report that the 82-kDa protein corresponds to s-gicerin and the 90-kDa protein to l-gicerin. We also found that the two gicerin isoforms are expressed differentially in the developing nervous system. Functional analysis of these gicerin isoforms in stable transfectants revealed that they had differ in their homophilic adhesion properties, as well as in heterophilic cell adhesion assayed with neurite outgrowth factor. In addition, these isoforms have neurite-promoting activity by their homophilic adhesion, but differ in their ability to promote neurite outgrowth.

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Gicerin, a cell adhesion molecule, participates in the histogenesis of retina

et al. 1997

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Gicerin is a novel cell adhesion molecule that belongs to the immunoglobulin superfamily. Gicerin protein adheres to neurite outgrowth factor (NOF), an extracellular matrix protein in the laminin family, and also exhibits homophilic adhesion. Heterophilic adhesion of gicerin to NOF is thought to play an active role in neurite outgrowth of developing retinal cells in vitro. In this study, we examined the adhesion activity of gicerin during the retinal development of Japanese quail using an antibody directed against gicerin, to elucidate the biological importance of gicerin in retinal histogenesis. Immunohistochemical and Western blot analysis showed that gicerin was highly expressed in the developing retina but suppressed in the mature retina. The aggregation of neural retinal cells from 5‐day embryonic quail retina was significantly inhibited when incubated with a polyclonal antibody to gicerin, suggesting that gicerin protein participates in the adhesion of neural retinal cells of the developing retina. Furthermore, histogenesis of retina both in the organ cultures and in ovo embryos was severely disrupted by incubation with a gicerin antibody. These findings provide evidence that gicerin plays an important role in retinal histogenesis. © 1997 John Wiley & Sons, Inc. J Neurobiol 33: 769–780, 1997

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Purification and characterization of an 82-kD membrane protein as a neurite outgrowth factor binding protein: possible involvement of NOF binding protein in axonal outgrowth in developing retina.

Cited by 30 publications

References 25 publications

Gicerin, a cell adhesion molecule, promotes the metastasis of lymphoma cells of the chicken

Gicerin, a cell adhesion molecule, promotes the metastasis of lymphoma cells of the chicken

Expression and Functional Analysis of a Novel Isoform of Gicerin, an Immunoglobulin Superfamily Cell Adhesion Molecule

Gicerin, a cell adhesion molecule, participates in the histogenesis of retina

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