Interaction of Pseudomonas Bacteriophage 2 with the Slime Polysaccharide and Lipopolysaccharide of Pseudomonas aeruginosa Strain BI

Abstract

Purified slime polysaccharide B and lipopolysaccharide of Pseudomonas aeruginosa strain BI were shown to possess receptor-like properties in inactivating Pseudomonas phage 2, whereas lipoprotein and glycopeptide fractions were devoid of activity. On a weight basis, slime polysaccharide B was more effective than lipopolysaccharide in inactivating phage. The specificity of the reaction with slime polysaccharide B was indicated by the fact that slime polysaccharide A of P. aeruginosa strain El failed to inactivate phage 2. Electron micrographs showed phage 2 in typical, tail-first position of attachment on intact cells of strain BI, slime polysaccharide B, and lipopolysaccharide. Tail fibers were discernible during phage attachment. The concept of specific phage receptor sites in the bacterial cell wall is well established. Inactivation of phages by isolated cell walls and purified receptor components of the cell wall has been

Phage-related surface modifications of Pseudomonas aeruginosa: effects on the biological activity of viable cells

“…The infection of various strains of P. aeruginosa with pseudomonas phages, including phage 8, is accompanied by the synthesis of a depolymerase that appears to be under the direction of the phages (4). Furthernore, the surface substrate for the depolymerase, located in the GLP fraction, may also contribute to the initial adsorption of the phages (7). The present study supported these hypotheses: mutants of the wild-type strain EI that lacked phage 8 receptors also lacked available substrate for the phageassociated depolymerase.…”

“…Origin of phage 8 and its depolymerase. The attachment of phage 8 to P. aeruginosa strain EI and the phage-receptor properties of the slime GLP layers of strains BI and EI have been reported (4,7). Strain 1234 (8), which carries phage 8 as a prophage, provided the opportunity to observe the kinetics of depolymerase synthesis relative to infective phage replication.…”

Bacteriophage Receptors, Mechanisms of Phage Adsorption and Penetration into Host Cell

“…Position of a receptor in O-chain of S-type of LPS is described for phage 2. This bacteriophage infecting wild type strain of Pseudomonas aeruginosa B1 is affiliated to group B according to Bradley classification and to siphoviridae family according to modern classification because it has a long expanded tail (Bartell et al, 1971). This phage displays depolymerase activity owing to the constituent enzyme.…”

Phages of Pseudomonas aeruginosa: response to environmental factors and in vitro ability to inhibit bacterial growth and biofilm formation

“…The EPS were resuspended in 70% ethanol, centrifuged, air dried and finally freeze-dried. For LPS isolation, previously prepared and lyophilized host cell walls were treated by hot phenol (45%) at 74°C for 20 min (Bartell et al 1971). Following the procedure, the mixture was subsequently cooled on ice, centrifuged (2000 g, 1 h at 4°C), the aqueous phase was dialysed for 4 days at 4°C and lyophilized.…”