Interaction of Fungal Polygalacturonase with Plant Proteins in Relation to Specificity and Regulation of Plant Defense Response

Cervone, Felice; Lorenzo, Giulia De; Degrà, L.; Salvi, G.

doi:10.1007/978-3-642-71652-2_24

Cited by 16 publications

(6 citation statements)

References 6 publications

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“…A dual role for XIs in plant protection was hypothesized: that they may counteract a pathogenic attack, directly by reducing the hydrolytic activity of the excreted xylanases and, beyond that, they may lead to a more balanced production of elicitor-active arabino(xylo)-oligosaccharides. These smaller fragments may, in turn, trigger the activation of downstream defensive processes within the plant, as previously described for polygalacturonase inhibitors (PGIPs; Cervone et al, 1986;Di et al, 2006). Apart from being constitutively expressed, it has been demonstrated that some XI-encoding genes are induced (Igawa et al, 2004).…”

Section: Discussionmentioning

confidence: 92%

The three classes of wheat xylanase-inhibiting proteins accumulate in an analogous way during wheat ear development and germination

Croes

Gebruers

Luyten

et al. 2009

Journal of Plant Physiology

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Section: Discussionmentioning

confidence: 92%

The three classes of wheat xylanase-inhibiting proteins accumulate in an analogous way during wheat ear development and germination

Croes

Gebruers

Luyten

et al. 2009

Journal of Plant Physiology

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“…In vitro, PGIP modulates endopolygalacturonase activity and favours the accumulation of elicitor-active oligogalacturonides (Cervone et al 1986). On the basis of this ability to convert a fungal pathogenicity factor into an elicitor of plant defense responses, PGIP has been implicated in resistance to pathogenic fungi.…”

Section: Introductionmentioning

confidence: 98%

Developmental and pathogen-induced accumulation of transcripts of polygalacturonase-inhibiting protein in Phaseolus vulgaris L.

Devoto

Clark

Nuss

et al. 1997

Planta

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Expression of the polygalacturonase-inhibiting-protein gene ( pgip) during development of French bean (Phaseolus vulgaris L. cv. Red Kidney) was analysed in various organs, including leaves, stems and roots of etiolated and light-grown seedlings, light-grown adult plants, as well as¯owers, pods, and seeds. In lightgrown seedlings and plants, pgip transcripts were detected in all tissues examined, with higher levels found in the transition zone between the elongating and mature region of young hypocotyls, and in the basal region of the stem of adult plants. In etiolated hypocotyls, levels of pgip transcripts in the elongating region were ®ve-to six fold higher than in light-grown hypocotyls. High levels of transcripts were also observed in pods. Accumulation of pgip mRNA was also followed in both compatible and incompatible race-cultivar interactions between Colletotrichum lindemuthianum races a and c, respectively, and P. vulgaris by Northern blot analysis and in situ hybridisation. In situ experiments showed, in incompatible interactions, a rapid, intense and transient accumulation of pgip mRNA in epidermal cells proximal to the site of infection, and, less intense, within the cortical parenchyma underneath. In compatible interactions, no substantial accumulation of pgip mRNA was observed in hypocotyls; a very weak accumulation was observed in leaves during lesion formation.

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“…between 10 and 15 are potent elicitors of plant defense responses, but are only transiently produced by the action of EPG in vitro. A polygalacturonase-inhibiting protein (PGIP) has been found to be present in the cell walls of all dicotyledonous plants that have been examined (Albersheim and Anderson, 1971;Cervone et a/., 1986;. In vjtro experiments have demonstrated that addition of PGlP to fungal EPG in the presence of polygalacturonic acid results in the prolonged existence in the reaction mixture of elicitor-active oligogalacturonides (d.p.s.…”

Section: Introductionmentioning

confidence: 99%

Polygalacturonase‐inhibiting protein accumulates in Phaseolus vulgaris L. in response to wounding, elicitors and fungal infection

et al. 1994

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Summary Polygalacturonase‐inhibiting protein (PGIP) is a cell wall‐associated protein that specifically binds to and inhibits the activity of fungal endopolygalacturonases. The Phaseolus vulgaris gene encoding PGIP has been cloned and characterized. Using a fragment of the cloned pgip gene as a probe in Northern blot experiments, it is demonstrated that the pgip mRNA accumulates in suspension‐cultured bean cells following addition of elicitor‐active oligogalacturonides or fungal glucan to the medium. Rabbit polyclonal antibodies specific for PGIP were generated against a synthetic peptide designed from the N‐terminal region of PGIP; the antigenicity of the peptide was enhanced by coupling to KLH. Using the antibodies and the cloned pgip gene fragment as probes in Western and Northern blot experiments, respectively, it is shown that the levels of PGIP and its mRNA are increased in P. vulgaris hypocotyls in response to wounding or treatment with salicylic acid. Using gold‐labeled goat‐anti‐rabbit secondary antibodies in EM studies, it has also been demonstrated that, in bean hypocotyls infected with Colletotrichum lindemuthianum, the level of PGIP preferentially increases in those cells immediately surrounding the infection site. The data support the hypothesis that synthesis of PGIP constitutes an active defense mechanism of plants that is elicited by signal molecules known to induce plant defense genes.

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Interaction of Fungal Polygalacturonase with Plant Proteins in Relation to Specificity and Regulation of Plant Defense Response

Cited by 16 publications

References 6 publications

The three classes of wheat xylanase-inhibiting proteins accumulate in an analogous way during wheat ear development and germination

The three classes of wheat xylanase-inhibiting proteins accumulate in an analogous way during wheat ear development and germination

Developmental and pathogen-induced accumulation of transcripts of polygalacturonase-inhibiting protein in Phaseolus vulgaris L.

Polygalacturonase‐inhibiting protein accumulates in Phaseolus vulgaris L. in response to wounding, elicitors and fungal infection

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