In vitro evaluation of gel‐encapsulated adipose derived stem cells: Biochemical cues for in vivo peripheral nerve repair

Luca, Alba C. de; Fonta, Caroline; Raffoul, Wassim; Summa, Pietro G. di; Lacour, Stéphanie

doi:10.1002/term.2486

Cited by 14 publications

(16 citation statements)

References 50 publications

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“…ASCs were harvested from Sprague–Dawley rats (Janvier, France) as described earlier(de Luca et al, ) and maintained in Modified Eagle Medium (α‐MEM; Invitrogen, UK) containing 1% (v/v) streptomycin/penicillin solution and 10% (v/v) FBS. ASCs were differentiated at early passages, i.e., 4–6.…”

Section: Methodsmentioning

confidence: 99%

“…Suboptimal outcome is attributed to axonal degeneration, fibrotic scar formation, and neuromas at the site of injury (Ngeow, ). Previous research has focused on the different cellular and structural components of the regenerating nich e and managed to improve peripheral nerve regeneration through axonal guidance with neural conduits (Zhang et al, ; Wang et al, ), growth factor delivery (Madduri et al, ), cell therapy hydrogels (Masand et al, ) and extracellular matrix molecules (de Luca et al, ; de Luca et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…Previous work of the group focused on fibrin conduits enriched with SC‐like differentiated adipose derived stem cells (dASCs) in order to enhance the microenvironment and promote effective axonal regeneration (di Summa et al, ). Furthermore, Polydimethylsiloxane (PDMS) conduits enriched with adipose derived stem cells enhanced axonal regeneration over a critical distance of 15 mm (de Luca et al, ). Despite the vast literature reported on possible elements for improving nerve regeneration, studies on collagen infiltration and scar tissue formation in regenerating nerves are scarce.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Adipose Derived Stem Cells Reduce Fibrosis and Promote Nerve Regeneration in Rats

Summa

Schiraldi

Cherubino

et al. 2018

The Anatomical Record

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Peripheral nerve regeneration is critical and challenging in the adult humans. High level of collagen infiltration (i.e., scar tissue), in the niche of injury, impedes axonal regeneration and path finding. Unfortunately, studies focusing on the modulation of scar tissue in the nerves are scarce. To address part of this problem, we have evaluated the differentiated adipose derived stem cells (dASCs) for their antifibrotic and regenerative effects in a 10 mm nerve gap model in rats. Three different animal groups (N = 5) were treated with fibrin nerve conduits (empty), or seeded with dASCs (F + dASCs) and autograft, respectively. Histological analysis of regenerated nerves, at 12 weeks postoperatively, reveled the high levels of collagen infiltration (i.e., 21.5% ± 6.1% and 24.1% ± 2.9%) in the middle and distal segment of empty conduit groups in comparison with stem cells treated (16.6% ± 2.1% and 12.1% ± 2.9%) and autograft (15.0% ± 1.7% and 12.8% ± 1.0%) animals. Thus, the dASCs treatment resulted in significant reduction of fibrotic tissue formation. Consequently, enhanced axonal regeneration and remyelination was found in the animals treated with dASCs. Interestingly, these effects of dASCs appeared to be equivalent to that of autograft treatment. Thus, the dASCs hold great potential for preventing the scar tissue formation and for promoting nerve regeneration in the adult organisms. Future experiments will focus on the validation of these findings in a critical nerve injury model. Anat Rec, 301:1714-1721, 2018. © 2018 Wiley Periodicals, Inc.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Adipose Derived Stem Cells Reduce Fibrosis and Promote Nerve Regeneration in Rats

Summa

Schiraldi

Cherubino

et al. 2018

The Anatomical Record

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“…Мезенхімальні стовбурові клітини кісткового мозку (МСК-КМ) позбавлені таких недоліків, як туморогенність і морально-етичні проблеми, можуть бути отримані за допомогою пункції кісткового мозку, а в спеціальних умовах культивування можуть диференціюватися у нейрони, астроцити та шванівські клітини [96,97]. Багато дослідників показали, що використання МСК-КМ сумісно з матриксами для тубажу нерва чи ацелюлярними нервами дає кращі результати ніж використання самих порожнистих матриксів без МСК-КМ [74, 98-100], інші, що позитивний ефект від застосування МСК-КМ гірший ніж при використанні культивованих шванівських клітин [101], але більшість погоджуються з думкою, що ефект приблизно однаковий [102,103].…”

Section: джерела і види стовбурових клітин для відновлення периферичнunclassified

Stem Cell Technology in Peripheral Nerve Restoration

Petriv

Tsymbalyuk

Potapov

et al. 2020

EUMJ

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Peripheral nerve injuries are a significant problem in the medical and socio-economic plan, as they are accompanied by a high incidence of disability by people of working age. In recent decades, significant progress has been made in the restorative surgery of the peripheral nervous system, in particular through the introduction into clinical practice of microsurgical techniques. However, the problem of restoring the peripheral nerve after its traumatic injury has not been resolved yet. A review article addresses the current state of developing stem cell technologies for peripheral nerve repair. Basic concepts of peripheral nerve regeneration after traumatic injury, methods of their restoration in experimental and clinic conditions are considered. The prospect of using stem cells of different origins is shown in the experiment by many authors, and the positive effect of stem cells on peripheral nerve regeneration is explained by their ability to secrete many trophic factors and differentiation to a neural phenotype. An essential issue in the tissue engineering approach is the choice of the optimal material to be used as a scaffold for large size peripheral nerve defects grafting. The article focuses on the main types of stem cells, as well as their combinations with biopolymers, which have shown efficiency in the experiment. Despite the advances in the use of the latest technologies, the search for the necessary components is underway to provide the most favorable conditions for peripheral nerve regeneration in the clinic.

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“…For instance, silicone (polydimethylsiloxane) tubes, encapsulating adipose-derived stem cells and inserted between nerve stumps, reportedly induced regeneration in rat sciatic nerves. 18 A bioabsorbable polyglycolic acid tube filled with collagen sponge enabled the reconnection of canine peroneal nerves. 19 Such tubes have also been investigated in clinical studies and were reported to improve functional nerve regeneration.…”

Section: Introductionmentioning

confidence: 99%

Recurrent laryngeal nerve regeneration using a self‐assembling peptide hydrogel

et al. 2019

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Objectives/Hypothesis To regenerate defected recurrent laryngeal nerves (RLNs), various methods have been developed. However, no consistently effective treatments are currently available because of their insufficient functional recovery. RADA16‐I, a self‐assembling peptide used clinically as a hemostat, reportedly supports neurite outgrowth and functional synapse formation in vitro. The purpose of this study was to investigate the effect of RADA16‐I hydrogels on transected RLNs in rats. Study Design Animal experiments with controls. Methods Fifteen adult rats were divided into the following three groups: RADA16‐I (+), RADA16‐I (−), and neurectomy. A 6‐mm gap of the left RLN was bridged using an 8‐mm silicone tube in the RADA16‐I (−) and RADA16‐I (+) groups. Subsequently, RADA16‐I hydrogel was injected into the tube in the RADA16‐I (+) group. The surgical incisions were closed without any further treatment in the neurectomy group. After 8 weeks, laryngoscopy and electrophysiological and histological examinations were performed to evaluate the effect of RADA16‐I on nerve regeneration and thyroarytenoid muscle atrophy. Results Although most rats in the three groups exhibited no improvements of their vocal fold movement, partial recovery was observed in one rat in the RADA16‐I (+) group. The neurofilament‐positive areas and the number of myelinated nerves in the RADA16‐I (+) group were significantly higher than in the RADA16‐I (−) group. The area of the left thyroarytenoid muscle in the RADA16‐I (+) group was significantly larger than that of the neurectomy group. Conclusions Our results suggested that RADA16‐I hydrogel was effective for RLN regeneration. Level of Evidence NA Laryngoscope, 130:2420–2427, 2020

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In vitro evaluation of gel‐encapsulated adipose derived stem cells: Biochemical cues for in vivo peripheral nerve repair

Cited by 14 publications

References 50 publications

Adipose Derived Stem Cells Reduce Fibrosis and Promote Nerve Regeneration in Rats

Adipose Derived Stem Cells Reduce Fibrosis and Promote Nerve Regeneration in Rats

Stem Cell Technology in Peripheral Nerve Restoration

Recurrent laryngeal nerve regeneration using a self‐assembling peptide hydrogel

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