Interaction of gold nanoparticles with free radicals and their role in enhancing the scavenging activity of ascorbic acid

Razzaq, Humaira; Saira, Farhat; Yaqub, Azra; Qureshi, Rumana; Mumtaz, Muhammad; Saleemi, Samia

doi:10.1016/j.jphotobiol.2016.04.003

Cited by 31 publications

(12 citation statements)

References 34 publications

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“…On the other hand, the majority of reports confirm that AuNPs has the ability to scavenge the DPPH free radical, in consistent with our experimental result (S1 Fig) [46, 102–105]. This scavenging power is attributed to the DPPH adsorption and complex formation on AuNPs surface, which indicates the displacement of some citrate ions when sodium citrate is used in reduction [104]. In the newly synthesized nanocomposite CAG herein, it is expected that both CR and AuNPs exert radical inhibition in a multi-potent model, where CR plays the major role by its high reducing power and phenolic content, which facilitate H-donation from phenolic–OH groups to DPPH reagent [89].…”

Section: Resultssupporting

confidence: 91%

Hybrid nanocomposite curcumin-capped gold nanoparticle-reduced graphene oxide: Anti-oxidant potency and selective cancer cytotoxicity

et al. 2019

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Nanotechnology-based antioxidants and therapeutic agents are believed to be the next generation tools to face the ever-increasing cancer mortality rates. Graphene stands as a preferred nano-therapeutic template, due to the advanced properties and cellular interaction mechanisms. Nevertheless, majority of graphene-based composites suffer from hindered development as efficient cancer therapeutics. Recent nano-toxicology reviews and recommendations emphasize on the preliminary synthetic stages as a crucial element in driving successful applications results. In this study, we present an integrated, green, one-pot hybridization of target-suited raw materials into curcumin-capped gold nanoparticle-conjugated reduced graphene oxide (CAG) nanocomposite, as a prominent anti-oxidant and anti-cancer agent. Distinct from previous studies, the beneficial attributes of curcumin are employed to their fullest extent, such that they perform dual roles of being a natural reducing agent and possessing antioxidant anti-cancer functional moiety. The proposed novel green synthesis approach secured an enhanced structure with dispersed homogenous AuNPs (15.62 ± 4.04 nm) anchored on reduced graphene oxide (rGO) sheets, as evidenced by transmission electron microscopy, surpassing other traditional chemical reductants. On the other hand, safe, non-toxic CAG elevates biological activity and supports biocompatibility. Free radical DPPH inhibition assay revealed CAG antioxidant potential with IC 50 (324.1 ± 1.8%) value reduced by half compared to that of traditional citrate-rGO-AuNP nanocomposite (612.1 ± 10.1%), which confirms the amplified multi-potent antioxidant activity. Human colon cancer cell lines (HT-29 and SW-948) showed concentration- and time-dependent cytotoxicity for CAG, as determined by optical microscopy images and WST-8 assay, with relatively low IC 50 values (~100 μg/ml), while preserving biocompatibility towards normal human colon (CCD-841) and liver cells (WRL-68), with high selectivity indices (≥ 2.0) at all tested time points. Collectively, our results demonstrate effective green synthesis of CAG nanocomposite, free of additional stabilizing agents, and its bioactivity as an antioxidant and selective anti-colon cancer agent.

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Section: Resultssupporting

confidence: 91%

Hybrid nanocomposite curcumin-capped gold nanoparticle-reduced graphene oxide: Anti-oxidant potency and selective cancer cytotoxicity

et al. 2019

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“…Ma et al () confirmed what GNPs inhibit NO production and iNOS gene expressions by suppressing NF‐kB activity by blocking the degradation of IkBa in an inflammatory model. Moreover, large positively charged surface area of GNPs leads to interaction between the unpaired electrons of free radicals and protons present on surfaces of nanoparticles (Barathmanikanth et al, ; Chen et al, ; Karthick et al, ; Razzaq et al, ; Silveira et al, ), which stabilize these molecules.…”

Section: Discussionmentioning

confidence: 99%

“…In the present study, treatment with GNPs reduced levels of markers of oxidant production and oxidative damage. According to Razzaq et al (), GNPs and other noble metal nanoparticles exhibit high catalytic activity mediating free radical scavenging reactions. To evaluate oxidant effects of GNPs, SOD, and CAT activities and total GSH levels were analyzed.…”

Section: Discussionmentioning

confidence: 99%

Effects of treatment with gold nanoparticles in a model of acute pulmonary inflammation induced by lipopolysaccharide

Haupenthal

Mendes

Silveira

et al. 2019

J Biomedical Materials Res

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The bacterial lipopolysaccharide (LPS) is a highly toxic molecule derived from the outer membrane of gram-negative bacteria. LPS endotoxin affects the lungs and is used as a model of acute pulmonary inflammation affecting the cellular morphology of the organ. Previously, gold nanoparticles (GNPs) have been shown to demonstrate anti-inflammatory and antioxidative activity in muscle and epithelial injury models.The objective of this study was to investigate the effect of the intraperitoneal treatment using GNPs on the inflammatory response and pulmonary oxidative stress induced by LPS. Wistar rats were divided into four groups (N = 10): Sham; Sham + GNPs 2.5 mg/kg; LPS; and LPS + GNPs 2.5 mg/kg. Treatment with LPS upregulated the levels of markers of cellular and hepatic damage (CK, LDH, AST, and alanine aminotransferase); however, the group treated with only GNPs exhibited no toxicity.Treatment with GNPs reversed LPS-induced changes with respect to total peritoneal leukocyte count and the pulmonary levels of pro-inflammatory cytokines (IFN-γ and IL-6). Histological analysis revealed that treatment with GNPs reversed the increase in alveolar septum thickness due to LPS-induced fibrosis. In addition, treatment with GNPs decreased production of oxidants (nitrite and DCFH), reduced oxidative damage (carbonyl and sulfhydryl), and downregulated activities of superoxide dismutase and catalase. Treatment with GNPs did not showed toxicity; however, it exhibited anti-inflammatory and antioxidative activity that reversed morphological alterations induced by LPS. K E Y W O R D S gold nanoparticles, inflammation, lipopolysaccharide, lung, oxidative stress

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“…Data found in the literature indicate the direct relationship between the increase in the concentration of ROS and the local inflammatory reactions resulting from OA, both considered agents that cause joint wear. The action of neutralizing free radicals obtained by nanoparticles can be explained by the fact that GNPs have positive charges and, therefore, tend to stabilize through interaction with negatively‐charged molecules, neutralizing their action 43 . In addition, both GNP and AH are considered capable of inhibiting NO production and iNOS gene expression 44,45 …”

Section: Discussionmentioning

confidence: 99%

Intra‐articular treatment with hyaluronic acid associated with gold nanoparticles in a mechanical osteoarthritis model in Wistar rats

Filho

Haupenthal

Zaccaron

et al. 2021

Journal Orthopaedic Research

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This study aimed to evaluate the effects of intra‐articular treatment with hyaluronic acid (HA) associated with GNPs in a mechanical model of osteoarthritis induced by median meniscectomy (MM). Fifty Wistar rats (2 months weighing between 250 and 300 g) were used, divided into five groups of 10 animals each: Sham, osteoarthritis (OA), OA + HA, OA + gold nanoparticles (GNPs), and OA + HA + GNPs. Intra‐articular treatment was started 30 days after the model was induced, with a frequency of 2 weeks for 60 days. Fifteen days after the last application, the animals were euthanized with the removal of the joint tissue for biochemical and histological analysis. The model used was able to mimic osteoarthritis, characterized by the presence of high levels of proinflammatory cytokines, oxidative stress, and degeneration of joint surfaces (Grade III, according to SCORE OARSI). The isolated use of HA or GNPs provided beneficial results to the joint; however, only the group subjected to the association between HA and GNPs showed the attenuation of oxidative stress and reduced proinflammatory markers, with a simultaneous increase in levels of anti‐inflammatory cytokines and growth factors. Upon histological analysis, only the OA + HA + GNPs group achieved the restoration of the thickness of the joint cartilage with reduced damage and return to the intact joint surface. The results found demonstrated that the association of GNPs with HA was able to reverse the deleterious effects caused by the model by inhibiting the progressive degeneration of joint surfaces, representing a promising treatment for osteoarthritis.

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Interaction of gold nanoparticles with free radicals and their role in enhancing the scavenging activity of ascorbic acid

Cited by 31 publications

References 34 publications

Hybrid nanocomposite curcumin-capped gold nanoparticle-reduced graphene oxide: Anti-oxidant potency and selective cancer cytotoxicity

Hybrid nanocomposite curcumin-capped gold nanoparticle-reduced graphene oxide: Anti-oxidant potency and selective cancer cytotoxicity

Effects of treatment with gold nanoparticles in a model of acute pulmonary inflammation induced by lipopolysaccharide

Intra‐articular treatment with hyaluronic acid associated with gold nanoparticles in a mechanical osteoarthritis model in Wistar rats

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