Interaction of Liposomes with Blood Plasma Proteins

Black, Christopher D. V.; Gregoriadis, Gregory

doi:10.1042/bst0040253a

Cited by 95 publications

(15 citation statements)

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“…Liposomes are injectable but may interact with blood proteins (Black and Gregoriadis, 1976). A recent study (Kibat et al, 1990) shows that enclosing liposomes in calcium alginate beads seems to protect the liposomes from reactions within the body.…”

Section: Discussionmentioning

confidence: 98%

Calcium alginate beads as a slow‐release system for delivering angiogenic molecules In Vivo and In Vitro

Downs¹,

Robertson²,

Riss³

et al. 1992

Journal Cellular Physiology

139

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A method previously used in this laboratory for entrapment of tumor cells in alginate beads has been extended to provide a slow release delivery system for growth factors with known in vivo angiogenic activity. Protein growth factors were entrapped in alginate beads in amounts sufficient to cause incorporation of 3H-thymidine by COMMA-D cells in vitro, and in vivo neovascularization when injected subcutaneously into Balb/c mice. Entrapment of 125I-labelled growth factors showed that the amount of molecule entrapped in alginate beads may vary with the charge of the molecule. In vitro cell proliferation studies showed that entrapment in alginate beads may provide a slow-release system or a stabilizing environment for the protein. In some cases biological activity of the growth factor in solution was increased by the presence of control alginate beads. When alginate-entrapped growth factors were injected into Balb/c mice, induction of new blood vessels could be monitored qualitatively by macroscopic photography and assessed quantitatively by measuring the pooling of radiolabelled red blood cells at the experimental site. Subcutaneous injection of purified angiogenic factors not entrapped in alginate beads did not cause neovascularization. Diffusion of 125I-labelled growth factors from alginate beads in the animal showed that release in vivo may depend on the charge of the protein molecule. These results indicate that injection of purified molecules entrapped in alginate beads provides an effective localized and slow-release delivery of biologically active molecules. This delivery system may extend the time of effectiveness of biologically active molecules in vivo compared to direct injection without alginate entrapment. The method of entrapment and injection has potential for identifying active factors in tumor-induced angiogenesis and testing new compounds as modulators of neovascularization.

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Section: Discussionmentioning

confidence: 98%

Calcium alginate beads as a slow‐release system for delivering angiogenic molecules In Vivo and In Vitro

Downs¹,

Robertson²,

Riss³

et al. 1992

Journal Cellular Physiology

139

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“…This interpretation is supported by a study that demonstrated the reversal of a net positive charge on cationic liposomes to net negative after incubation in sera, indicating extensive interaction with serum proteins. 47 Other studies indicate that higher concentrations of sera are required to inhibit the cellular interaction of cationic liposomes lacking asODN than are required to inhibit the interaction when asODN were present in the formulation. 15 Neutral liposomes containing passively entrapped 125 IasODN avoid uptake into MPS tissues to a greater extent than cationic liposomes.…”

Section: Discussionmentioning

confidence: 99%

A novel, long-circulating, and functional liposomal formulation of antisense oligodeoxynucleotides targeted against MDR1

2000

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The goal of this study was to develop a small, stable liposomal carrier for antisense oligodeoxynucleotides (asODN) that would have high trapping efficiencies and long circulation times in vivo. Traditional cationic liposomes aggregate to large complexes and, when injected intravenously, rapidly accumulate in the liver and lung. We produced charge-neutralized liposome-asODN particles by optimizing the charge interaction between a cationic lipid and negatively charged asODN, followed by a procedure in which a layer of neutral lipids coated the exterior of the cationic lipid-asODN particle. The coated cationic liposomes had an average diameter of 188 nm and entrapped 85-95% of the asODN. The biodistribution and pharmacokinetics of an 18-mer 125 I-labeled phosphorothioate ODN formulated by this method were determined after tail vein injection in mice. The majority of the asODN was cleared from blood, with a half-life of Ͼ10 hours compared with Ͻ1 hour for free asODN. When coupled with an anti-CD19 targeted antibody, this formulation was also effective at delivering an MDR1 asODN to a multidrug-resistant human B-lymphoma cell line in vitro, decreasing the activity of P-glycoprotein. No inhibition was found for nontargeted formulations or for free asODN. A number of therapeutic opportunities exist for the use of small, stable, long-circulating, and targetable liposomal carriers such as this, with high trapping efficiencies for asODN. Cancer Gene Therapy (2000) 7, 466 -475

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“…The literature has described that charged particles, particularly cationic ones, are generally strong activators of the immune system (38,39). Any of several attractive forces including Van der Walls, electrostatic, ionic and hydrogen bonds can be involved in opsonin binding to the NP + surface (40). The second is the presence of a polysaccharide backbone including many hydroxyl (OH) groups.…”

Section: Complement Activationmentioning

confidence: 99%

Positively-Charged, Porous, Polysaccharide Nanoparticles Loaded with Anionic Molecules Behave as ‘Stealth’ Cationic Nanocarriers

et al. 2009

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Interaction of Liposomes with Blood Plasma Proteins

Cited by 95 publications

References 0 publications

Calcium alginate beads as a slow‐release system for delivering angiogenic molecules In Vivo and In Vitro

Calcium alginate beads as a slow‐release system for delivering angiogenic molecules In Vivo and In Vitro

A novel, long-circulating, and functional liposomal formulation of antisense oligodeoxynucleotides targeted against MDR1

Positively-Charged, Porous, Polysaccharide Nanoparticles Loaded with Anionic Molecules Behave as ‘Stealth’ Cationic Nanocarriers

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