Interaction of glia with a compliant, microstructured silicone surface

Minev, Ivan R.; Moshayedi, Pouria; Fawcett, James W.; Lacour, Stéphanie

doi:10.1016/j.actbio.2013.02.048

Cited by 17 publications

(24 citation statements)

References 44 publications

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“…Microglial cell culture purity was quantified after immunolabelling using CD11b antibody (1 : 200; Abcam, Belgium) and found to be above 90% (see details in the electronic supplementary material) in accordance with previous reports that used a similar isolation technique [18,28]. A total of 6 Â 10 4 viable cells ml 21 were seeded on P(TMC-CL) substrates secured at the bottom of a 24-well plate with a silicone o-ring (Epidor, Spain) and cultured in DMEM/F12 medium (Gibco) supplemented with 10% FBS and 1% P/S for 1 or 5 days. Glass coverslips were used as the experimental control.…”

mentioning

confidence: 80%

“…More recently, it was demonstrated that microglia interact mechanically with silicone micropillars on a surface, and are affected by surface stiffness [21].…”

Section: Introductionmentioning

confidence: 99%

“…Cellular ATP content was measured using Celltiter-Glo (Promega, WI, USA), following the manufacturer's instructions. To assess resazurin metabolization, cells were incubated (4 h, 378C) with a resazurin (Sigma-Aldrich) solution (0.1 mg ml 21 , in PBS) and the fluorescence (l ex ¼ 530 nm, l em ¼ 590 nm) in the cell culture medium was measured (Synergy Mx; Biotek, Portugal).…”

Section: Effect Of Microglia-conditioned Media On Astrocyte Metabolicmentioning

confidence: 99%

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The role of the surface on microglia function: implications for central nervous system tissue engineering

Pires

Rocha

Ambrosio

et al. 2015

J. R. Soc. Interface.

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In tissue engineering, it is well accepted that a scaffold surface has a decisive impact on cell behaviour. Here we focused on microglia-the resident immune cells of the central nervous system (CNS)-and on their response to poly(trimethylene carbonate-co-1-caprolactone) (P(TMC-CL)) fibrous and flat surfaces obtained by electrospinning and solvent cast, respectively. This study aims to provide cues for the design of instructive surfaces that can contribute to the challenging process of CNS regeneration. Cell morphology was evidently affected by the substrate, mirroring the surface main features. Cells cultured on flat substrates presented a round shape, while cells with elongated processes were observed on the electrospun fibres. A higher concentration of the pro-inflammatory cytokine tumour necrosis factor-a was detected in culture media from microglia on fibres. Still, astrogliosis is not exacerbated when astrocytes are cultured in the presence of microgliaconditioned media obtained from cultures in contact with either substrate. Furthermore, a significant percentage of microglia was found to participate in the process of myelin phagocytosis, with the formation of multinucleated giant cells being observed only on films. Altogether, the results presented suggest that microglia in contact with the tested substrates may contribute to the regeneration process, putting forward P(TMC-CL) substrates as supporting matrices for nerve regeneration.

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mentioning

confidence: 80%

“…More recently, it was demonstrated that microglia interact mechanically with silicone micropillars on a surface, and are affected by surface stiffness [21].…”

Section: Introductionmentioning

confidence: 99%

Section: Effect Of Microglia-conditioned Media On Astrocyte Metabolicmentioning

confidence: 99%

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The role of the surface on microglia function: implications for central nervous system tissue engineering

Pires

Rocha

Ambrosio

et al. 2015

J. R. Soc. Interface.

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“…Correspondingly, nanostructures also strongly influence the behavior of actin (a self‐assembling protein that forms cell cytoskeletal fibers). Nanopillars and nanoneedles can cause actin stress fiber formation, induce alignment, and the formation of actin caps and rings . This influence has been linked to multiple mechanisms, including the efficacy of hollow nanostraws to penetrate cells for physical intracellular delivery, and the development of membrane‐curvature‐influencing endocytosis (as discussed above) …”

Section: Biomechanical Cuesmentioning

confidence: 99%

High‐Aspect‐Ratio Nanostructured Surfaces as Biological Metamaterials

et al. 2020

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Materials patterned with high‐aspect‐ratio nanostructures have features on similar length scales to cellular components. These surfaces are an extreme topography on the cellular level and have become useful tools for perturbing and sensing the cellular environment. Motivation comes from the ability of high‐aspect‐ratio nanostructures to deliver cargoes into cells and tissues, access the intracellular environment, and control cell behavior. These structures directly perturb cells' ability to sense and respond to external forces, influencing cell fate, and enabling new mechanistic studies. Through careful design of their nanoscale structure, these systems act as biological metamaterials, eliciting unusual biological responses. While predominantly used to interface eukaryotic cells, there is growing interest in nonanimal and prokaryotic cell interfacing. Both experimental and theoretical studies have attempted to develop a mechanistic understanding for the observed behaviors, predominantly focusing on the cell–nanostructure interface. This review considers how high‐aspect‐ratio nanostructured surfaces are used to both stimulate and sense biological systems.

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“…We tested the effect of the hybrid cell‐gel system in vitro then in vivo against cell viability and peripheral nerve sprouting. We selected polydimethylsiloxane (PDMS) as an inert in vitro substrate and in vivo construct material (Minev, Moshayedi, Fawcett, & Lacour, , Minev et al, ; Srinivasan et al, ). Although alternative conduit materials such as biodegradable fibrin are often used to prepare regenerative nerve conduits, we opted for PDMS, a material of choice for cuff electrodes, in the prospect of subsequent regenerative nerve electrode implants.…”

Section: Introductionmentioning

confidence: 99%

In vitro evaluation of gel‐encapsulated adipose derived stem cells: Biochemical cues for in vivo peripheral nerve repair

Luca

Fonta

Raffoul

et al. 2017

J Tissue Eng Regen Med

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Adipose-derived stem cells (ASC) are becoming one of the most exploited cells in peripheral nerve repair. They are fast-growing and able to protect neurons from apoptosis; they can reduce postinjury latency and the risk of muscle atrophy. This study evaluates laminin-loaded fibrin gel as an ASC-carrying scaffold for nerve repair. In vitro, ASC retained their proliferative activity but showed significant increase in proliferation rate when encapsulated in gels with low laminin concentrations (i.e., 1 μg/mL). We observed a linear decrease of ASC proliferation rate with increasing laminin concentration from 1 to 100 μg/mL. We next examined the effect of the ASC-carrying fibrin gels on in vitro dorsal root ganglia (DRG) neurite extension, then in vivo sciatic nerve regeneration in adult rats. The ASC-carrying gel was embedded in 15-mm-long, 1.5-mm-diameter polydimethylsiloxane regenerative conduits for in vivo evaluation. At 8-week post implantation, robust regeneration was observed across the long gap. Taken together, these results suggest ASC-carrying gels are a potential path to improve the efficacy of nerve regeneration through artificial guidance conduits and electrode nerve interfaces.

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Interaction of glia with a compliant, microstructured silicone surface

Cited by 17 publications

References 44 publications

The role of the surface on microglia function: implications for central nervous system tissue engineering

The role of the surface on microglia function: implications for central nervous system tissue engineering

High‐Aspect‐Ratio Nanostructured Surfaces as Biological Metamaterials

In vitro evaluation of gel‐encapsulated adipose derived stem cells: Biochemical cues for in vivo peripheral nerve repair

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