Interaction of diethyl aniline methylphosphonate with DNA: Spectroscopic and isothermal titration calorimetry

Lü, Yan; Xu, Mengnan; Wang, Gongke; Zheng, Yun

doi:10.1016/j.jlumin.2010.12.025

Cited by 22 publications

(4 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are a number of techniques used to study the interaction of small molecules with DNA, such as fluorescence spectroscopy, UV spectrophotometry, electrochemical methods [11], circular dichroism spectroscopy [12] and isothermal titration calorimetry [13]. Among these methods, UV absorption and fluorescence spectroscopy are well known as simple but sensitive methods.…”

Section: Introductionmentioning

confidence: 99%

Interaction of 3′-azido-3′-deamino daunorubicin with DNA: Multispectroscopic and molecular modeling

Cui

Hui

Jiang

et al. 2012

International Journal of Biological Macromolecules

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Interaction of 3′-azido-3′-deamino daunorubicin with DNA: Multispectroscopic and molecular modeling

Cui

Hui

Jiang

et al. 2012

International Journal of Biological Macromolecules

View full text Add to dashboard Cite

“…The crystal structures of ctDNA (PDB entry code is 453D) and HSA (PDB entry code is 1H9Z) used for molecular modeling were extracted from the Protein Data Bank . Water was removed from the DNA and HSA PDB file.…”

Section: Methodsmentioning

confidence: 99%

Molecular interaction of ctDNA and HSA with sulfadiazine sodium by multispectroscopic methods and molecular modeling

Geng

Liu

et al. 2013

Luminescence

View full text Add to dashboard Cite

Interactions of sulfadiazine sodium (SD-Na) with calf thymus DNA (ctDNA) and human serum albumin (HSA) were studied using fluorescence spectroscopy, UV absorption spectroscopy and molecular modeling. The fluorescence experiments showed that the processes were static quenching. The results of UV spectra and molecular modeling of the interaction between SD-Na and ctDNA indicated that the binding mode might be groove binding. In addition, the interaction of SD-Na with HSA under simulative physiological conditions was also investigated. The binding constants (K) and the number of binding sites (n) at different temperatures (292, 302, 312 K) were 5.23 × 10(3) L/mol, 2.18; 4.50 × 10(3) L/mol, 2.35; and 4.08 × 10(3) L/mol, 2.47, respectively. Thermodynamic parameters including enthalpy change (ΔH) and entropy change (ΔS) were calculated, the results suggesting that hydrophobic force played a very important role in SD-Na binding to HSA, which was in good agreement with the molecular modeling study. Moreover, the effect of SD-Na on the conformation of HSA was analyzed using three-dimensional fluorescence spectra.

show abstract

“…Upon each injection of 2 μL DMP, the ITC curve shows a thermal signal corresponding to the heat released from the DMP-eARGs binding (Figure 2A), and the peak intensity decreased over time with a distinct binding jump. 23,28 Analysis of the integral heat provided further thermodynamic insights into the DMP-eARGs binding mode. The enthalpy change (ΔH) of −0.032 kcal/mol and entropy change (ΔS) of 0.024 kcal/mol/deg (Figure 2A) suggests that their binding is a slightly exothermic and entropydriven process.…”

Section: Changes Of Bacterial Transformation Frequency Inmentioning

confidence: 99%

Phthalates Boost Natural Transformation of Extracellular Antibiotic Resistance Genes through Enhancing Bacterial Motility and DNA Environmental Persistence

Wu,

Lv,

Sun

et al. 2024

Environ. Sci. Technol.

View full text Add to dashboard Cite

The environmental dissemination of extracellular antibiotic resistance genes (eARGs) in wastewater and natural water bodies has aroused growing ecological concerns. The coexisting chemical pollutants in water are known to markedly affect the eARGs transfer behaviors of the environmental microbial community, but the detailed interactions and specific impacts remain elusive so far. Here, we revealed a concentration-dependent impact of dimethyl phthalate (DMP) and several other types of phthalate esters (common water pollutants released from plastics) on the natural transformation of eARGs. The DMP exposure at an environmentally relevant concentration (10 μg/L) resulted in a 4.8-times raised transformation frequency of Acinetobacter baylyi but severely suppressed the transformation at a high concentration (1000 μg/L). The promotion by low-concentration DMP was attributed to multiple mechanisms, including increased bacterial mobility and membrane permeability to facilitate eARGs uptake and improved resistance of the DMP-bounded eARGs (via noncovalent interaction) to enzymatic degradation (with suppressed DNase activity). Similar promoting effects of DMP on the eARGs transformation were also found in real wastewater and biofilm systems. In contrast, higher-concentration DMP suppressed the eARGs transformation by disrupting the DNA structure. Our findings highlight a potentially underestimated eARGs spreading in aquatic environments due to the impacts of coexisting chemical pollutants and deepen our understanding of the risks of biological-chemical combined pollution in wastewater and environmental water bodies.

show abstract

Interaction of diethyl aniline methylphosphonate with DNA: Spectroscopic and isothermal titration calorimetry

Cited by 22 publications

References 43 publications

Interaction of 3′-azido-3′-deamino daunorubicin with DNA: Multispectroscopic and molecular modeling

Interaction of 3′-azido-3′-deamino daunorubicin with DNA: Multispectroscopic and molecular modeling

Molecular interaction of ctDNA and HSA with sulfadiazine sodium by multispectroscopic methods and molecular modeling

Phthalates Boost Natural Transformation of Extracellular Antibiotic Resistance Genes through Enhancing Bacterial Motility and DNA Environmental Persistence

Contact Info

Product

Resources

About